Combinations of Hydrogels and Mesenchymal Stromal Cells (MSCs) for Cartilage Tissue Engineering—A Review of the Literature

Wagenbrenner, Mike; Mayer-Wagner, Susanne; Rudert, Maximilian; Holzapfel, Boris Michael; Weißenberger, Manuel

doi:10.3390/gels7040217

Cited by 22 publications

(24 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, even though joint replacement serves as an effective remedy for symptomatic end-stage disease including OA, most of the functional outcomes in patients are unsatisfactory and the lifespan of prosthesis is also largely limited [2,9]. Distinguishing from the traditional remedies, cell-based strategies have emerged as an alternative with promising prospective in the treatment of OA and cartilage defects [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…The multifaceted superiorities of MSCs including multidirectional differentiation, high portability property, and low immunogenicity have made themselves ideal seed cells for OA treatment [3]. Meanwhile, MSCs or the derivatives are often encapsulated into natural or synthetic hydrogels, which can function by providing tunable biodegradability, and biocompatibility or enhancing cell vitality and functionality [10].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mesenchymal Stem Cell-based Cytotherapy for Osteoarthritis Management: State of the Art

Zhang¹,

Han²,

Han³

et al. 2023

Arthroplasty - Advanced Techniques and Future Perspectives

View full text Add to dashboard Cite

Osteoarthritis (OA), a principal and challenging disorder of articular cartilage, has been regarded as the most frequent and prevalent chronic disease of degenerative joints, which is caused by multiple factors including aging, trauma, overweight, joint deformity and congenital abnormality, together with the increase in life expectancy. In spite of considerable improvements that have been obtained by conducting multidisciplinary therapies such as surgical procedures and anti-inflammatory drugs, the pathogenesis and efficacy of OA with functional losses and degeneration are still elusively complicated for ascertainment. Mesenchymal stem/stromal cells (MSCs), also termed as multipotent mesenchymal progenitor/precursor cells, skeletal stem cells, or medicinal signaling cells, are heterogeneous cell populations with hematopoietic-supporting and immunomodulatory properties, together with multilineage differentiation property. For decades, investigators have illuminated the application of the advantaged and promising sources with/without remarkable biomaterials for the treatment of recurrent and refractory disorders including OA. In this chapter, we mainly concentrate on the current progress of MSC-based cytotherapy in both preclinical study and clinical practice as well as the promising prospective and critical challenges in the field, which will conformably benefit the administration of OA in future.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cell-based Cytotherapy for Osteoarthritis Management: State of the Art

Zhang¹,

Han²,

Han³

et al. 2023

Arthroplasty - Advanced Techniques and Future Perspectives

View full text Add to dashboard Cite

show abstract

“…They can produce growth factors, chemokines and cytokines and they possess the ability to migrate in the injured sites [ 13 , 14 ]. In vitro and in vivo studies have proved that MSCs with their secretome promote anti-inflammatory effects and contribute to the formation of cartilage tissue [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stromal Cells Laden in Hydrogels for Osteoarthritis Cartilage Regeneration: A Systematic Review from In Vitro Studies to Clinical Applications

Manferdini

Gabusi

Saleh

et al. 2022

Cells

View full text Add to dashboard Cite

This systematic review is focused on the main characteristics of the hydrogels used for embedding the mesenchymal stromal cells (MSCs) in in vitro/ex vivo studies, in vivo OA models and clinical trials for favoring cartilage regeneration in osteoarthritis (OA). PubMED and Embase databases were used to select the papers that were submitted to a public reference manager Rayyan Systematic Review Screening Software. A total of 42 studies were considered eligible: 25 articles concerned in vitro studies, 2 in vitro and ex vivo ones, 5 in vitro and in vivo ones, 8 in vivo ones and 2 clinical trials. Some in vitro studies evidenced a rheological characterization of the hydrogels and description of the crosslinking methods. Only 37.5% of the studies considered at the same time chondrogenic, fibrotic and hypertrophic markers. Ex vivo studies focused on hydrogel adhesion properties and the modification of MSC-laden hydrogels subjected to compression tests. In vivo studies evidenced the effect of cell-laden hydrogels in OA animal models or defined the chondrogenic potentiality of the cells in subcutaneous implantation models. Clinical studies confirmed the positive impact of these treatments on patients with OA. To speed the translation to the clinical use of cell-laden hydrogels, further studies on hydrogel characteristics, injection modalities, chemo-attractant properties and adhesion strength are needed.

show abstract

“…Simultaneously, the inflammatory microenvironment can be regulated by embedding immuno-modulatory elements in the bio-scaffolds ( Glass et al, 2014 ; Tamaddon et al, 2020 ; Tong et al, 2020 ). Among different forms of acellular bio-scaffolds, hydrogels with good biocompatibility, proper degradation rates, and tissue-matched elasticity are widely used ( Liu et al, 2020 ; Wagenbrenner et al, 2021 ; Huang et al, 2022 ). Previously, we prepared an enzymatically crosslinked silk fibroin/pullulan (SF/PL) hydrogel, and proved its feasibility for musculoskeletal tissue engineering ( Li et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Etanercept embedded silk fibroin/pullulan hydrogel enhance cartilage repair in bone marrow stimulation

Song

Wang

Guo

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Background: Bone marrow stimulation (BMS) is the most used operative treatment in repairing cartilage defect clinically, but always results in fibrocartilage formation, which is easily worn out and needs second therapy. In this study, we prepared an Etanercept (Ept) embedded silk fibroin/pullulan hydrogel to enhance the therapeutic efficacy of BMS.Methods: Ept was dissolved in silk fibroin (SF)—tyramine substituted carboxymethylated pullulan (PL) solution and enzyme crosslinked to obtain the Ept contained SF/PL hydrogel. The synergistical effect of SF/PL hydrogel and Ept was verified by rabbit osteochondral defect model. The mechanism of Ept in promoting articular cartilage repair was studied on human osteoarthritic chondrocytes (hOACs) and human bone marrow mesenchymal stromal cells (hBMSCs) in vitro, respectively.Results: At 4 and 8 weeks after implanting the hydrogel into the osteochondral defect of rabbit, histological analysis revealed that the regenerated tissue in Ept + group had higher cellular density with better texture, and the newly formed hyaline cartilage tissue was seamlessly integrated with adjacent native tissue in the Ept + group. In cellular experiments, Ept treatment significantly promoted both gene and protein expression of type II collagen in hOACs, while decreased the protein levels of metalloproteinase (MMP)-13 and a disintegrin and metalloprotease with thrombospondin motifs 5 (ADAMTS5); alcian blue staining, type II collagen and aggrecan stainings showed that addition of Ept significantly reversed the chondrogenesis inhibition effect of tumor necrosis factor alpha (TNF-α) on hBMSCs.Conclusion: BMS could be augmented by Ept embedded hydrogel, potentially by regulating the catabolic and anabolic dynamics in adjacent chondrocytes and enhancement of BMSCs chondrogenesis.

show abstract

Combinations of Hydrogels and Mesenchymal Stromal Cells (MSCs) for Cartilage Tissue Engineering—A Review of the Literature

Cited by 22 publications

References 111 publications

Mesenchymal Stem Cell-based Cytotherapy for Osteoarthritis Management: State of the Art

Mesenchymal Stem Cell-based Cytotherapy for Osteoarthritis Management: State of the Art

Mesenchymal Stromal Cells Laden in Hydrogels for Osteoarthritis Cartilage Regeneration: A Systematic Review from In Vitro Studies to Clinical Applications

Etanercept embedded silk fibroin/pullulan hydrogel enhance cartilage repair in bone marrow stimulation

Contact Info

Product

Resources

About