2020
DOI: 10.1186/s12934-020-01485-z
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Engineering of a genome-reduced strain Bacillus amyloliquefaciens for enhancing surfactin production

Abstract: Background Genome reduction and metabolic engineering have emerged as intensive research hotspots for constructing the promising functional chassis and various microbial cell factories. Surfactin, a lipopeptide-type biosurfactant with broad spectrum antibiotic activity, has wide application prospects in anticancer therapy, biocontrol and bioremediation. Bacillus amyloliquefaciens LL3, previously isolated by our lab, contains an intact srfA operon in the genome for surfactin biosynthesis. … Show more

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Cited by 46 publications
(25 citation statements)
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“…In E. coli , production of L-threonine in a genome-reduced strain was 83% higher than that in wild-type strain 5 . Likewise, a genome-reduced strain of Bacillus amyloliquefaciens lacking ~4.18% of the genome showed 10.4-fold increase in surfactin biosynthesis compared to the parent strain 30 . Interestingly, deletion of 7.7% of the genome of Pseudomonas mendocina NK-01 improved the ATP/ADP ratio of the cell by 11 times and enhanced MCL-PHA accumulation by 114.8% 31 .…”
Section: Discussionmentioning
confidence: 99%
“…In E. coli , production of L-threonine in a genome-reduced strain was 83% higher than that in wild-type strain 5 . Likewise, a genome-reduced strain of Bacillus amyloliquefaciens lacking ~4.18% of the genome showed 10.4-fold increase in surfactin biosynthesis compared to the parent strain 30 . Interestingly, deletion of 7.7% of the genome of Pseudomonas mendocina NK-01 improved the ATP/ADP ratio of the cell by 11 times and enhanced MCL-PHA accumulation by 114.8% 31 .…”
Section: Discussionmentioning
confidence: 99%
“…This strain showed significant improvements in heterologous gene expression with increased yields of cellulase (1.7-fold) and protease (2.5-fold) ( Morimoto et al, 2008 ). In Bacillus amyloquefaciens , 4.18% of the genome was deleted, making strain GR167, improving transformation efficiency, growth rates, and heterologous gene expression ( Zhang et al, 2020 ). These growth characteristics highlight this strain as a suitable chassis for further genetic modification and industrial applications.…”
Section: Applications Of Reduced-genome Bacterial Strainsmentioning
confidence: 99%
“…As a proof of concept, GR167 was engineered to produce surfactin with two deletions and introduction of a stronger promoter in front of the native surfactant producing gene. These modifications, making strain GR167IDS, resulted in a 10.4-fold increase in surfactin compared to GR167 ( Zhang et al, 2020 ). This highlights both the ease of manipulation of the reduced genome strain as well as the benefit of combining these reductions with other modifications to improve the expression of either native or foreign genes.…”
Section: Applications Of Reduced-genome Bacterial Strainsmentioning
confidence: 99%
“…To increase the production of heterologous proteins, numerous studies have focused on the design and construction of an optimized functional microbial cell chassis with a reduced genome and streamlined physiological characteristics ( Zhang F. et al, 2020 ). To date, several model strains have been intensively researched due to their clear genetic background, and efficient genome editing approaches have been applied in species such as B. subtilis and B. licheniformis for the production of heterologous proteins by rational microbial chassis engineering ( Contesini et al, 2017 ; Mizoguchi et al, 2018 ; Aguilar Suarez et al, 2019 ).…”
Section: Introductionmentioning
confidence: 99%