Engineering of New UV-Blocking Hollow Proteinoid Nanoparticles of Narrow Size Distribution Containing All-trans Retinoic Acid for Biomedical Applications

Belostozky, Anna; Kolitz‐Domb, Michal; Grinberg, Igor; Haham, Hai; Margel, Shlomo

doi:10.4172/2157-7439.1000462

Cited by 4 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The synthesized proteinoids were of relatively high molecular weight with relatively narrow polydispersity index (PDI)36 kDa and 1.02 PDI, 68 kDa, and 1.04 PDI, and 95 kDa and 1.09 PDI for poly(KI), poly(EI), and poly(KF), respectively. This indicates that the simple thermal step-growth polymerization procedure used here provides relatively long polymer chains with narrow PDI, as reported in previous studies by our group. ,− , Furthermore, Table shows that all proteinoids exhibit optical activity, although the monomers are known to racemize during the thermal process …”

Section: Resultssupporting

confidence: 85%

“…This indicates that the simple thermal step-growth polymerization procedure used here provides relatively long polymer chains with narrow PDI, as reported in previous studies by our group. 5,11−14,19 Furthermore, Table 1 shows that all proteinoids exhibit optical activity, although the monomers are known to racemize during the thermal process. 20…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Engineering of Durable Antifog Thin Coatings on Plastic Films by UV-Curing of Proteinoid Prepolymers with PEG-Diacrylate Monomers

et al. 2019

Self Cite

View full text Add to dashboard Cite

Fog formation on transparent surfaces constitutes a major challenge in several optical applications, such as plastic packaging, lenses, mirrors, and windshields. To overcome this problem, we prepared and characterized durable antifog thin coatings on plastic films such as polyethylene terephthalate (PET). Proteinoids are biocompatible random polymers made of α-amino acids by thermal step-growth polymerization. Proteinoid prepolymers were prepared by adding activated double bonds to proteinoids via the Michael addition reaction. A series of thin antifog cross-linked coatings were prepared by spreading on PET films with a Mayer rod various mixtures of the proteinoid prepolymers, polyethylene glycol diacrylate, and a photoinitiator, followed by UV-curing of the dried coatings. The antifog properties of the coatings were determined by the contact angle, roughness, haze, and gloss measurements, as well as hot and cold fog tests, to examine the optical properties of the films under fog formation conditions. Mechanical properties such as adhesion, robustness, and abrasion resistance of the antifog coatings were examined by tape, knife-scratch, and sandpaper abrasion tests. The effect of coating composition, wettability, and roughness on the antifog properties of the coated PET films was elucidated. The formula was optimized, and the corresponding UV-cured antifog cross-linked thin coating exhibited transparency with good adhesion and excellent durable antifog performance.

show abstract

Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Engineering of Durable Antifog Thin Coatings on Plastic Films by UV-Curing of Proteinoid Prepolymers with PEG-Diacrylate Monomers

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The spectrum of Pal (Figure S2B) demonstrates typical peaks at 1900-1640 cm −1 corresponding to the carbonyl stretching band, 2780-2960 cm −1 (C-H and CH 3 stretching band [55]) and 3500 cm −1 (N-H stretching band [54]). The spectrum of P(RGD) (Figure S2C) demonstrates typical peaks of proteinoids [21,50] at 660 and 770 cm −1 (amine N-H wagging [56]) belonging to the arginine side chain, which possesses three atoms of nitrogen. The peaks at 1050 and 1200 cm −1 correspond to the C-N stretching band, the peak at 1560 cm −1 corresponds to amide N-H stretching band, and the broad peak at 2700-3500 cm −1 can be attributed to C-H, N-H and O-H stretching bands [32].…”

Section: Ftir Analysis Of the Free Drugs And P(rgd) Proteinoid Ncsmentioning

confidence: 99%

“…Suitable molecules can be encapsulated within the internal core of the formed particle owing to hydrophobic residues; a marker (e.g., a fluorescent label) can be conjugated to the surface using hydrophilic residues such as amines [20]. Previous studies presented the encapsulation of various molecules for cosmetics (retinoic acid [21]), chemotherapeutics (doxorubicin and Paclitaxel [18,22,23], cannabidiol [24]), antipsychotics (Risperidone [25]) and dyes for diagnostic imaging (ICG [26,27]). D-arginine (R), glycine (G) and L-aspartic acid (D) were used for randomly achieving the RGD sequence within the proteinoid backbone; previous studies showed that 13% of the established proteinoids contain the RGD sequence [26,28].…”

Section: Introductionmentioning

confidence: 99%

Tumor-Targeted Fluorescent Proteinoid Nanocapsules Encapsulating Synergistic Drugs for Personalized Cancer Therapy

et al. 2021

Self Cite

View full text Add to dashboard Cite

Personalized cancer treatment based on specific mutations offers targeted therapy and is preferred over “standard” chemotherapy. Proteinoid polymers produced by thermal step-growth polymerization of amino acids may form nanocapsules (NCs) that encapsulate drugs overcoming miscibility problems and allowing passive targeted delivery with reduced side effects. The arginine-glycine-glutamic acid (RGD) sequence is known for its preferential attraction to αvβ3 integrin, which is highly expressed on neovascular endothelial cells that support tumor growth. Here, tumor-targeted RGD-based proteinoid NCs entrapping a synergistic combination of Palbociclib (Pal) and Alpelisib (Alp) were synthesized by self-assembly to induce the reduction of tumor cell growth in different types of cancers. The diameters of the hollow and drug encapsulating poly(RGD) NCs were 34 ± 5 and 22 ± 3 nm, respectively; thereby, their drug targeted efficiency is due to both passive and active targeting. The encapsulation yield of Pal and Alp was 70 and 90%, respectively. In vitro experiments with A549, MCF7 and HCT116 human cancer cells demonstrate a synergistic effect of Pal and Alp, controlled release and dose dependence. Preliminary results in a 3D tumor spheroid model with cells derived from patient-derived xenografts of colon cancer illustrate disassembly of spheroids, indicating that the NCs have therapeutic potential.

show abstract

Proteinoid Nanocapsules as Drug Delivery System for Improving Antipsychotic Activity of Risperidone

et al. 2020

Self Cite

View full text Add to dashboard Cite

Risperidone (RSP) is an atypical antipsychotic drug widely used to treat schizophrenia and bipolar disorder. Nanoparticles (NPs) are being developed as in vivo targeted drug delivery systems, which cross the blood-brain barrier and improve pharmacokinetics and drug effectiveness. Here, biodegradable proteinoids were synthesized by thermal step-growth polymerization from the amino acids l-glutamic acid, l-phenylalanine and l-histidine and poly (l-lactic acid). Proteinoid NPs containing RSP were then formed by self-assembly, overcoming the insolubility of the drug in water, followed by PEGylation (poly ethylene glycol (PEG) conjugation to increase the stability of the NPs in the aqueous continuous phase. These NPs are biodegradable owing to their peptide and ester moieties. They were characterized in terms of diameter, size distribution, drug loading, and long-term storage. Behavioral studies on mice found enhanced antipsychotic activity compared to free RSP.

show abstract

Engineering of New UV-Blocking Hollow Proteinoid Nanoparticles of Narrow Size Distribution Containing All-trans Retinoic Acid for Biomedical Applications

Cited by 4 publications

References 29 publications

Engineering of Durable Antifog Thin Coatings on Plastic Films by UV-Curing of Proteinoid Prepolymers with PEG-Diacrylate Monomers

Engineering of Durable Antifog Thin Coatings on Plastic Films by UV-Curing of Proteinoid Prepolymers with PEG-Diacrylate Monomers

Tumor-Targeted Fluorescent Proteinoid Nanocapsules Encapsulating Synergistic Drugs for Personalized Cancer Therapy

Proteinoid Nanocapsules as Drug Delivery System for Improving Antipsychotic Activity of Risperidone

Contact Info

Product

Resources

About