Enhanced bioactivity and osteoinductivity of carboxymethyl chitosan/nanohydroxyapatite/graphene oxide nanocomposites

Zhang, Yu; Xiao, Caiwen; Huang, Yazhuo; Chen, Mingjiao; Wei, Wei; Yang, Xiaoxuan; Zhou, Huifang; Bi, Xiaoping; Liu, Lu; Ruan, Jing; Fan, Xianqun

doi:10.1039/c8ra00383a

Cited by 36 publications

(33 citation statements)

References 71 publications

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“…Table 2 shows the applications of common chitosan derivatives in bone tissue engineering. Increased pore size and mechanical properties promote osteoblast differentiation [61] Trimethyl chitosan N,N,N-trimethyl chitosan-heparin polyelectrolyte multilayer Bionic periosteum Good cell compatibility and support osteoblast differentiation [142] Hydroxypropyltrimethylammonium chloride chitosan Alginate/HACC/oyster shell powder Preparation bracket Improve mechanical properties and enhance stent surface area [143] CMCS is a commonly used in bone tissue engineering [137][138][139][140][141]. In addition to the applications shown in Table 2, CMCS can also be used to make nanofiber scaffolds [144].…”

Section: Bone Tissue Engineering Materialsmentioning

confidence: 99%

Chitosan Derivatives and Their Application in Biomedicine

Wang

Meng

et al. 2020

IJMS

654

326

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Chitosan is a product of the deacetylation of chitin, which is widely found in nature. Chitosan is insoluble in water and most organic solvents, which seriously limits both its application scope and applicable fields. However, chitosan contains active functional groups that are liable to chemical reactions; thus, chitosan derivatives can be obtained through the chemical modification of chitosan. The modification of chitosan has been an important aspect of chitosan research, showing a better solubility, pH-sensitive targeting, an increased number of delivery systems, etc. This review summarizes the modification of chitosan by acylation, carboxylation, alkylation, and quaternization in order to improve the water solubility, pH sensitivity, and the targeting of chitosan derivatives. The applications of chitosan derivatives in the antibacterial, sustained slowly release, targeting, and delivery system fields are also described. Chitosan derivatives will have a large impact and show potential in biomedicine for the development of drugs in future.

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Section: Bone Tissue Engineering Materialsmentioning

confidence: 99%

Chitosan Derivatives and Their Application in Biomedicine

Wang

Meng

et al. 2020

IJMS

654

326

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“…Stem cells from different sources could be used as progenitor cells in the process of bone formation, so their recruitment and osteogenic differentiation were key factors for effective bone regeneration. GDs were proved to have the ability of osteogenic induction, [144][145][146][147][148][149][150][151][152] as shown in Table 4. In this subsection, the signaling pathways involved in GDs-induced bone regeneration were discussed, including which signaling pathways of osteogenic differentiation of stem cells from different sources were affected by GDs and the effect of GDsrelated parameters on the signaling pathway.…”

Section: Mechanism Of Gds-induced Bone Regeneration Possible Signalinmentioning

confidence: 99%

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Du¹,

Wang

Zhang³

et al. 2020

IJN

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During continuous innovation in the preparation, characterization and application of various bone repair materials for several decades, nanomaterials have exhibited many unique advantages. As a kind of representative two-dimensional nanomaterials, graphene and its derivatives (GDs) such as graphene oxide and reduced graphene oxide have shown promising potential for the application in bone repair based on their excellent mechanical properties, electrical conductivity, large specific surface area (SSA) and atomic structure stability. Herein, we reviewed the updated application of them in bone repair in order to present, as comprehensively, as possible, their specific advantages, challenges and current solutions. Firstly, how their advantages have been utilized in bone repair materials with improved bone formation ability was discussed. Especially, the effects of further functionalization or modification were emphasized. Then, the signaling pathways involved in GDs-induced osteogenic differentiation of stem cells and immunomodulatory mechanism of GDs-induced bone regeneration were discussed. On the other hand, their applications as contrast agents in the field of bone repair were summarized. In addition, we also reviewed the progress and related principles of the effects of GDs parameters on cytotoxicity and residues. At last, the future research was prospected.

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“…In this paper, we found that the expression level of adhesive protein E‐cadherin in the annealed RGO substrate is lower than that in the unannealed substrate. Surface property can induce changes in relevant intracellular signaling pathways by modulating the expression of different adhesion proteins . Therefore, we speculate that changes in surface properties caused by different oxygen‐containing groups first changed the expression of some adhesion proteins in ESC, leading to the changes in related intracellular pathways, and ultimately, the ability of ESC to adhere, proliferate, and self‐renew.…”

Section: Discussionmentioning

confidence: 95%

“…[28][29][30]36] It has been reported that surface property may promote cell adhesion by accelerating the expression of vinculin and fibronectin. [37,38] Therefore, we speculate that changes in surface properties Adv. Surface property can induce changes in relevant intracellular signaling pathways by modulating the expression of different adhesion proteins.…”

Section: Discussionmentioning

confidence: 99%

Structurally Tunable Reduced Graphene Oxide Substrate Maintains Mouse Embryonic Stem Cell Pluripotency

et al. 2019

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Culturing embryonic stem cells (ESCs) in vitro usually requires animal‐derived trophoblast cells, which may cause pathogenic and immune reactions; moreover, the poor repeatability between batches hinders the clinical application of ESCs. Therefore, it is essential to synthesize a xenogeneic‐free and chemically well‐defined biomaterial substrate for maintaining ESC pluripotency. Herein, the effects of structurally tunable reduced graphene oxide (RGO) substrates with different physicochemical properties on ESC pluripotency are studied. Colony formation and CCK‐8 assays show that the RGO substrate with an average 30 µm pore size promotes cell survival and proliferation. The unannealed RGO substrate promotes ESC proliferation significantly better than the annealed substrate due to the interfacial hydrophilic groups. The RGO substrate can also maintain ESC for a long time. Additionally, immunofluorescence staining shows that ESCs cultured on an RGO substrate highly express E‐cadherin and β‐catenin, whereas after being modified by Dickkopf‐related protein 1, the RGO substrate is unable to sustain ESC pluripotency. Furthermore, the cell line that interferes with E‐cadherin is also unable to maintain pluripotency. These results confirm that the RGO substrate maintains ESC pluripotency by promoting E‐cadherin‐mediated cell–cell interaction and Wnt signaling.

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Enhanced bioactivity and osteoinductivity of carboxymethyl chitosan/nanohydroxyapatite/graphene oxide nanocomposites

Abstract: The CMC/nHA/GO scaffold with the surface chemistry and roughness dual effects and the release of phosphate and calcium ions synergistically assist the mineralization and facilitate the bone regeneration.

Cited by 36 publications

References 71 publications

Chitosan Derivatives and Their Application in Biomedicine

Chitosan Derivatives and Their Application in Biomedicine

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Structurally Tunable Reduced Graphene Oxide Substrate Maintains Mouse Embryonic Stem Cell Pluripotency

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