Enhanced Anti-Tumor (Anti-Proliferation) Activity of Recombinant Human Interleukin-29 (IL-29) Mutants Using Site-Directed Mutagenesis Method

Lü, Yuan; Li, Liyun; Chen, Wei; Wu, Minchen

doi:10.1007/s12010-015-1804-y

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…Lu et al have recently discovered that IL-29 anti-proliferative effects on cancer can be enhanced by creating mutant IL-29 through site-directed mutagenesis techniques. Human IL-29 mutants have been shown to display enhanced anti-tumor activities against the HCT-8 ileocecal adenocarcinoma line, and the previously discussed SGC-7901 gastric adenocarcinoma line (Lu et al, 2015). These findings indicate that mutant IL-29s may be even stronger immunotherapy treatment prospects when compared to native IL-29.…”

Section: Il-29 In Gastric Cancermentioning

confidence: 99%

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The role of IL-29 in immunity and cancer

Kelm

Zhu

Ding

et al. 2016

Critical Reviews in Oncology/Hematology

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Interleukin-29 (IL-29) is a new member of the recently discovered interferon λ (IFNλ) family. It is produced predominantly by maturing dendritic cells and macrophages. It has been implicated in numerous immunological responses and has shown antiviral activity similar to the Type I interferons, although its target cell population is more limited than the Type I interferons. In recent years, the role of IL-29 in the pathogenesis of various cancers has also been extensively studied. In this review, we will discuss the recent advances of IL-29 in immunological processes and the pathogenesis of various cancer.

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Section: Il-29 In Gastric Cancermentioning

confidence: 99%

“…The previously discussed study by Lu et al, in which IL-29 mutants demonstrated anti-proliferative effects in gastric cell lines, also demonstrated that mutant IL-29 displays enhanced anti-tumor activities against the BEL-7402 hepatoma line (Lu et al, 2015).…”

Section: Il-29 In Hepatocellular Carcinomasmentioning

confidence: 99%

The role of IL-29 in immunity and cancer

Kelm

Zhu

Ding

et al. 2016

Critical Reviews in Oncology/Hematology

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Step-By-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL

Mrázková

Malinovská

Wimmerová

2016

Methods in Molecular Biology

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Site-directed mutagenesis is a powerful technique which is used to understand the basis of interactions between proteins and their binding partners, as well as to modify these interactions. Methods of rational design that are based on detailed knowledge of the structure of a protein of interest are often used for preliminary investigations of the possible outcomes which can result from the practical application of site-directed mutagenesis. Also, random mutagenesis can be used in tandem with site-directed mutagenesis for an examination of amino acid "hotspots."Lectins are sugar-binding proteins which, among other functions, mediate the recognition of host cells by a pathogen and its adhesion to the host cell surface. Hence, lectins and their binding properties are studied and engineered using site-directed mutagenesis.In this chapter, we describe a site-directed mutagenesis method used for investigating the sugar binding pattern of the PA-IIL lectin from the pathogenic bacterium Pseudomonas aeruginosa. Moreover, procedures for the production and purification of PA-IIL mutants are described, and several basic methods for characterizing the mutants are discussed.

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Tanapoxvirus lacking the 15L gene inhibits melanoma cell growth in vitro by inducing interferon-λ1 release

Zhang

Essani

2017

Virus Genes

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Oncolytic viruses (OVs) have emerged as a promising approach for melanoma treatment by causing tumor lysis and inducing immuno-modulatory activities. Tanapoxvirus (TPV), which causes a mild self-limiting disease in humans and contains a large DNA genome, appears as a promising OV candidate. TPV recombinants were generated with the thymidine kinase/66R gene deletion (TPVΔ66R), the 15L gene deletion (TPVΔ15L), or with both the 15L and 66R gene ablation (TPVΔ15LΔ66R). Our previous studies have shown that treatment of TPVΔ15L resulted in significant tumor regression in xenotransplanted human melanoma in nude mice. Here, we demonstrate that an anti-viral activity identified as interferon-λ1 (IFN-λ1) was secreted in a remarkably higher quantity from human lung fibroblast WI-38 and melanoma SK-MEL-3 cells infected with TPVΔ15L. Furthermore, we show that IFN-λ1 exhibits a more pronounced anti-proliferative effect in melanoma cells than IFN-α and IFN-β in vitro. Additional experiments strongly suggest that TPVΔ15L kills melanoma cells partially through inducing IFN-λ1. Taken together, our results demonstrate the immuno-modulatory activities associated with TPVΔ15L and suggest further exploration of TPVΔ15L as a melanoma virotherapy.

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Enhanced Anti-Tumor (Anti-Proliferation) Activity of Recombinant Human Interleukin-29 (IL-29) Mutants Using Site-Directed Mutagenesis Method

Cited by 4 publications

References 19 publications

The role of IL-29 in immunity and cancer

The role of IL-29 in immunity and cancer

Step-By-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL

Tanapoxvirus lacking the 15L gene inhibits melanoma cell growth in vitro by inducing interferon-λ1 release

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