engrailed sequences from four centipede orders: strong sequence conservation, duplications and phylogeny

Bastianello, Alberto; Minelli, Alessandro

doi:10.1007/s00427-001-0193-0

Cited by 9 publications

(6 citation statements)

References 16 publications

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“…Four degenerate primers were designed for nested polymerase chain reaction (PCR) (based on Bastianello and Minelli 2001) by aligning engrailed sequences from a lithobiomorph, four insect species (including Drosophila ), two crustacean species, a millipede, and a chelicerate. Forward primers CDHDEK (TCGAA GAGAAGCGRCCXMGXAC) and enfor1 (GAAGAAAARMGR CCAMGRACGGC) corresponded to DNA sequence at the 5′ end of the engrailed homeobox, whereas reverse primers CDHMAQ (TGGTGTGGTTGTACAAGGCCYTSXGCCAT) and CDHQLM (CAGCCCCTGTGCCATXARXTG) were complementary to sequence immediately 3′ to the engrailed homeobox.…”

Section: Methodsmentioning

confidence: 99%

The pattern of segment formation, as revealed by engrailed expression, in a centipede with a variable number of segments

Kettle

Johnstone

Jowett

et al. 2003

Evolution and Development

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Arthropods vary enormously in segment number, from less than 20 to more than 200. This between-species variation must have originated, in evolution, through divergent selection operating in ancestral arthropod species with variable segment numbers. Although most present-day arthropod species are invariant in this respect, some are variable and so can serve as model systems. Here, we describe a study based on one such species, the coastal geophilomorph centipede Strigamia maritima. We investigate the way in which segments are formed using in situ hybridization to demonstrate the expression pattern of the engrailed gene during embryogenesis. We also analyze segment number data in mother-offspring broods and thereby demonstrate a significant heritable component of the variation. We consider how natural selection might act on this intraspecific developmental variation, and we discuss the similarities and differences in segment formation between the geophilomorphs and their phylogenetic sister-group.

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Section: Methodsmentioning

confidence: 99%

The pattern of segment formation, as revealed by engrailed expression, in a centipede with a variable number of segments

Kettle

Johnstone

Jowett

et al. 2003

Evolution and Development

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show abstract

“…Some of these (Bastianello et al, 2002;Hughes and Kaufman, 2002) involve Hox genes and so do not directly concern us here. However, others (Bastianello and Minelli, 2001;Kettle et al, 2002) focused on the segmentation gene engrailed and so are relevant to the formation of segments and the determination of segment number. The study by Kettle et al (2002) is particularly relevant because it goes beyond gene sequence data to visualization of the pattern of expression of engrailed in geophilomorph embryos.…”

Section: Developmental Bias In Centipede Segment Numbermentioning

confidence: 99%

The interaction between developmental bias and natural selection: from centipede segments to a general hypothesis

Arthur

2002

Heredity

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“…Regier and Shultz (2001) increased their earlier sample (Shultz and Regier 1997) of elongation factor‐1 alpha sequences to 11 chilopods, and also contributed data for the largest subunit of RNA polymerase II for the same species. Engrailed sequences have been explored by Bastianello and Minelli (2001), although data were limited to eight species representing four orders.…”

Section: Introductionmentioning

confidence: 99%

Adding mitochondrial sequence data (16S rRNA and cytochrome c oxidase subunit I) to the phylogeny of centipedes (Myriapoda: Chilopoda): an analysis of morphology and four molecular loci

Edgecombe

Giribet

2004

J Zoological System

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Relationships within Chilopoda (centipedes) are assessed based on 222 morphological characters, complete 18S rRNA sequences for 70 chilopod terminals, the D3 region of 28S rRNA for 65 terminals, 16S rRNA sequences for 54 terminals and cytochrome c oxidase subunit I sequences for 45 terminals. Morphological and molecular data for seven orders of Diplopoda are used to root cladograms for Chilopoda. Analyses use direct character optimization for 15 gap and substitution models. The Pleurostigmophora and Epimorpha s.l. hypotheses are largely stable to parameter variation for the combined data; the latter clade is formalized as the new taxon Phylactometria. The combined data include parameter sets that support either the monophyly of Epimorpha s.str. (¼Scolopendromorpha + Geophilomorpha) or Craterostigmus + Geophilomorpha; the former derives its support from morphology and the nuclear ribosomal genes. Monophyly of Lithobiomorpha and the sister group relationship between Lithobiidae and Henicopidae are stable for morphological and combined data, and are also resolved for the molecular data for 14 of 15 parameter sets. The fundamental split in Scolopendromorpha is between Cryptopidae and Scolopendridae sensu Attems. Blind scolopendromorphs unite as a clade in most molecular and combined analyses, including those that minimize incongruence between data partitions. Geophilomorpha divides into Placodesmata and Adesmata under nine of 15 explored parameter sets.

show abstract

engrailed sequences from four centipede orders: strong sequence conservation, duplications and phylogeny

Cited by 9 publications

References 16 publications

The pattern of segment formation, as revealed by engrailed expression, in a centipede with a variable number of segments

The pattern of segment formation, as revealed by engrailed expression, in a centipede with a variable number of segments

The interaction between developmental bias and natural selection: from centipede segments to a general hypothesis

Adding mitochondrial sequence data (16S rRNA and cytochrome c oxidase subunit I) to the phylogeny of centipedes (Myriapoda: Chilopoda): an analysis of morphology and four molecular loci

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