Enhanced Aspergillus ficuum phytase production in fed-batch and continuous fermentations in the presence of talcum microparticles

Coban, Hasan B.; Demirci, Ali; Turhan, İrfan

doi:10.1007/s00449-015-1384-9

Cited by 24 publications

(16 citation statements)

References 18 publications

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“…For instance, fructofuranosidase and glucoamylase activities were increased to 150 U/mL (3.7-fold) and 190 U/mL (9.5-fold) with the addition of 25 g/L of titanate compared to control, respectively (Driouch et al 2012). Moreover, Coban et al (2015a) performed the production of fungal phytase in microparticle-enhanced fed-batch bioreactor. The highest phytase activities were found as 9.6 U/mL with the addition of 15 g/L of talcum while it was 4.9 U/mL without microparticle.…”

Section: Mannanase Production In Fed-batch Biofilm Reactormentioning

confidence: 99%

“…Moreover, the batch processes have some disadvantages such as limitation of operating time, re-inoculation requirement for each fermentation, time loss due to cleaning and sterilization for each fermentation, substrate or product inhibition as well as low resistance against share force. All those things cause low fermentation efficiency, weakness of cell viability or stability, high recovery cost, low production yield and productivity (Bai et al 2008;Coban et al 2015a;Germec et al 2015). To overcome such problems, different fermentation methods such as immobilization (El-Naggar et al 2006), biofilm formation using support materials (Ercan and Demirci 2015a;Germec et al 2015), and the usage of microparticles such as aluminum oxide (Al 2 O 3 ), magnesium silicate (Talcum, 3MgOÁ4SiO 2 ÁH 2 O), and titanium silicate oxide (Titanate, TiSiO 4 ) have been studied (Coban et al 2015a;Yatmaz et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

et al. 2017

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Mannanases, one of the important enzyme group for industry, are produced by numerous filamentous fungi, especially Aspergillus species with different fermentation methods. The aim of this study was to show the best fermentation method of β-mannanase production for fungal growth in fermenter. Therefore, different fermentation strategies in fed-batch fermentation (suspended, immobilized cell, biofilm and microparticle-enhanced bioreactor) were applied for β-mannanase production from glucose medium (GM) and carob extract medium (CEM) by using recombinant Aspergillus sojae. The highest β-mannanase activities were obtained from microparticle-enhanced bioreactor strategy. It was found to be 347.47 U/mL by adding 10 g/L of AlO to GM and 439.13 U/mL by adding 1 g/L of talcum into CEM. The maximum β-mannanase activities for suspended, immobilization, and biofilm reactor remained at 72.55 U/mL in GM, 148.81 U/mL in CEM, and 194.09 U/mL in GM, respectively. The reason for that is the excessive, and irregular shaped growth and bulk formation, inadequate oxygen transfer or substrate diffusion in bioreactor. Consequently, the enzyme activity was significantly enhanced by addition of microparticles compared to other fed-batch fermentation strategies. Also, repeatable β-mannanase activities were obtained by controlling of the cell morphology by adding microparticle inside the fermenter.

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Section: Mannanase Production In Fed-batch Biofilm Reactormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

et al. 2017

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“…Gonciarz and Bizukojc ( 2014 ) and Gonciarz et al ( 2016 ) studied lovastatin-producing Aspergillus terreus ATCC 20542 and cultivated it in shake flasks with talc microparticles (Gonciarz and Bizukojc 2014 ), and in batch and continuous fed-batch stirred-tank bioreactors (Gonciarz et al 2016 ). Similarly, Coban et al ( 2015a , b ), who studied the enhancement of phytase production by Aspergillus ficuum NRRL 3135 with addition of talc or aluminum oxide, started with shake flasks and then continued in fed-batch and continuous bioreactors. This team later changed their interests to lactic acid production by Rhizopus oryzae NRRL 395 (Coban and Demirci 2016 ).…”

Section: Microparticle-enhanced Cultivation Of Filamentous Fungimentioning

confidence: 99%

“…Coban et al ( 2015a , b ) reported that the addition of microparticles to the A. ficcum NRRL 3135 culture decreased average fungal pellet size and prevented bulk fungal growth. In shake flask cultivations the average fungal pellet radius decreased from 800 µm for control to 500 and 200 µm by increasing the particle concentration to 15 g l −1 of aluminum oxide and talc, respectively.…”

Section: Microparticle-enhanced Cultivation Of Filamentous Fungimentioning

confidence: 99%

“…Lastly, from an industrial point of view, it is important that the concept of morphological engineering was successfully transferred from flasks into bioreactor (Driouch et al 2010a , b , 2012 ; Coban et al 2015b ; Gonciarz et al 2016 ). The relatively low costs of the method (inexpensive microparticles added in low amounts and sodium chloride) is of great relevance for future large scale applications.…”

Section: Summary and Future Studiesmentioning

confidence: 99%

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Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures

Antecka

Bizukojć

Ledakowicz

2016

World J Microbiol Biotechnol

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Morphological engineering techniques have recently gained popularity as they are used for increasing the productivity of a variety of metabolites and enzymes in fungi growing in submerged cultures. Their action is mainly associated with the changes they evoke in fungal morphology. Traditional morphological engineering approaches include manipulation of spore concentration, pH-shifting and mechanical stress exerted by stirring and aeration. As the traditional methods proved to be insufficient, modern techniques such as changes of medium osmolality or addition of mineral microparticles to the media (microparticle-enhanced cultivation, MPEC) were proposed. Despite the fact that this area of knowledge is still being developed, there are a fair amount of scientific articles concerning the cultivations of filamentous fungi with the use of these techniques. It was described that in Ascomycetes fungi both MPEC or change of osmolality successfully led to the change of mycelial morphology, which appeared to be favorable for increased productivity of secondary metabolites and enzymes. There are also limited but very promising reports involving the successful application of MPEC with Basidiomycetes species. Despite the fact that the mineral microparticles behave differently for various microorganisms, being strain and particle specific, the low cost of its application is a great benefit. This paper reviews the application of the modern morphology engineering techniques. The authors critically assess the advantages, shortcomings, and future prospects of their application in the cultivation of fungi.

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Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC)

2018

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The application of microparticle‐enhanced cultivation (MPEC) is an attractive method to control mycelial morphology, and thus enhance the production of metabolites and enzymes in the submerged cultivations of filamentous fungi. Unfortunately, most literature data deals with the spore‐agglomerating species like aspergilli. Therefore, the detailed quantitative study of the morphological evolution of four different fungal species (Aspergillus terreus, Penicillium rubens, Chaetomium globosum, and Mucor racemosus) based on the digital analysis of microscopic images was presented in this paper. In accordance with the current knowledge, these species exhibit different mechanisms of agglomerates formation. The standard submerged shake flask cultivations (as a reference) and MPEC involving 10 μm aluminum oxide microparticles (6 g·L−1) were performed. The morphological parameters, including mean projected area, elongation, roughness, and morphology number were determined for the mycelial objects within the first 24 hr of growth. It occurred that heretofore observed and widely discussed effect of microparticles on fungi, namely the decrease in pellet size, was not observed for the species whose pellet formation mechanism is different from spore agglomeration. In the MPEC, C. globosum developed core‐shell pellets, and M. racemosus, a nonagglomerative species, formed the relatively larger, compared to standard cultures, pellets with distinct cores.

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Enhanced Aspergillus ficuum phytase production in fed-batch and continuous fermentations in the presence of talcum microparticles

Cited by 24 publications

References 18 publications

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures

Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC)

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