Enhanced antimicrobial treatment by a clay-based drug nanocarrier conjugated to a guanidine-rich cell penetrating peptide

Abstract

A novel anti-infection therapeutic nano-bioconjugate based on magnetized halloysite nanotubes and a CPP is presented. High levels of bactericidal effects have been obtained with the designed nanocargo in comparison with the individual drugs.

Synergies in antimicrobial treatment by a levofloxacin-loaded halloysite and gold nanoparticles with a conjugation to a cell-penetrating peptide

“…Hence, it would be of high importance to highlight the advantages of the presented lvx@HNT/Au-CPP nano-cargo via true comparisons. Recently, a similar system was reported by Khodabakhshi and Baghersad, 22 which a guanidine-rich cell-penetrating peptide was considered for the conjugation onto the halloysite surfaces. As the first and foremost challenge, it be noticed that the total yield of the peptide synthesis process is quite low because of steric hindrance by the structure of the arginine amino acid.…”

“…For instance, Khodabakhshi and Baghersad used a magnetized system based on the HNTs for the delivery of vancomycin to the bacterial strains. 22 There, it was revealed that great co-localization was obtained for the HNTs and targeted bacteria cells, which resulted in an acceptable cell death ratio. Also, we previously experimented with heat-sensitivity of the HNTs that was exploited for the controlled release of taxotere (a cytotoxic agent) in the ovarian cancer cells.…”

“…According to literature, plasmonic heating of the AuNPs (incorporated into the HNTs) leads the rolled walls to be expanded and the whole structure is opened. 21,22 As a result, the majority of the encapsulated lvx into the pores, and between the layers is immediately released with high control.…”

“…Finally, the obtained lvx@HNT/Au particles were collected by centrifugation (10K RCF, 15 min), washed three times with deionized water, then redispersed in deionized water (10 mL), and dried by using a freeze dryer. 21,22 2.2.5. Synthesis of the CPP sequence.…”

Boosting nitrogen fertilization by a slow releasing nitrate-intercalated biocompatible layered double hydroxide–hydrogel composite loaded withAzospirillum brasilense

“…[Zn–Al–NO 3 − ]–LDH encapsulated with biodegradable alginate beads for enhanced nitrogen fertilization, 23 composites of norfloxacin-intercalated Mg–Al LDH with carboxymethyl cellulose, cross-linked by citric acid for biomedical applications, 24 carboxymethyl cellulose-based nanocomposite hydrogel beads with Cu–Al LDH for bacterial infection treatment 18 and several other layered nanocomposites are reported to have shown controlled release property. 25–28 …”