Enhanced bone formation of calvarial bone defects by low-intensity pulsed ultrasound and recombinant human bone morphogenetic protein-9: a preliminary experimental study in rats

Imafuji, Takatomo; Shirakata, Yoshinori; Shinohara, Yukiya; Nakamura, Toshiaki; Noguchi, Kazuyuki

doi:10.1007/s00784-021-03897-6

Cited by 4 publications

(7 citation statements)

References 49 publications

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“…In vivo , it has also been reported that the co-stimulation with BMP2 and LIPUS enhanced bone formation ( 28 , 29 ). In addition, we recently reported that that LIPUS promoted BMP9-induced bone formation in a rat calvarial bone defect ( 17 ). Our current finding may provide promising support for utilizing DFATs with co-treatment of BMP9 and LIPUS for effective bone tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

“…These reports suggest that DFATs may be a promising cell source for bone tissue engineering. Imafuji et al ( 17 ) recently reported combination of BMP9 and LIPUS resulted in a higher bone formation compared to the carrier/collagen sponge only-group in rat calvarial bone defects. Taken together, in addition to combination of DFATs and BMP9 with an appropriate scaffold, LIPUS stimulation may promote bone formation, suggesting a novel strategy for bone tissue engineering in treating large bone defects.…”

Section: Discussionmentioning

confidence: 99%

“…A previous study showed that osteoblastic differen-tiation of DFATs was significantly induced by BMP9 and co-stimulation with additional agents such as FK506 ( 16 ). Furthermore, combination of BMP9 and LIPUS resulted in higher bone formation compared to the carrier/collagen sponge only-group in rat calvarial bone defects, in vivo ( 17 ). However, the combined effect of BMP9 and LIPUS on osteoblastic differentiation of DFATs has not been stu-died, so far.…”

Section: Introductionmentioning

confidence: 99%

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Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells

2023

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Dedifferentiated fat cells (DFATs) isolated from mature adipocytes have a multilineage differentiation capacity similar to mesenchymal stem cells and are considered as promising source of cells for tissue engineering. Bone morphogenetic protein 9 (BMP9) and low-intensity pulsed ultrasound (LIPUS) have been reported to stimulate bone formation both in vitro and in vivo . However, the combined effect of BMP9 and LIPUS on osteoblastic differentiation of DFATs has not been studied. After preparing DFATs from mature adipose tissue from rats, DFATs were treated with different doses of BMP9 and/or LIPUS. The effects on osteoblastic differentiation were assessed by changes in alkaline phosphatase (ALP) activity, mineralization/calcium deposition, and expression of bone related genes; Runx2, osterix, osteo-pontin. No significant differences for ALP activity, mineralization deposition, as well as expression for bone related genes were observed by LIPUS treatment alone while treatment with BMP9 induced osteoblastic differentiation of DFATs in a dose dependent manner. Further, co-treatment with BMP9 and LIPUS significantly increased osteoblastic differentiation of DFATs compared to those treated with BMP9 alone. In addition, upregulation for BMP9-receptor genes was observed by LIPUS treatment. Indomethacin, an inhibitor of prostaglandin synthesis, significantly inhibited the synergistic effect of BMP9 and LIPUS co-stimulation on osteoblastic differentiation of DFATs. LIPUS promotes BMP9 induced osteoblastic differentiation of DFATs in vitro and prostaglandins may be involved in this mechanism.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells

2023

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“…4 REVIEW including oral maxillofacial procedures and spinal fusion, according to many reports. 12 Treatment with BMP-2 may be more effective at promoting healing. The BMP-2 signaling cascade has been shown in many animal models to start early in the early phase of bone healing, generating an inflammatory response and periosteal activation.…”

Section: Bmp-2 Involvement In Osteogenesismentioning

confidence: 99%

The role of Bone Morphogenetic Protein-2 in craniofacial osteogenesis: A literature review

Sanjaya

Maliawan

2022

Intisari Sains Medis

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Bone Morphogenetic Protein (BMP) is a type of growth factor that belongs to the Transforming Growth Factor Beta (TGF-B) group. Bone Morphogenetic Protein-2 (BMP-2) is recognized as one of the first commercially available osteoblast inductors. When new bone is needed, mesenchymal stem cells can develop into osteoblasts, which then implant as osteocytes in the bone, providing additional structure and support. The principal agent that differentiates stem cells into osteoblasts is BMP-2. BMP-2 will be released into the bone matrix or serum in performing their job. BMP-2 activates the SMAD1/5/8 signal via mediating the physiological action of type I and II serine/threoninekine receptors. The active SMAD protein will form a complex with the SMAD4 protein, translocate DNA to the nucleus, and bind to particular genes like Dlx-2/5, Osx, and transcription. According to a recent study, BMP-2 boosts osteogenic growth. BMPs are effective in the treatment of craniofacial defects in current research. The subjects of this paper are the molecular mechanism of BMPs and their involvement in the clinical application of craniofacial deformity patients.

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“…Available studies regarding LIPUS application on rat calvarial defects are not plentiful, and a small number of published studies report a positive effect of LIPUS on calvarial defect healing [38][39][40][41]. Nevertheless, no studies have yet been conducted which have examined the dynamics of CSBD healing after LIPUS application nor have any studies compared this defect after AB graft implementation.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Low-Intensity Pulsed Ultrasound on Bone Regeneration and the Expression of Osterix and Cyclooxygenase-2 during Critical-Size Bone Defect Repair

Volarić,

Žauhar,

Chen

et al. 2024

IJMS

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Low-intensity pulsed ultrasound (LIPUS) is a form of ultrasound that utilizes low-intensity pulsed waves. Its effect on bones that heal by intramembranous ossification has not been sufficiently investigated. In this study, we examined LIPUS and the autologous bone, to determine their effect on the healing of the critical-size bone defect (CSBD) of the rat calvaria. The bone samples underwent histological, histomorphometric and immunohistochemical analyses. Both LIPUS and autologous bone promoted osteogenesis, leading to almost complete closure of the bone defect. On day 30, the bone volume was the highest in the autologous bone group (20.35%), followed by the LIPUS group (19.12%), and the lowest value was in the control group (5.11%). The autologous bone group exhibited the highest intensities of COX-2 (167.7 ± 1.1) and Osx (177.1 ± 0.9) expression on day 30. In the LIPUS group, the highest intensity of COX-2 expression was found on day 7 (169.7 ±1.6) and day 15 (92.7 ± 2.2), while the highest Osx expression was on day 7 (131.9 ± 0.9). In conclusion, this study suggests that LIPUS could represent a viable alternative to autologous bone grafts in repairing bone defects that are ossified by intramembranous ossification.

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Enhanced bone formation of calvarial bone defects by low-intensity pulsed ultrasound and recombinant human bone morphogenetic protein-9: a preliminary experimental study in rats

Cited by 4 publications

References 49 publications

Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells

Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells

The role of Bone Morphogenetic Protein-2 in craniofacial osteogenesis: A literature review

The Effect of Low-Intensity Pulsed Ultrasound on Bone Regeneration and the Expression of Osterix and Cyclooxygenase-2 during Critical-Size Bone Defect Repair

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