Engineering organophosphate hydrolase for enhanced biocatalytic performance: A review

Wang, Lijie; Sun, Yan

doi:10.1016/j.bej.2021.107945

Cited by 25 publications

(15 citation statements)

References 125 publications

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“…Among these, the main components of some chemical weapons are composed of organophosphorus nerve agents, which pose a potential threat to human health. , Hence, great efforts have been made to efficiently degrade organophosphorus nerve agents. The biodegradation method represented by organophosphorus hydrolase has attracted extensive attention because of its high efficiency and environmental friendliness . Preliminary experiments on the degradation of methyl parathion using the Pd@Tz-Da@OPH were carried out, and the results are shown in Figure S12.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The biodegradation method represented by organophosphorus hydrolase has attracted extensive attention because of its high efficiency and environmental friendliness. 48 Preliminary experiments on the degradation of methyl parathion using the Pd@Tz-Da@OPH were carried out, and the results are shown in Figure S12. In the first step of cascade reaction process, OPH in Pd@Tz-Da@ OPH degraded methyl parathion into 4-NP (yellow substance) and dimethyl phosphorothioate (Figure S12a).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Metal Nanoparticles@Covalent Organic Framework@Enzymes: A Universal Platform for Fabricating a Metal–Enzyme Integrated Nanocatalyst

Zhao

Liu

et al. 2022

ACS Appl. Mater. Interfaces

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Cascade catalysis that combines chemical catalysis and biocatalysis has received extensive attention in recent years, especially the integration of metal nanoparticles (MNPs) with enzymes. However, the compatibility between MNPs and enzymes, and the stability of the integrated nanocatalyst should be improved to promote the application. Therefore, in this study, we proposed a strategy to space-separately co-immobilize MNPs and enzymes to the pores and surface of a highly stable covalent organic framework (COF), respectively. Typically, Pd NPs that were prepared by in situ reduction with triazinyl as the nucleation site were distributed in COF (Tz-Da), and organophosphorus hydrolase (OPH) was immobilized on the surface of Tz-Da by a covalent method to improve its stability. The obtained integrated nanocatalyst Pd@Tz-Da@OPH showed high catalytic efficiency and reusability in the cascade degradation of organophosphate nerve agents. Furthermore, the versatility of the preparation strategy of COF-based integrated nanocatalyst has been preliminarily expanded: (1) Pd NPs and OPH were immobilized in the triazinyl COF (TTB-DHBD) with different pore sizes for cascade degradation of organophosphate nerve agent and the particle size of MNPs can be regulated. (2) Pt NPs and glucose oxidase were immobilized in COF (Tz-Da) to obtain an integrated nanocatalyst for efficient colorimetric detection of phenol.

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Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Metal Nanoparticles@Covalent Organic Framework@Enzymes: A Universal Platform for Fabricating a Metal–Enzyme Integrated Nanocatalyst

Zhao

Liu

et al. 2022

ACS Appl. Mater. Interfaces

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“…Similarly, fluctuation in the bodily temperature may affect the enzyme activity by either affecting their activation energy or thermal stability. Another example is organophosphorus acid anhydrolase (OPAA), which is used to detect toxic organophosphorus compounds [ 70 ]. One of the main issues with OPAA is that their optimal operating temperature is 45 °C, which is significantly higher than the human body temperature, thus limiting their practical use in wearable biosensors.…”

Section: Biorecognition Requirements—nucleic Acid-based Assaysmentioning

confidence: 99%

Recent Advances, Opportunities, and Challenges in Developing Nucleic Acid Integrated Wearable Biosensors for Expanding the Capabilities of Wearable Technologies in Health Monitoring

et al. 2022

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Wearable biosensors are becoming increasingly popular due to the rise in demand for non-invasive, real-time monitoring of health and personalized medicine. Traditionally, wearable biosensors have explored protein-based enzymatic and affinity-based detection strategies. However, in the past decade, with the success of nucleic acid-based point-of-care diagnostics, a paradigm shift has been observed in integrating nucleic acid-based assays into wearable sensors, offering better stability, enhanced analytical performance, and better clinical applicability. This narrative review builds upon the current state and advances in utilizing nucleic acid-based assays, including oligonucleotides, nucleic acid, aptamers, and CRISPR-Cas, in wearable biosensing. The review also discusses the three fundamental blocks, i.e., fabrication requirements, biomolecule integration, and transduction mechanism, for creating nucleic acid integrated wearable biosensors.

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“…By replacing cholinesterase with an organophosphate hydrolase enzyme as recognition elements, a faster detection can be obtained but with a lower sensitivity. Until now, various electrochemical sensors have been developed based on the use of organophosphorus hydrolase as receptors, with the detection limit of approximately 0.1 μM [20][21][22]. However, the commercial application of enzyme-based sensors has been obstructed by the high cost of enzyme extraction, purification, and processing [23].…”

Section: Electrochemical Sensing Of Cwasmentioning

confidence: 99%

Electrochemical Sensors for the Detection of Chemical Warfare Agents: Strategy and Exemples

Stoian

Moşteanu

Ilie

2022

International Conference KNOWLEDGE-BASED ORGANIZATION

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Since the addition of chemical warfare agents (CWAs) in the World War I, there has been a constant demand for the development of rapid and accurate analytical instruments for detecting and identifying these agents. The most common techniques used in CWAs determination are spectroscopic and chromatographic techniques. Although they possess remarkable robustness, they require prolonged analysis time, experienced personnel, expensive instrumentation and are not fit for on-field applications and fast early alert. Fortunately, electrochemical sensors represent a viable alternative due to their, simple instrumentation, high sensitivity and low cost. The aim of this article is to highlight some important aspects of electrochemical sensing and to present some electrochemical sensors developed for CWAs detection. The future perspectives and challenges in electrochemical sensor development for CWAs detection is also discussed.

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Engineering organophosphate hydrolase for enhanced biocatalytic performance: A review

Cited by 25 publications

References 125 publications

Metal Nanoparticles@Covalent Organic Framework@Enzymes: A Universal Platform for Fabricating a Metal–Enzyme Integrated Nanocatalyst

Metal Nanoparticles@Covalent Organic Framework@Enzymes: A Universal Platform for Fabricating a Metal–Enzyme Integrated Nanocatalyst

Recent Advances, Opportunities, and Challenges in Developing Nucleic Acid Integrated Wearable Biosensors for Expanding the Capabilities of Wearable Technologies in Health Monitoring

Electrochemical Sensors for the Detection of Chemical Warfare Agents: Strategy and Exemples

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