Enhanced Attachment and Growth of Human Endothelial Cells Derived from Umbilical Veins on Ammonia Plasma Modified Surfaces of Ptfe and EPTFE Synthetic Vascular Graft Biomaterials

Abstract

Ammonia plasma generated by electrical discharge at low pressure was employed for the surface modification of PTFE and ePTFE. A new chemistry at the plasma treated surfaces is reported. X-ray photoelectron spectroscopy studies showed the incorporation of C-N, C-O, C = O etc functional groups on the plasma treated surfaces. Human endothelial cells derived from umbilical veins (HUEC) were used to seed the plasma treated PTFE and ePTFE surfaces to assess the attachment and growth. Enhanced attachment and growth of HUEC was observed on the plasma treated surfaces. In addition, the performance of these surfaces in this respect was found to be considerably superior to human collagen or human fibronectin or collagen-fibronectin coated PTFE. HUEC attachment and growth on these plasma treated surfaces was further enhanced by immobilizing collagen or fibronectin or collagen-fibronectin. Ammonia plasma treated and untreated ePTFE vascular graft samples were seeded with 3.6 X 10(4) cells/sample. At 24 hrs after seeding, HUEC cell attachment was studied. Although, HUEC attachment on collagen or fibronectin coated ePTFE was improved, but there was no significant difference between the number of cells attached to these surfaces when compared with those adhered to plasma treated ePTFE without collagen or fibronectin coating. Collagen or fibronectin coated plasma treated surfaces showed better performance over their respective controls.

Improving arterial prosthesis neo-endothelialization: Application of a proactive VEGF construct onto PTFE surfaces

“…5, virgin PTFE does not promote the ECs adhesion due to the very low surface energy of this polymer. Conversely, the ammonia plasma-treated PTFE is by far the most efficient surface for supporting cell adhesion, in good agreement with data previously published by the Sipehia's group [29][30][31]. Despite this behavior, it has been shown in many instances that the adhesion of ECs on ammonia plasma-treated PTFE is a non-specific phenomenon, therefore is non-viable in real in vivo situations.…”

Attachment, growth, and activity of rat osteoblasts on polylactide membranes treated with various low‐temperature radiofrequency plasmas

“…This suggestion seems to be supported by the results of recent studies where it was shown that the treatment of polymeric membranes with ammonia plasma promoted adsorption and immobilization of fibronectin and vitronectin, and enhanced the attachment and proliferation of human endothelial cells. 41,45,47 The treatment of the polylactide membranes used in the present study with sulphur-containing plasma had a negative effect on cell parameters. This may be due to the fact that incorporation of the OSH groups into the polylactide membranes makes them resistant to the nonspecific adsorption of proteins, which in turn, diminishes the attachment and proliferation of cells in culture.…”

Low Pressure Radio Frequency Ammonia Plasma Surface Modification on Poly(ethylene terephthalate) Films and Fibers: Effect of the Polymer Forming Process

“…We therefore sought to compare the effect of plasma treatments on the surface modification of PET fibres and PET films, with emphasis on ammonia plasma treatments that promote the formation of surface amino groups which are recognised as improving cell adhesion and, [24][25][26] more importantly, may potentially also be used for further biomolecule conjugation [27,28]. To this end, we examined the dependence between PET structure geometry and plasma treatment duration.…”