Engineering Yarrowia lipolytica to enhance lipid production from lignocellulosic materials

Niehus, Xochitl; Coq, Anne‐Marie Crutz‐Le; Sandoval, Georgina; Nicaud, Jean‐Marc; Ledesma-Amaro, Rodrigo

doi:10.1186/s13068-018-1010-6

Cited by 121 publications

(88 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results obtained with crude xylose from SCB hydrolysate were highly encouraging. The yeast grew robustly (OD 600 : 25.3) on crude xylose despite the presence of AA and furfural at signi cant levels ( Figure 6B), in accordance with previous reports on agave hydrolysates [29]. The resultant SA concentration was 5.6 g/L with a yield of 0.14 g/g, which is similar to those obtained with pure xylose (0.19 g/g).…”

Section: Discussionsupporting

confidence: 89%

Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control

Prabhu

Amaro

Lin

et al. 2020

Preprint

View full text Add to dashboard Cite

Background : Xylose is a most prevalent sugar available in hemicellulose fraction of lignocellulosic biomass (LCB) and of great interest for the green economy. Unfortunately, most of the cell factories cannot inherently metabolize xylose as sole carbon source. Yarrowia lipolytica is a non-conventional yeast to produce industrially important metabolites. The yeast is able to metabolize a large variety of substrates including both hydrophilic and hydrophobic carbon sources. However, Y. lipolytica lacks effective metabolic pathway for xylose uptake and only scarce information is available on utilization of xylose. For the economically feasibility of LCB-based biorefineries, effective utilization of both pentose and hexose sugars is obligatory. Results : In the present study, succinic acid (SA) production from xylose by Y. lipolytica was examined. To this end, Y. lipolytica PSA02004 strain was engineered by overexpressing pentose pathway cassette comprising of xylose reductase ( XR ), xylitol dehydrogenase ( XDH ) and xylulose kinase ( XK ) gene. The recombinant strain exhibited a robust growth on xylose as sole carbon source and produced substantial amount of SA. The inhibition of cell growth and SA formation was observed above 60 g/L xylose concentration. The batch cultivation of recombinant strain in bioreactor resulted in a maximum biomass concentration of 7.3 g/L and SA titer of 11.2 g/L with the yield of 0.19 g/g. Similar results in term of cell growth and SA production were obtained with xylose-rich hydrolysate derived from sugarcane bagasse. The fed-batch fermentation yielded biomass concentration of 11.8 g/L (OD 600 : 56.1) and SA titer of 22.3 g/L with a gradual decrease in pH below 4.0. Acetic acid was obtained as a main byproduct in all the fermentations. Conclusion : The recombinant strain displayed potential for bioconversion of xylose to SA. Further, this study provided a new insight on conversion of lignocellulosic biomass into value-added products. To the best of our knowledge, this is the first study on SA production by Y. lipolytica using xylose as a sole carbon source.

show abstract

Section: Discussionsupporting

confidence: 89%

Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control

Prabhu

Amaro

Lin

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Y. lipolytica is considered to be a promising microbial workhorse for production of high value-added products [39,40]. In this study, we reported the development of quantitative measurement of violacein, based on both HPLC and microplate reader.…”

Section: Resultsmentioning

confidence: 99%

Engineering oleaginous yeast Yarrowia lipolytica for violacein production: extraction, quantitative measurement and culture optimization

Tong

Zhou

Zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

1Violacein is a naturally occurring anticancer therapeutic with deep purple color. 2 Yeast fermentation represents an alternative approach to efficiently manufacturing 3 violacein from inexpensive feedstocks. In this work, we optimized the extraction 4 protocol to improve violacein recovery ratio and purity from yeast culture, including 5 the variations of organic solvents, the choice of mechanical shear stress, incubation 6 time and the use of cell wall-degrading enzymes. We also established the quantitative 7 correlation between HPLC and microplate reader method. We demonstrated that both 8 HPLC and microplate reader are technically equivalent to measure violacein from 9 yeast culture. Furthermore, we optimized the yeast cultivation conditions, including 10 carbon/nitrogen ratio and pH conditions. Our results indicated that ethyl acetate is the 11 best extraction solvent with glass beads grinding the cell pellets, the maximum 12 violacein and deoxyviolacein production was 70.04 mg/L and 5.28 mg/L in shake 13 flasks, respectively. Violacein purity reaches 86.92% at C/N ratio of 60, with addition 14 of 10 g/L CaCO 3 to control the media pH. Taken together, the development of 15 efficient extraction protocol, quantitative correlation between HPLC and microplate 16 reader, and the optimization of culture conditions set a new stage for engineering 17 violacein production in Y. lipolytica. This information should be valuable for us to 18 build a renewable and scalable violacein production platform from the novel host 19 oleaginous yeast species. 20 21

show abstract

“…However, cells seem to prefer the alternative pathway of transketolase as it provides more carbon towards glycolysis and oxidative PPP, where the majority of NADPH is regenerated. The overexpression of phosphoketolase and phosphotransacetylase enzymes in Y. lipolytica resulted in 20-50% increased lipid production (Niehus et al, 2018). Therefore, the upregulation of phosphoketolase could be partially related to the increased accumulation of lipids and carotenoids at the aforementioned phases.…”

Section: Discussionmentioning

confidence: 99%

Xylose Metabolism and the Effect of Oxidative Stress on Lipid and Carotenoid Production in Rhodotorula toruloides: Insights for Future Biorefinery

Pinheiro

Bonturi

Belouah

et al. 2020

Preprint

View full text Add to dashboard Cite

The use of cell factories to convert sugars from lignocellulosic biomass into chemicals in which oleochemicals and food additives, such as carotenoids, is essential for the shift towards sustainable processes. Rhodotorula toruloides is a yeast that naturally metabolises a wide range of substrates, including lignocellulosic hydrolysates, and converts them into lipids and carotenoids. In this study, xylose, the main component of hemicellulose, was used as the sole substrate for R. toruloides, and a detailed physiology characterisation combined with absolute proteomics and genome-scale metabolic models was carried out to understand the regulation of lipid and carotenoid production. To improve these productions, oxidative stress was induced by hydrogen peroxide and light irradiation and further enhanced by adaptive laboratory evolution. Based on the online measurements of growth and CO2 excretion, three distinct growth phases were identified during batch cultivations. The intracellular flux estimations correlated well with the measured protein levels and demonstrated improved NADPH regeneration, phosphoketolase activity and reduced beta-oxidation, correlating with increasing lipid yields. Light irradiation resulted in 70% higher carotenoid and 40% higher lipid yields. The presence of hydrogen peroxide did not affect the carotenoid yield but culminated in the highest lipid yield of 0.65 g/gDCW. The adapted strain showed improved fitness and 2.3-fold higher carotenoid yield than the parental strain. This work presented a holistic view of xylose conversion into microbial oil and carotenoids by R. toruloides for further cost-effective and renewable production of these molecules.

show abstract

Engineering Yarrowia lipolytica to enhance lipid production from lignocellulosic materials

Cited by 121 publications

References 30 publications

Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control

Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control

Engineering oleaginous yeast Yarrowia lipolytica for violacein production: extraction, quantitative measurement and culture optimization

Xylose Metabolism and the Effect of Oxidative Stress on Lipid and Carotenoid Production in Rhodotorula toruloides: Insights for Future Biorefinery

Contact Info

Product

Resources

About