Enhanced angiogenesis of gene-activated dermal equivalent for treatment of full thickness incisional wounds in a porcine model

Guo, Rui; Xu, Shiwen; Ma, Lie; Huang, Aibin; Gao, Changyou

doi:10.1016/j.biomaterials.2010.06.013

Cited by 88 publications

(59 citation statements)

References 66 publications

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“…Generally, the average tensile strength of healed cutaneous wounds will be less than that of unwounded skin because of the abnormal collagen organization in scar tissue. In previous studies, plasmid DNA encoding VEGF-165 activated collagen-chitosan scaffolds was used to treat full-thickness incisional (Guo et al, 2010) or burn wounds (Guo et al, 2011) using a two-stage grafting procedure in a porcine model. The tensile strength of healed wounds attained about 70-80% of that of normal skin after 100+ days post-grafting.…”

Section: Discussionmentioning

confidence: 99%

“…When covered with silicone, a scaffold with a thickness of 2 mm can be used as a bilayer dermal substitute to treat full-thickness wounds in a two-stage grafting procedure. The results demonstrated that this bilayer collagen-chitosan-silicone membrane has the ability to induce in situ regeneration of dermis (Guo et al, 2010(Guo et al, , 2011Ma et al, 2007). However, its applicability in one-stage composite grafting for the treatment of full-thickness wounds is unknown.…”

Section: Introductionmentioning

confidence: 93%

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The effect of collagen–chitosan porous scaffold thickness on dermal regeneration in a one-stage grafting procedure

Shi

Wang

et al. 2014

Journal of the Mechanical Behavior of Biomedical Materials

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

The effect of collagen–chitosan porous scaffold thickness on dermal regeneration in a one-stage grafting procedure

Shi

Wang

et al. 2014

Journal of the Mechanical Behavior of Biomedical Materials

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“…TMC was able to condense and protect DNA against degradation by nucleases for further endocytosis and growth factor expression (Thanou et al, 2002). A similar approach on incisional wounds revealed an even higher number of new and mature blood vessels, which was attributed to the more severe injury of surrounding cells and blood vessels found in burn wounds (Guo et al, 2010).…”

Section: Tmc In Wound Healing Applicationsmentioning

confidence: 99%

Chitosan as a starting material for wound healing applications

Patrulea

Ostafe

Borchard

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

487

215

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Abstract:Chitosan and its derivatives have attracted great attention due to their properties beneficial for application to wound healing. The main focus of the present review is to summarize studies involving chitosan and its derivatives, especially N,N,N-trimethylchitosan (TMC), N,O-carboxymethyl-chitosan (CMC) and O-carboxymethyl-N,N,Ntrimethyl-chitosan (CMTMC), used to accelerate wound healing. Moreover, formulation strategies for chitosan and its derivatives, as well as their in vitro, in vivo and clinical applications in wound healing are described.

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“…Firstly, based on our previous results, TMC with a quaternization degree of 38% was used as the vector to condense DNA and form nano-complexes [28]. These complexes are beneficial to mask the strong negative charge of DNA and thereby facilitate uptake across the cell membrane [29].…”

Section: Discussionmentioning

confidence: 99%

Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes

Wang

Guo

et al. 2011

Chin. Sci. Bull.

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A composite construct comprising of a bone mesenchymal stem cell (BMSC) sheet, plasmid DNA, encoding human bone morphogenic protein-2 (hBMP-2), and poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) sponge was designed and employed in the restoration of rat calvarial defects. To improve gene transfection efficiency, a cationic chitosan derivative, N,N,N,-trimethyl chitosan chloride (TMC), was employed as the vector. The TMC/DNA complexes had a transfection efficiency of 13% in rat BMSCs, resulting in heterogeneous hBMP-2 expression in a 10-d culture period in vitro. In vivo culture of the composite constructs was performed by implantation into rat full-thickness calvarial defects, using constructs lacking pDNA-hBMP-2 or BMSC sheets as controls. Significantly higher heterogeneous expression of hBMP-2 was detected in vivo at 2 weeks for the cell sheet/DNA complex/scaffold constructs, compared with the constructs lacking pDNA-hBMP-2 or BMSC sheets. New bone formation was evident as early as 4 weeks in the experimental constructs. At 8 weeks, partial bridging of calvarial defects was observed in the experimental constructs, which was significantly better than the constructs lacking pDNA-hBMP-2 or BMSC sheets. Therefore, the combination of the PLGA/HA scaffold with BMSC sheets and gene therapy vectors is effective at enhancing bone formation.gene therapy, bone regeneration, bone marrow stem cells, poly(lactide-co-glycolide), BMP-2 Citation:Li D, Wang W, Guo R, et al. Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes.

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Enhanced angiogenesis of gene-activated dermal equivalent for treatment of full thickness incisional wounds in a porcine model

Cited by 88 publications

References 66 publications

The effect of collagen–chitosan porous scaffold thickness on dermal regeneration in a one-stage grafting procedure

The effect of collagen–chitosan porous scaffold thickness on dermal regeneration in a one-stage grafting procedure

Chitosan as a starting material for wound healing applications

Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes

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