Enhanced Antimicrobial Activity of Nisin‐Loaded Liposomal Nanoparticles against Foodborne Pathogens

Abstract

This study was designed to evaluate the prolonged antimicrobial stability of nisin-loaded liposome (LipoN) nanoparticles against Listeria monocytogenes and Staphylococcus aureus. The sizes of bare liposomes and LipoN were uniformly distributed between 114 and 125 nm. The nanoparticles were homogeneously dispersed in water with less than 0.2 of polydispersity index. The zeta potential value of LipoN was +17.1 mV due to the positive charged nisin, attaining 70% of loading efficiency. The minimum inhibitory concentration of LipoN against L. monocytogenes and S. aureus was 320 international unit/mL. The LipoN significantly enhanced the antimicrobial stability in brain heart infusion agar compared to free nisin. The numbers of L. monocytogenes and S. aureus exposed to LipoN were effectively reduced by more than 6 log colony-forming unit/mL after 48 and 72 h of incubation, respectively. These results provide useful information for the development of antimicrobial delivery system to improve food safety.

Stability and Antimicrobial Activity of Nisin-Loaded Mesoporous Silica Nanoparticles: A Game-Changer in the War against Maleficent Microbes

“…In general, the main aim of the current study is to develop an efficient method for the loading of nisin onto mesoporous silica nanoparticles of various surface chemistries, so as to enhance the antimicrobial agent's stability under various conditions. The obtained results in the current study on the nisin when loaded on the nanoparticles are comparable to those reported in previous studies 17,[39][40][41] . The results obtained in those studies indicate that the loading of nisin onto nanoparticles such as chitosan/alginate 17 , liposomal 41 , solid lipid 40 and carbohydrate nanoparticles 39 improves its antibacterial activity and stability.…”

Strategies employed in the design and optimization of synthetic antimicrobial peptide amphiphiles with enhanced therapeutic potentials

“…Delivery of melittin resulted in more than 3 log reductions in the number of viable E. coli colonies within 60 min of incubation, with activity preserved despite repeated addition of bacterial cells due to the extended release of melittin from the PEG-stabilized lipid disks. Similarly, the encapsulation of nisin in liposomes composed of egg hydrogenated L-α-phosphatidylcholine/cholesterol (8:1, w/w) to form narrowly dispersed nisin-loaded liposomes (LipoNs) of 125 nm in diameter and + 17.1 mV zeta-potential was found to significantly enhance the stability and activity of the AMP in brain heart infusion agar against Listeria monocytogenes and S. aureus as compared to free nisin [154].…”

Food Safety through Natural Antimicrobials

“…Nisin nanoparticles have been tested against L. monocytogenes and S. aureus with good results [302][303][304], as well as bacteriocin nanovesicles [210] or nanoparticles [208,209] against different pathogens. Zou et al [302] evaluated the prolonged antimicrobial stability of liposome nanoparticles loaded with nisin against L. monocytogenes and S. aureus. The MIC of the nanoparticles against both microorganisms was 320 UI/mL, reducing their populations by more than 6 log CFU/mL after 48 and 72 h of incubation, respectively.…”