Enhanced articular cartilage regeneration with SIRT1-activated MSCs using gelatin-based hydrogel

Choi, Sung‐Ja; Lee, Kyoung Mi; Ryu, Seung Bae; Park, Yoo Jung; Hwang, Yoon Jung; Baek, Dae-Hyun; Choi, Yoorim; Park, Kwang Hwan; Парк, Ки Донг; Lee, Jin Woo

doi:10.1038/s41419-018-0914-1

Cited by 20 publications

(10 citation statements)

References 59 publications

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“…Choi et al combined BMSCs treated with resveratrol and gelatin-based hydrogels for the treatment of osteochondral defects in rabbit knees. They found that treatment with these cell-laden hydrogels led to the formation of hyaline cartilage-like repair tissue with vast amounts of collagen type II and increased GAG deposition compared to treatment solely with hydrogels or untreated MSC-laden hydrogels [ 84 ]. In addition, Kim et al examined the therapeutic effects of self-assembled peptide (SAP) hydrogels and BMSCs on OA in rats.…”

Section: Resultsmentioning

confidence: 99%

Combinations of Hydrogels and Mesenchymal Stromal Cells (MSCs) for Cartilage Tissue Engineering—A Review of the Literature

Wagenbrenner¹,

Mayer-Wagner²,

Rudert

et al. 2021

Gels

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Cartilage offers limited regenerative capacity. Cell-based approaches have emerged as a promising alternative in the treatment of cartilage defects and osteoarthritis. Due to their easy accessibility, abundancy, and chondrogenic potential mesenchymal stromal cells (MSCs) offer an attractive cell source. MSCs are often combined with natural or synthetic hydrogels providing tunable biocompatibility, biodegradability, and enhanced cell functionality. In this review, we focused on the different advantages and disadvantages of various natural, synthetic, and modified hydrogels. We examined the different combinations of MSC-subpopulations and hydrogels used for cartilage engineering in preclinical and clinical studies and reviewed the effects of added growth factors or gene transfer on chondrogenesis in MSC-laden hydrogels. The aim of this review is to add to the understanding of the disadvantages and advantages of various combinations of MSC-subpopulations, growth factors, gene transfers, and hydrogels in cartilage engineering.

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Section: Resultsmentioning

confidence: 99%

Combinations of Hydrogels and Mesenchymal Stromal Cells (MSCs) for Cartilage Tissue Engineering—A Review of the Literature

Wagenbrenner¹,

Mayer-Wagner²,

Rudert

et al. 2021

Gels

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“…MSCs have been shown to bind well to cartilage biomaterials and bioactive factors and are increasingly used in KOA treatment. Studies have shown that RES treatment significantly promotes the differentiation of MSCs to chondrocytes ( Choi et al, 2018 ). In addition, Lei et al found that co-treatment of RES with transforming growth factor 3 (IGF-3) promoted the differentiation of MSCs attached to chitosan-gelatin scaffolds (CGS) toward chondrocytes antagonized the chondrocyte catabolic response induced by IL-1β stimulation by inhibiting the NF-kB signaling pathway ( Lei et al, 2008 ).…”

Section: New Applications Of Res In Koamentioning

confidence: 99%

Protective Effect of Resveratrol on Knee Osteoarthritis and its Molecular Mechanisms: A Recent Review in Preclinical and Clinical Trials

Yang¹,

Sun²,

Zhang³

2022

Front. Pharmacol.

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Osteoarthritis (OA) is one of the progressing chronic joint associated with by many complex factors such as age, obesity, and trauma. Knee osteoarthritis (KOA) is the most common type of OA. KOA is characterized by articular cartilage destruction and degeneration, synovial inflammation, and abnormal subchondral bone changes. To date, no practical clinical approach has been able to modify the pathological progression of KOA. Drug therapy is limited to pain control and may lead to serious side effects when taken for a long time. Therefore, searching for safer and more reliable treatments has become necessary. Interestingly, more and more research has focused on natural products, and monomeric compounds derived from natural products have received much attention as drug candidates for KOA treatment. Resveratrol (RES), a natural phenolic compound, has various pharmacological and biological activities, including anti-cancer, anti-apoptotic, and anti-decay. Recently, studies on the effects of RES on maintaining the normal homeostasis of chondrocytes in KOA have received increasing attention, which seems to be attributed to the multi-targeted effects of RES on chondrocyte function. This review summarizes preclinical trials, clinical trials, and emerging tissue engineering studies of RES for KOA and discusses the specific mechanisms by which RES alleviates KOA. A better understanding of the pharmacological role of RES in KOA could provide clinical implications for intervention in the development of KOA.

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“…Studies have shown that sirtuin 1 (SIRT1) is associated with the differentiation of MSCs (17,18). SIRT1 is a nicotinamide adenine dinucleotide (NAD + )-dependent lysine deacetylase.…”

Section: Uncarboxylated Osteocalcin Promotes Osteogenesis and Inhibits Adipogenesis Of Mouse Bone Marrow-derived Mesenchymal Stem Cells Vmentioning

confidence: 99%

Uncarboxylated osteocalcin promotes osteogenesis and inhibits adipogenesis of mouse bone marrow‑derived mesenchymal stem cells via the PKA‑AMPK‑SIRT1 axis

Gong

et al. 2021

Exp Ther Med

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Osteoporosis is a bone disease characterized by reduced bone density, thin cortical bone and large gaps in the bone's honeycomb structure, which increases the risk of bone fragility. Uncarboxylated osteocalcin (unOC), a vitamin K-dependent bone protein, is known to regulate carbohydrate and energy metabolism. A previous study demonstrated that unOC promotes the differentiation of mouse bone marrow-derived mesenchymal stem cells (BMSCs) into osteoblasts, but inhibits their differentiation into adipocytes. However, the underlying mechanism remains unknown. The present study showed that unOC regulated the differentiation potential of BMSCs via protein kinase A (PKA)/AMP-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) signaling. SIRT1, a member of the sirtuin family with deacetylation functions, was upregulated by unOC in BMSCs. Transfection analyses with SIRT1 small interfering RNA indicated that the unOC-induced differentiation shift in BMSCs required SIRT1. Examination of SIRT1 downstream targets revealed that unOC regulated the acetylation levels of runt-related transcription factor (RUNX) 2 and peroxisome proliferator-activated receptor γ (PPARγ). Therefore, unOC inhibited adipogenic differentiation by PPARγ acetylation and promoted osteogenic differentiation by RUNX2 deacetylation. Moreover, phosphorylated PKA and AMPK protein levels increased after unOC treatment, which led to the upregulation of SIRT1. Western blot analysis with PKA and AMPK inhibitors indicated that the PKA-AMPK signaling pathway functioned upstream of SIRT1 and positively regulated SIRT1 expression. These findings led us to propose a model in which unOC regulated BMSC osteogenic differentiation through the PKA-AMPK-SIRT1 axis, giving evidence towards the therapeutic potential of unOC in osteoporosis treatment.

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Enhanced articular cartilage regeneration with SIRT1-activated MSCs using gelatin-based hydrogel

Cited by 20 publications

References 59 publications

Combinations of Hydrogels and Mesenchymal Stromal Cells (MSCs) for Cartilage Tissue Engineering—A Review of the Literature

Combinations of Hydrogels and Mesenchymal Stromal Cells (MSCs) for Cartilage Tissue Engineering—A Review of the Literature

Protective Effect of Resveratrol on Knee Osteoarthritis and its Molecular Mechanisms: A Recent Review in Preclinical and Clinical Trials

Uncarboxylated osteocalcin promotes osteogenesis and inhibits adipogenesis of mouse bone marrow‑derived mesenchymal stem cells via the PKA‑AMPK‑SIRT1 axis

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