Enhanced Antioxidant Activity in Mung Bean Seedlings Grown under Slow Clinorotation

Nakajima, Shusaku; Ogawa, Yuichi; Kondo, Naoshi

doi:10.1007/s12217-019-9699-9

Cited by 13 publications

(6 citation statements)

References 38 publications

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“…Findings of this study indicated that microgravity treatment was capable of increasing catalase and peroxidase activity after 3, 7 and 10 days of treatment. Along with this nding, enhanced antioxidant activity under the effect of clinorotation (2 rpm) has been reported previously (Al-Awaida et al 2020; Nakajima et al 2019). Our nding corroborates earlier studies, which reported that microgravity leads to increased level of antioxidant enzymes activity (Chen et al 2015;Lu et al 2009).…”

Section: Discussionsupporting

confidence: 83%

The Effect of Simulated Microgravity On The Antioxidant Capacity And Alkaloid Formation In The Hyoscyamus Niger

Pourhabibian¹,

Iranbakhsh²,

Ebadi³

et al. 2021

Preprint

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Microgravity is one of the most important abiotic stresses in space. In the case of plant exposure to short term microgravity, plants establish strategies to response to these stresses and promote growth and survival. We hypothesized that the simulated microgravity can promote the antioxidant capacity and the formation of secondary metabolites such as tropane alkaloids in the Hyoscyamus niger. Callus induction was conducted by putting hypocotyl segments of H. niger seedlings in solid MS medium supplemented with 1 mg L−1 2,4-D and 1 mg L−1 BAP growth regulators. Then, the sub-cultured calli were placed on a clinostat for 3, 7 and 10 days. We performed Atropine and Scopolamine determination through HPLC. PAL (Phenyle alanine amonalyase) and antioxidant activity were also determined. Gene expression analysis of jasmonic acid (JA), Hyoscyamine 6-beta Hydroxylase (H6H), Putrescine N-methyltransferase (PMT), mitogen-activated protein kinase (MAPK) and ethylene responsive element binding (EREB) was performed using quantitative real time PCR. Findings showed that microgravity had a positive effect on the antioxidant capacity, Atropine and Scopolamine production in the H. niger calli. However, microgravity had a negative effect on the PAL activity. Furthermore, gene expression analysis indicated that microgravity significantly induced gene expression of the H6H, PMT and JA. It was also revealed that callus growth, carbohydrate and protein content increased in response to microgravity treatment. We conclude that microgravity can be considered as a potent factor to induce plant antioxidant activity and tropane alkaloids formation to be applicable in the pharmaceutical and medicinal industries.

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Section: Discussionsupporting

confidence: 83%

The Effect of Simulated Microgravity On The Antioxidant Capacity And Alkaloid Formation In The Hyoscyamus Niger

Pourhabibian¹,

Iranbakhsh²,

Ebadi³

et al. 2021

Preprint

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“…It has been found that many kinds of fruits have strong antioxidant capacity, such as berries, citrus, kiwi fruit, and so on. Lotus root, potato, tomato, spinach, ginger, green pepper, and other vegetables have strong antioxidant capacity [12]. Wine such as whisky, Japanese rice wine, red wine, and Chinese rice wine have strong antioxidant capacity.…”

Section: Analysis Of Antioxidant Principlementioning

confidence: 99%

Antioxidant Activity of Potato Seedlings at Different Storage Temperatures

Wang

Zhang

Liu

et al. 2021

International Journal of Chemical Engineering

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In order to promote the storage and growth of potato seedlings, the antioxidant activities of potato seedlings at different storage temperatures were analyzed. The antioxidant activities of potato seedlings at two different temperatures were compared. In addition, the effects of high temperature and normal temperature on the growth phenotype and antioxidant system physiological indexes of potato seedlings were analyzed by high temperature treatment. According to the significant change characteristics of SOD activity of antioxidant index at different temperatures, the SOD genes of potato were screened and bioinformatics analysis and gene expression analysis were carried out. The results showed that high temperature inhibited the antioxidant activity of potato seedlings and affected the growth of potato seedlings. The growth of potato seedlings can be promoted in medium and low temperature environment. In the future research, the experimental results can be used as the theoretical basis for potato planting.

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“…The samples with the highest amount of phenolic compounds like 6D, 7DM, and 5DM were grown in darkness and two of them (5DM and 7DM) additionally in simulated microgravity conditions, which may suggest that the absence of light and disturbed gravity may be stress factors that influence the increased synthesis of the mentioned compounds in white clover sprouts. Similarly, Nakajima et al (2019) discovered an increased concentration of phenolic compounds in the mung bean seedlings grown in disturbed gravity with the use of clinostat [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

“…According to one of the recent studies [ 8 ], microgravity conditions, a phenomenon described as a state of space with a decreased gravitational acceleration, can enhance the growth of mung bean seedlings, a plant from the Fabaceae family, like clover. Moreover, microgravity caused an increase in the antioxidant activity and the content of phenolic compounds in 4-days-old seedlings of mung bean [ 9 ]. In turn, Wakabayashi et al (2005) observed an increase in the concentration of phenolic acids in 3-days-old wheat seedlings growing in hypergravity conditions [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Pinus pinea seedlings showed higher contents of enzymes involved in seed germination in microgravity conditions, but lower contents in hypergravity conditions, and the results indicate that microgravity can stimulate plant growth [ 11 ]. These results inspired us to explore the issue, especially since, apart from the mentioned work by Nakajima et al (2019), we did not find any results regarding the impact of disturbed gravity on the biological activity of sprouts [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Can Simulated Microgravity and Darkness Conditions Influence the Phytochemical Content and Bioactivity of the Sprouts?—A Preliminary Study on Selected Fabaceae Species

Grudzińska,

Galanty,

Prochownik

et al. 2024

Plants

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Sprouts’ consumption has become popular due to their wide availability, easy cultivation process, and proven biological activity. Moreover, stress factors, such as limited access to light or disturbed gravity during growth, may contribute to the increased activity and the synthesis of bioactive compounds. In this study, for the first time, the examination of the impact of darkness and simulated microgravity conditions on the white clover sprouts from the Fabaceae family was conducted. Among several species, used in the preliminary attempts, only white clover was satisfactory sprouting in the disturbed gravity conditions, and thus was chosen for further examination. A random positioning machine setup was used during the cultivation process to simulate microgravity conditions. Additionally, the sprouts were cultivated in total darkness. Simulated microgravity and/or darkness during the first few days of the sprouts’ growth caused biomass reduction, the increased synthesis of bioactive compounds (isoflavones and phenolics), and changes in the level of abscisic acid and phenylalanine ammonia-lyase. Moreover, it increased the antioxidant properties of the sprouts, while the enhancement of their cytotoxic impact was observed only for androgen-dependent prostate cancer LNCaP cells. To conclude, the presented results are promising in searching for novel functional food candidates and further studies are necessary, directed at other plant families.

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Enhanced Antioxidant Activity in Mung Bean Seedlings Grown under Slow Clinorotation

Cited by 13 publications

References 38 publications

The Effect of Simulated Microgravity On The Antioxidant Capacity And Alkaloid Formation In The Hyoscyamus Niger

The Effect of Simulated Microgravity On The Antioxidant Capacity And Alkaloid Formation In The Hyoscyamus Niger

Antioxidant Activity of Potato Seedlings at Different Storage Temperatures

Can Simulated Microgravity and Darkness Conditions Influence the Phytochemical Content and Bioactivity of the Sprouts?—A Preliminary Study on Selected Fabaceae Species

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