Enhanced Anti-Skin-Aging Activity of Yeast Extract-Treated Resveratrol Rice DJ526

Abstract

Resveratrol is a powerful antioxidant that defends against oxidative stress in cells but is not found in large quantities in plants. Resveratrol-enriched rice DJ526, which was developed as a functional crop, shows a diverse range of biological activities. Resveratrol production is measured as total resveratrol and its glycoside, piceid, which is mainly found in plant-derived resveratrol. In the present study, elicitation using yeast extract (YE), methyl jasmonate, and jasmonic acid increased resveratrol production in DJ526 rice seeds. DJ526 seeds elicited using 1 g/L (YE1) and 5 g/L yeast extract (YE5) showed enhanced resveratrol production and antioxidant activity. YE5-treated DJ526 seeds showed decreased melanin content by 46.1% and 37.0% compared with the negative control and DJ526 (non-elicitation), respectively. Both YE1 and YE5 efficiently improved the wound-healing activity by reducing the wound gap faster than in untreated cells, with a maximum rate of 60.2% at 24 h and complete closure at 48 h. YE1 and YE5 significantly decreased the levels of proinflammatory cytokine, TNF-α, and enhanced collagen synthesis in inflammatory cells. These findings indicate that YE-treated resveratrol rice DJ526 may improve resveratrol production and could be an active antiaging ingredient for cosmetic and skin therapy applications.

The protective role of resveratrol in diabetic wound healing

“…Its underlying mechanisms might be associated with the extracellular purinergic signaling pathway (Zhu et al, 2022). Otherwise, scientists (Kantayos et al, 2022) found that the pretreatment with yeast extracts (YEs) could enhance the antioxidant activity of resveratrol, and following its antioxidant capacity measurement confirmed that YEs‐pretreated resveratrol effectively increased its antioxidant capacity from 3.9% to 94.3%, thus reducing the wound gap in HaCaT cells, with a maximum rate of 60.2% after 24 h and complete closure after 48 h. To maximize its antioxidant property, resveratrol was incorporated into microparticles (size ranging between 20 and 30 μm) through spray drying to treat diabetic wounds. Eroğlu et al (2015) showed that resveratrol‐loaded hyaluronic acid (HA)‐dipalmitoyl phosphatidylcholine (DPPC) microparticles could increase the cell proliferation of human dermal fibroblast cells, which might be related to increase glutathione (GSH)/oxidized glutathione (GSSG) ratio.…”