Enhanced accumulation of flavonolignans in Silybum marianum cultured roots by methyl jasmonate

Elwekeel, Ahlam; Elfishway, Ahlam; AbouZid, Sameh

doi:10.1016/j.phytol.2012.03.010

Cited by 17 publications

(12 citation statements)

References 19 publications

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“…1,37,38 Within silibinin (silybin A and silybin B) and isosilibinin (isosilybin A and isosilybin B), each exists as a pair of trans -diasteroisomers with respect to the relative configuration at positions C-7″ and C-8″ in the 1,4-benzodioxane ring. 26,27,35,36 Silychristin ( 5 ) and isosilychristin ( 6 ) each have coumaran ring systems, or dihydrobenzofuran bicycles, but the position of this bicycle differs by being formed at either the C-4′ and C-5′ positions in silychristin or the C-2′ and C-3′ positions in isosilychristin.…”

Section: Introductionmentioning

confidence: 99%

Mechanistic Study of the Biomimetic Synthesis of Flavonolignan Diastereoisomers in Milk Thistle

et al. 2013

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The mechanism for the biomimetic synthesis of flavonolignan diastereoisomers in milk thistle is proposed to proceed by single-electron oxidation of coniferyl alcohol, subsequent reaction with one of the oxygen atoms of taxifolin's catechol moiety, and finally, further oxidation to form four of the major components of silymarin: silybin A, silybin B, isosilybin A, and isosilybin B. This mechanism is significantly different from a previously proposed process that involves the coupling of two independently formed radicals.

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Section: Introductionmentioning

confidence: 99%

Mechanistic Study of the Biomimetic Synthesis of Flavonolignan Diastereoisomers in Milk Thistle

et al. 2013

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“…were adapted to hairy root cultures (Elwekeel et al 2012 ;Georgiev et al 2012 ;Thimmaraju et al 2003 ) (Table 4.4 ).…”

Section: Plant Transformation -Agrobacteriummentioning

confidence: 99%

In Vitro Plant Cultures as Biofactories

Álvarez

2014

Plant Biotechnology for Health

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In vitro plant cell cultures are defi ned as a culture in aseptic conditions of any part of the plant body in a nutritive media. Cells, differentiated and undifferentiated (calli and suspended cells) tissues, and organs can be maintained in vitro .Some of the advantages of in vitro cultures are their development and maintenance in defi ned conditions (nutrients, light, humidity, temperature), independent from environmental infl uences (pests, diseases, extreme weather, geographical location, etc.), and manipulated by experienced operators in confi ned facilities.Since its development, in vitro plant cell cultures have been an essential tool for studying plant metabolism, and to micropropagate plant species, maintaining their genetic background. Also, they were helpful to analyze and optimize the production of plant secondary metabolites.Secondary metabolites productive processes are conducted, with a different degree of success, in undifferentiated cultures and in cultures of transformed organs. Several strategies were displayed in order to increase yields as manipulation of the plant growth regulator balance, addition of precursors, biotransformation, and immobilization.Also, as plant cells have a different behavior in liquid culture compared with microorganisms and mammal cells, bioreactor design was adapted to develop plant cell cultures.

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“…1). Silymarin synthesis in cell and tissue cultures of S. marianum has been reported (Hasanloo et al, 2008;Khalili et al, 2009;Elwekeel et al, 2012). The treatment of plant cells and tissues with elicitors (i.e.…”

Section: Cytology Of Silybum Marianummentioning

confidence: 99%

“…The treatment of plant cells and tissues with elicitors (i.e. methyl jasmonate) has been a useful strategy to enhance silymarin synthesis in cell and tissue cultures (Sánchez-Sampedro et al, 2005a & b;Elwekeel et al, 2012). Elicitors are molecules that improve the synthesis of plant secondary metabolites.…”

Section: Cytology Of Silybum Marianummentioning

confidence: 99%

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Breeding Objectives and Selection Criteria for Milk Thistle [<i>Silybum marianum</i> (L.) Gaertn.] Improvement

Alemardan

Karkanis

Salehi

2013

Not Bot Hort Agrobot Cluj

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Milk thistle [Silybum marianum (L.) Gaertn.] is an important medicinal-industrial plant. The medicinal compounds of milk thistle are derived from its seeds. The plant is commercially cultivated for the production of silymarin. Cultivation offers the opportunity to optimise achene yield and silymarin content. Furthermore, efforts should be made to develop new cultivars. The main objective of milk thistle breeding is to develop high yielding cultivars with elevated silymarin content. There are few developed cultivars of milk thistle (e.g. Argintiu, Budakalaszi, Szibilla, Khoreslo, Babak Castle, Mirel, Silma and Silyb). Different genotypes of milk thistle have variable amount of silymarin e.g. a 'Royston' genotype is rich in silymarin (6-10%). Further progress can be made by using the genetic diversity available in abundance in different genotypes. The main concern of the breeder should be to increase the yield and silymarin contents of the seeds. Asynchronous flowering and seed shedding are also major problems in milk thistle cultivation. At the time of harvest, the plants have flower heads at all stages of development resulting non-uniform maturation of seeds. Therefore, a breeding effort in milk thistle should be planned to obtain plants with simultaneous flowering and reduced crop losses. The developed cultivar Argintiu is characterised by simultaneous seed maturation in flower heads. The wild populations, as valuable gene pools, could be exploited for the improvement of milk thistle crop.

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Enhanced accumulation of flavonolignans in Silybum marianum cultured roots by methyl jasmonate

Cited by 17 publications

References 19 publications

Mechanistic Study of the Biomimetic Synthesis of Flavonolignan Diastereoisomers in Milk Thistle

Mechanistic Study of the Biomimetic Synthesis of Flavonolignan Diastereoisomers in Milk Thistle

In Vitro Plant Cultures as Biofactories

Breeding Objectives and Selection Criteria for Milk Thistle [<i>Silybum marianum</i> (L.) Gaertn.] Improvement

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