Enhanced Bio-hydrogen Production from Protein Wastewater by Altering Protein Structure and Amino Acids Acidification Type

Xiao, Naidong; Chen, Yinguang; Chen, Aihui; Feng, Leiyu

doi:10.1038/srep03992

Cited by 44 publications

(8 citation statements)

References 33 publications

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“…In another study, Choi and Ahn [141] observed that during the anaerobic conversion of sucrose and piggery waste, butyrate was the main by-product when the pH was maintained at 8.9. High pH values have been shown to enhance the digestibility of feedstocks and the ability to inhibit unwanted microbial communities, which ultimately results in increased VFAs production [142][143][144]. Overall, these findings essentially show that although the production of VFAs is highly dependent on the operational pH, the type of feedstocks used during the process also affects the composition of these by-products.…”

Section: Medium Aciditymentioning

confidence: 83%

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Sekoai

Ghimire

Ezeokoli

et al. 2021

Renewable and Sustainable Energy Reviews

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In recent years, much attention has been directed towards the integration of dark fermentation process into a biorefinery concept to enhance the energetic gains, thereby improving the competitiveness of this process. The volatile fatty acids (VFAs) from dark fermentative H 2 -producing processes serve as precursors for the microbial synthesis of a broad spectrum of biotechnologically-important products such as biofuels and biocommodities. These products are desirable substrates for secondary bioprocesses due to their biodegradable nature and affordability. This short review discusses the use of acidogenic-derived VFAs in the production of value-added compounds such as polyhydroxyalkanoates (PHAs) alongside the microbial-based fuels (hydrogen, biogas, and electricity), and other valuable compounds (succinic acid, citric acid, and butanol). The review also highlights the strategies that have been used to enhance the extraction of VFAs from acidogenic effluents and other related waste streams. The application of novel enhancement techniques such as nanoparticles during VFAs recovery is also discussed in this work. Furthermore, the work highlights some of the recent advances in dark fermentationbased biorefinery, particularly the development of pilot-scale processes. Finally, the review provides some suggestions on the advancement of dark fermentation-based biorefineries using VFAs that are derived from acidogenic processes.

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Section: Medium Aciditymentioning

confidence: 83%

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Sekoai

Ghimire

Ezeokoli

et al. 2021

Renewable and Sustainable Energy Reviews

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“…Protease was proved to be effective to decompose protein structure with peptide links being broken during hydrolysis (Han et al, 2016b). The hydrolysate of proteins, which contain several FANs, could be broken down further into organic acids and ammonia via deaminase secreted by fermentative bacteria (Xiao et al, 2014). Therefore, using protease as an additive improves hydrolysis rate and enhances methane production (Moon and Song, 2011).…”

Section: Specific Role Of Enzymes For Different Fw Componentsmentioning

confidence: 99%

Improving the stability and efficiency of anaerobic digestion of food waste using additives: A critical review

Liu

et al. 2018

Journal of Cleaner Production

228

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Anaerobic digestion is an effective technology to treat food waste, with methane production as renewable bioenergy. However, there are two key problems in the practical application, i.e., poor system stability and low reactor efficiency. In this paper, additives used in anaerobic digestion of food waste were systematically reviewed in view of system stability and reactor efficiency. Enzymes showed excellent property in food waste pre-hydrolysis stage with almost all macromolecular matters being rapidly resolved. Fungi fermentation process to produce hydrolytic enzymes, can be regarded as a promising and low-cost way to realize rate-limiting step elimination. It can be also concluded that adding neutralizers, buffer chemicals and some other materials is effective to maintain the pH level for practical application. Trace metals in food waste are not enough but needed for methanogens activation in long term and high loading rate operation. In addition, direct interspecies electron transfer could be much helpful for intermediate refractory organic acids degradation and methanogenesis promotion with additives of conductive materials, which is also discussed and should be studied further in anaerobic digestion of food waste. Based on literature review, a new concept is proposed for further study, suggesting that after being well liquefied with enzyme pre-hydrolysis, food waste could be co-digested with landfill leachate in a high-rate anaerobic reactor stably, resulting in a high bioenergy recovery efficiency.

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“…However, naturally folded proteins are not susceptible to protease cleavage (Halabi et al, 2009), and because protein hydrolysis is the rate-limiting step, it retards HAc generation from WAS. Recently, protein hydrolysis has been found to be accelerated via manipulation of protein native conformation (Xiao et al, 2014); nevertheless, the process inhibited homoacetogenesis, emphasizing the need for enhanced hydrogen production from protein-containing wastewater.…”

Section: Introductionmentioning

confidence: 99%

Augmentation of protein-derived acetic acid production by heat-alkaline-induced changes in protein structure and conformation

Wang

Liu

et al. 2016

Water Research

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a b s t r a c tWaste-derived acetic acid (HAc) is an attractive feedstock for microbe-mediated biofuel production. However, fermentative conversion of HAc from waste-activated sludge (WAS) has low yield because of the high concentration of proteins not readily utilizable by microorganisms without prior hydrolysis. We investigated a combined technology for HAc augmentation during sludge protein fermentation. The maximal HAc yield increased over two-fold, reaching 0.502 ± 0.021 g/g protein (0.36 ± 0.01 g COD/g COD, 52% of the total volatile fatty acids) when synthetic sludge protein was heated at 120 C for 30 min, treated at pH 12 for 24 h, and fermented at pH 9 for 72 h. Comprehensive analysis illustrated that the heat-alkaline pretreatment significantly induced protein fragmentation, simultaneously increasing the efficiency of protein biohydrolysis (from 35.5% to 85.9%) by inducing conformational changes indicative of protein unfolding. Consequently, the native a-helix content was decreased from 67.3% to 32.5% by conversion to an unordered shape, whose content increased from 27.5% to 45.5%; disulfide bonds were cleaved, whereas the main SeS stretching pattern was altered from gauche-gauche-gauche to gauchegauche-trans, consequently causing increased protein susceptibility to proteolytic hydrolysis (76.3% vs. 47.0%). Economic analysis indicated that anaerobic fermentation with appropriate heat-alkaline pretreatment is a cost-effective approach for waste conversion to energy sources such as HAc.

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Enhanced Bio-hydrogen Production from Protein Wastewater by Altering Protein Structure and Amino Acids Acidification Type

Cited by 44 publications

References 33 publications

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept

Improving the stability and efficiency of anaerobic digestion of food waste using additives: A critical review

Augmentation of protein-derived acetic acid production by heat-alkaline-induced changes in protein structure and conformation

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