Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris

Choix, Francisco J.; Bashan, Yoav; Mendoza, Alberto Bujardón; de‐Bashan, Luz E.

doi:10.1016/j.jbiotec.2014.02.014

Cited by 47 publications

(15 citation statements)

References 55 publications

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“…In this study cell division of microalgae ceased (data not presented) when microalgal was exposed to a nitrogen starvation environment, leading to accumulation of lipids. This observation is similar to that reported by Leman (1997) and Choix et al (2014).…”

Section: Roles Of Nitrogen and Phosphorussupporting

confidence: 93%

Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation

Shen¹,

Chu²,

Lam

et al. 2015

Water Research

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Section: Roles Of Nitrogen and Phosphorussupporting

confidence: 93%

Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation

Shen¹,

Chu²,

Lam

et al. 2015

Water Research

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“…In previous work, we showed that Chlorella sp. populations greatly benefitted from the association with A. brasilense and Bacillus pumilus and the bacteria in these associations survived in the presence of the microalgae within polymeric beads Choix et al, 2014;Hernandez et al, 2009). Until now, the evidence for metabolic exchange was indirect and circumstantial.…”

Section: Discussionmentioning

confidence: 99%

Establishment of stable synthetic mutualism without co-evolution between microalgae and bacteria demonstrated by mutual transfer of metabolites (NanoSIMS isotopic imaging) and persistent physical association (Fluorescent in situ hybridization)

et al. 2016

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The demonstration of a mutualistic interaction requires evidence of benefits for both partners as well as stability of the association over multiple generations. A synthetic mutualism between the freshwater microalga Chlorella sorokiniana and the soil-derived plant growthpromoting bacterium (PGPB) Azospirillum brasilense was created when both microorganisms were co-immobilized in alginate beads. Using stable isotope enrichment experiments followed by high-resolution secondary ion mass spectrometry (SIMS) imaging of single cells, we demonstrated transfer of carbon and nitrogen compounds between the two partners. Further, using fluorescent in situ hybridization (FISH), mechanical disruption and scanning electron microscopy, we demonstrated the stability of their physical association for a period of 10 days after the aggregated cells were released from the beads. The bacteria significantly enhanced the growth of the microalgae while the microalgae supported growth of the bacteria in a medium where it could not otherwise grow. We propose that this microalga-bacterium association is a true synthetic mutualism independent of co-evolution.

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“…An even higher cell yield can be induced under heterotrophic conditions with D -glucose or Na-acetate as carbon sources (Perez-Garcia et al 2010 ). When the microalgae is growing under less than optimal conditions, co-immobilization with A. brasilense mitigates the effect of these adverse condition on growth and metabolism of the microalgae (de- Bashan and Bashan 2008 ;de-Bashan et al 2008c ;Choix et al 2014 ). This system has not been scaled up for biomass production.…”

Section: Increased Bulk For Animal and Human Feedmentioning

confidence: 99%

Interaction of Azospirillum spp. with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications

de‐Bashan¹,

Hernández²,

Bashan³

2015

Handbook for Azospirillum

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The interaction of the bacteria Azospirillum spp. with photosynthetic, single cell microalgae that are co-immobilized in alginate beads provides a significant shortcut for understanding the interaction of this plant growth-promoting bacteria (PGPB) with plants in general. This interaction is currently relevant for studying physiological, physical, biochemical, and molecular aspects. As an independent subfi eld of Azospirillum research, this interaction has some signifi cant potential biotechnological applications, such as wastewater treatment, production of biofuel (ethanol and biodiesel), increased fertility of eroded soils combined with promoting growth of higher plants, production of pigments, and production of biomass. All of these applications have yet to be scaled up and evaluated for their true practical potential. The Logic Behind Using This Interaction as a Model for Plant-Bacteria InteractionA major obstacle in the study of interactions between Azospirillum spp. and plants is the complexity of the plant. Studies of basic plant-bacterium interactions of Azospirillum spp., done mainly with roots, are diffi cult because there are manyDedication : This chapter is dedicated to the memory of the German/Spanish mycorrhizae researcher Dr. Horst Vierheilig (1964 of CSIC in Spain.

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Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris

Cited by 47 publications

References 55 publications

Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation

Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation

Establishment of stable synthetic mutualism without co-evolution between microalgae and bacteria demonstrated by mutual transfer of metabolites (NanoSIMS isotopic imaging) and persistent physical association (Fluorescent in situ hybridization)

Interaction of Azospirillum spp. with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications

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