Enhanced bioethanol production from different sugarcane bagasse cultivars using co-culture of Saccharomyces cerevisiae and Scheffersomyces (Pichia) stipitis

Santosh, Ingle; Ashtavinayak, Paradh; Dudhane, Amol; Patil, Sanjay

doi:10.1016/j.jece.2017.05.045

Cited by 29 publications

(10 citation statements)

References 38 publications

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“…At the end of the fermentation, biomass grew very slightly, and slow ethanol consumption was observed ( Figure 2B). Ethanol consumption after sugar exhaustion in simultaneous co-culture has already been reported in the literature [52,53].…”

Section: Erlenmeyer Flask Co-culture Assaysmentioning

confidence: 74%

Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

et al. 2020

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Second-generation bioethanol production's main bottleneck is the need for a costly and technically difficult pretreatment due to the recalcitrance of lignocellulosic biomass (LCB). Chemical pulping can be considered as a LCB pretreatment since it removes lignin and targets hemicelluloses to some extent. Chemical pulps could be used to produce ethanol. The present study aimed to investigate the batch ethanol production from unbleached Kraft pulp of Eucalyptus globulus by separate hydrolysis and fermentation (SHF). Enzymatic hydrolysis of the pulp resulted in a glucose yield of 96.1 ± 3.6% and a xylose yield of 94.0 ± 7.1%. In an Erlenmeyer flask, fermentation of the hydrolysate using Saccharomyces cerevisiae showed better results than Scheffersomyces stipitis. At both the Erlenmeyer flask and bioreactor scale, co-cultures of S. cerevisiae and S. stipitis did not show significant improvements in the fermentation performance. The best result was provided by S. cerevisiae alone in a bioreactor, which fermented the Kraft pulp hydrolysate with an ethanol yield of 0.433 g·g −1 and a volumetric ethanol productivity of 0.733 g·L −1 ·h −1 , and a maximum ethanol concentration of 19.24 g·L −1 was attained. Bioethanol production using the SHF of unbleached Kraft pulp of E. globulus provides a high yield and productivity.Energies 2020, 13, 744 2 of 15 sustainability, has a low and stable price, and practically does not demand extra land [6,7]. There are some facilities producing 2G bioethanol on a commercial scale. However, large-scale production still faces some technological barriers that must be overcome in order to achieve a cost-competitive production [8]. Due to the recalcitrance of LCB, a costly pretreatment step is required, which is the main technological bottleneck of 2G bioethanol production. The release of enzymatic and fermentation inhibitors during pretreatment is another limitation [9].Pulp and paper mills have the infrastructures and logistics to handle LCB, and chemical mills employ technology required for LCB fractionation and conversion [10]. Bioethanol has been produced from different feedstocks such as Kraft pulp, spent sulfite liquor, and pulp and paper sludge [11]. Chemical pulping processes can be considered as a LCB pretreatment since they promote delignification and target hemicelluloses to some degree [12]. Chemical pulping represents about 77% of the virgin pulps produced globally, and more than 95% of these chemical pulps are Kraft pulps [13]. These pulps are produced by Kraft pulping involving the reaction of white liquor, i.e., an alkaline aqueous solution of sodium hydroxide and sodium sulfide with a pH of 14, with lignin at high temperature (150-170 • C). This reaction promotes lignin breakdown and degradation with the release of phenolic fragments, removing almost 90% of the lignin from the wood. Kraft pulping also leads to hemicelluloses and some cellulose loss and decreases the degree of polymerization of cellulose [14]. The utilization of Kraft pulping as a pretreatment method for LCB has ...

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Section: Erlenmeyer Flask Co-culture Assaysmentioning

confidence: 74%

Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

et al. 2020

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“…In parallel hexose and pentose sugar utilization, the lower utilization of pentoses by pentose-fermenting yeast strains presents a major problem. These strains prefer glucose over xylose as a source of carbon, and use glucose in the initial phase of fermentation [5]. For these reasons, we inoculated the fermentation medium with the pentose-fermenting strain after 24 h, once most of the hexoses had been utilized.…”

Section: Ethanol Fermentation Of Sugar Beet Pulp-based Hydrolyzatesmentioning

confidence: 99%

Integrated Bioethanol Fermentation/Anaerobic Digestion for Valorization of Sugar Beet Pulp

et al. 2017

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Large amounts of waste biomass are generated in sugar factories from the processing of sugar beets. After diffusion with hot water to draw the sugar from the beet pieces, a wet material remains called pulp. In this study, waste sugar beet pulp biomass was enzymatically depolymerized, and the obtained hydrolyzates were subjected to fermentation processes. Bioethanol, biomethane, and biohydrogen were produced directly from the substrate or in combined mode. Stillage, a distillery by-product, was used as a feedstock for anaerobic digestion. During biosynthesis of ethanol, most of the carbohydrates released from the sugar beet pulp were utilized by a co-culture of Saccharomyces cerevisiae Ethanol Red, and Scheffersomyces stipitis LOCK0047 giving 12.6 g/L of ethanol. Stillage containing unfermented sugars (mainly arabinose, galactose and raffinose) was found to be a good substrate for methane production (444 dm 3 CH 4 /kg volatile solids (VS)). Better results were achieved with this medium than with enzymatic saccharified biomass. Thermal pre-treatment and adjusting the pH of the inoculum resulted in higher hydrogen production. The largest (p < 0.05) hydrogen yield (252 dm 3 H 2 /kg VS) was achieved with sugar beet stillage (SBS). In contrast, without pre-treatment the same medium yielded 35 dm 3 H 2 /kg VS. However, dark fermentation of biohydrogen was more efficient when sugar beet pulp hydrolyzate was used.

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“…Acid pretreatment is widely used and efficiently removes hemicellulose, where the most common acid for pretreatment is sulfuric acid [20][21][22]. Other acids can also be used, including hydrochloric acid [20], nitric acid [23] and phosphoric acid [24]. However, some released byproducts such as furfural and hydroxymethylfurfural, derived from sugars acid hydrolysis, can decrease fermentation performance.…”

Section: Introductionmentioning

confidence: 99%

Brachiaria brizantha Grass as a Feedstock for Ethanol Production

Rodrigues

Almeida

Maitan-Alfenas

et al. 2021

Braz. arch. biol. technol.

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Different lignocellulosic biomasses are found worldwide and each country has its own important industrial crop that can be converted into high-value products, such as ethanol. Therefore, evaluation of new biomasses to be used in biorefineries is important to decrease the dependence on non-renewable resources and to guarantee sustainable development. This work evaluated Brachiaria brizantha, a grass commonly used as animal forage, and the standard biomass for 2G-ethanol, sugarcane bagasse. The chemical compositions of both biomasses were determined and different times and temperature of acid pretreatment were tested. Morphological analysis via scanning electron microscopy showed more deconstructed fibers after harsher biomass pretreatments. Simultaneous saccharification and fermentation of pretreated Brachiaria brizantha presented higher efficiency than when using sugarcane bagasse as the carbon source. A biomass conversion of 46 % was achieved when Brachiaria brizantha grass was pretreated with 2% sulfuric acid for 60 minutes. Moreover, fermentation was not impaired by the inhibitors furfural and hydroxymethylfurfural. It was concluded that Brachiaria brizantha is a promising biomass for ethanol production.

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Enhanced bioethanol production from different sugarcane bagasse cultivars using co-culture of Saccharomyces cerevisiae and Scheffersomyces (Pichia) stipitis

Cited by 29 publications

References 38 publications

Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption

Integrated Bioethanol Fermentation/Anaerobic Digestion for Valorization of Sugar Beet Pulp

Brachiaria brizantha Grass as a Feedstock for Ethanol Production

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