Engineering Pediococcus acidilactici with xylose assimilation pathway for high titer cellulosic l-lactic acid fermentation

Qiu, Zhongyang; Gao, Qinghai; Bao, Jie

doi:10.1016/j.biortech.2017.09.117

Cited by 75 publications

(54 citation statements)

References 27 publications

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“…Several lactic acid bacteria (LAB) such as Lactobacillus pentosus [ 4 ], Lactobacillus brevis [ 15 ], Enterococcus mundtii QU 25 [ 16 ], and Enterococcus faceium QU 50 [ 17 ] have been reported to ferment xylose via the phosphoketolase pathway (hetero-fermentation pathway). Recently, including Lactobacillus strains [ 4 , 15 , 18 ], Bacillus coagulans [ 12 , 19 , 20 , 21 ], and Pediococcus acidilactici [ 22 , 23 ], they have also been reported to produce high-titer LA from lignocellulosic materials. These strains with robust inhibition tolerance were found to be suitable for lignocellulose-derived LA production and were engineered for chemical production because of their thermophilic growth characteristics (except Lactobacillus strains) and strong pentose homofermentative activity.…”

Section: Introductionmentioning

confidence: 99%

High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

Zhang

Jian-guo

et al. 2020

Microorganisms

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Lignocellulose comprised of cellulose and hemicellulose is one of the most abundant renewable feedstocks. Lactic acid bacteria have the ability to ferment sugar derived from lignocellulose. In this study, Pediococcus acidilactici PA204 is a lactic acid bacterium with a high tolerance of temperature and high-efficiency utilization of xylose. We developed a fed-batch simultaneous saccharification and fermentation (SSF) process at 37 °C (pH 6.0) using the 30 FPU (filter paper units)/g cellulase and 20 g/L corn steep powder in a 5 L bioreactor to produce lactic acid (LA). The titer, yield, and productivity of LA produced from 12% (w/w) NaOH-pretreated and washed stover were 92.01 g/L, 0.77 g/g stover, and 1.28 g/L/h, respectively, and those from 15% NaOH-pretreated and washed stover were 104.11 g/L, 0.69 g/g stover, and 1.24 g/L/h, respectively. This study develops a feasible fed-batch SSF process for LA production from corn stover and provides a promising candidate strain for high-titer and -yield lignocellulose-derived LA production.

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Section: Introductionmentioning

confidence: 99%

High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

Zhang

Jian-guo

et al. 2020

Microorganisms

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“…The fermentation supernatant was passed through a 0.22-µm filter and analyzed for glucose levels, using a SBA-40C biosensor (developed by Biology Institute of Shandong Academy of Sciences). Acetic acid were analyzed as described previously [ 23 ]. All experiments were conducted in triplicate; the data were averaged and presented as mean ± standard deviation (SD).…”

Section: Methodsmentioning

confidence: 99%

Optimization of ʟ-ornithine production in recombinant Corynebacterium glutamicum S9114 by cg3035 overexpression and manipulating the central metabolic pathway

Zhang

Wei

et al. 2018

Microb Cell Fact

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Backgroundʟ-Ornithine is an important amino acid with broad applications in pharmaceutical and food industries. Despite lagging ʟ-ornithine productivity and cost reduction, microbial fermentation is a promising route for sustainable ʟ-ornithine production and thus development of robust microbial strains with high stability and productivity is essential.ResultsPreviously, we systematically developed a new strain, SO1 originate from Corynebacterium glutamicum S9114, for ʟ-ornithine production. In this work, overexpression of cg3035 encoding N-acetylglutamate synthase (NAGS) using a plasmid or by inserting a strong Ptac promoter into the chromosome was found to increase ʟ-ornithine production in the engineered C. glutamicum SO1. The genome-based cg3035 modulated strain was further engineered by attenuating the expression of pta and cat, inserting a strong Peftu promoter in the upstream region of glycolytic enzymes such as pfkA, gap, and pyk, and redirecting carbon flux to the pentose phosphate pathway. The final strain with all the exploratory metabolic engineering manipulations produced 32.3 g/L of ʟ-ornithine, a yield of 0.395 g ornithine per g glucose, which was 35.7% higher than that produced by the original strain (23.8 g/L).ConclusionThese results clearly demonstrated that enhancing the expression of NAGS promoted ʟ-ornithine production and provide a promising alternative systematic blueprint for developing ʟ-ornithine-producing C. glutamicum strains.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0940-9) contains supplementary material, which is available to authorized users.

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“…The l-ornithine titer was measured by colorimetry using ninhydrin as described previously [40,41]. An SBA-40C biosensor (developed by Biology Institute of Shandong Academy of Sciences) was employed for glucose analysis in fermentation liquid [42]. To increase the reliability of measurements, samples were collected from three parallel experiments in order to calculate average values and standard deviations.…”

Section: Measurement Of Cell Growth and Metabolites Concentrationmentioning

confidence: 99%

Proteome analysis guided genetic engineering of Corynebacterium glutamicum S9114 for tween 40-triggered improvement in l-ornithine production

et al. 2020

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Background: l-ornithine is a valuable amino acid with a wide range of applications in the pharmaceutical and food industries. However, the production of l-ornithine by fermentation cannot compete with other methods, because of the low titers produced with this technique. Development of fermentation techniques that result in a high yield of l-ornithine and efficient strategies for improving l-ornithine production are essential. Results: This study demonstrates that tween 40, a surfactant promoter of the production of glutamate and arginine, improves l-ornithine production titers in engineered C. glutamicum S9114. The intracellular metabolism under tween 40 triggered fermentation conditions was explored using a quantitative proteomic approach, identifying 48 up-regulated and 132 down-regulated proteins when compared with the control. Numerous proteins were identified as membrane proteins or functional proteins involved in the biosynthesis of the cell wall. Modulation of those genes revealed that the overexpression of CgS9114_09558 and the deletion of CgS9114_13845, CgS9114_02593, and CgS9114_02058 improved the production of l-ornithine in the engineered strain of C. glutamicum Orn8. The final strain with all the exploratory metabolic engineering manipulations produced 25.46 g/L of l-ornithine, and a yield of 0.303 g l-ornithine per g glucose, which was 30.6% higher than that produced by the original strain (19.5 g/L). Conclusion: These results clearly demonstrate the positive effect of tween 40 addition on l-ornithine accumulation. Proteome analysis was performed to examine the impact of tween 40 addition on the physiological changes in C. glutamicum Orn8 and the results showed several promising modulation targets for developing l-ornithine-producing strains.

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Engineering Pediococcus acidilactici with xylose assimilation pathway for high titer cellulosic l-lactic acid fermentation

Cited by 75 publications

References 27 publications

High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

High-Titer Lactic Acid Production by Pediococcus acidilactici PA204 from Corn Stover through Fed-Batch Simultaneous Saccharification and Fermentation

Optimization of ʟ-ornithine production in recombinant Corynebacterium glutamicum S9114 by cg3035 overexpression and manipulating the central metabolic pathway

Proteome analysis guided genetic engineering of Corynebacterium glutamicum S9114 for tween 40-triggered improvement in l-ornithine production

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