Enhanced bone healing of rat tooth sockets after administration of epidermal growth factor (EGF) carried by liposome

Marquez, Luciana; Abreu, Fernando Antônio Mauad de; Ferreira, Cynthia Lopes; Alves, Guilherme Dias; Miziara, Melissa Nunes; Alves, José Bento

doi:10.1016/j.injury.2012.10.014

Cited by 34 publications

(37 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The bone tissue repair process is a complex cascade of biological events controlled by numerous cytokines and growth factors that provide local signals to mediate migration of osteoprogenitor cells and subsequent differentiation, cell proliferation, revascularization and production of extracellular matrix [34]. Several growth factors, such as Bone Morphogenic Proteins (BMPs) [35]–[37] platelet-derived growth factor (PDGF) [35]–[37], transforming growth factor-beta (TGF-ß) [35], [36], insulin like growth factor (IGF) [36], [38], vascular endothelial growth factor (VEGF) [35]–[38], endothelial cell growth factor (ECGF) [36], fibroblast growth factor (FGF) [34], [36], [37] and epidermal growth factor (EGF) [34] are expressed during development and bone repair, and their local availability in the wound might collaborate for faster and successful bone healing [34].…”

Section: Discussionmentioning

confidence: 99%

Paracrine Effect of Mesenchymal Stem Cells Derived from Human Adipose Tissue in Bone Regeneration

2014

View full text Add to dashboard Cite

Mesenchymal stem cell (MSC) transplantation has proved to be a promising strategy in cell therapy and regenerative medicine. Although their mechanism of action is not completely clear, it has been suggested that their therapeutic activity may be mediated by a paracrine effect. The main goal of this study was to evaluate by radiographic, morphometric and histological analysis the ability of mesenchymal stem cells derived from human adipose tissue (Ad-MSC) and their conditioned medium (CM), to repair surgical bone lesions using an in vivo model (rabbit mandibles). The results demonstrated that both, Ad-MSC and CM, induce bone regeneration in surgically created lesions in rabbit's jaws, suggesting that Ad-MSC improve the process of bone regeneration mainly by releasing paracrine factors. The evidence of the paracrine effect of MSC on bone regeneration has a major impact on regenerative medicine, and the use of their CM can address some issues and difficulties related to cell transplants. In particular, CM can be easily stored and transported, and is easier to handle by medical personnel during clinical procedures.

show abstract

Section: Discussionmentioning

confidence: 99%

Paracrine Effect of Mesenchymal Stem Cells Derived from Human Adipose Tissue in Bone Regeneration

2014

View full text Add to dashboard Cite

show abstract

“…Such bivalent systems are then able to support cells and also provide growth and differentiation factors . Studies using several types of NPs such as polymeric, liposomes, silica, and calcium phosphate ceramic NPs indicated that the use of nanoparticulate systems as carriers allow a sustained delivery of their cargo. Table summarizes the outcomes of NPs used as drug and gene carriers.…”

Section: Applications For Bone Tementioning

confidence: 99%

“…In the latter case, the liposomes allowed a controlled release of EGF that stimulated MSCs migration and osteoblast differentiation. While free exogenous EGF in PBS was cleared faster and did not lead to full wound regeneration, EGF entrapped in liposomes allowed faster recovery, with approximately 50% more bone trabeculae present after 21 days …”

Section: Applications For Bone Tementioning

confidence: 99%

“…While free exogenous EGF in PBS was cleared faster and did not lead to full wound regeneration, EGF entrapped in liposomes allowed faster recovery, with approximately 50% more bone trabeculae present after 21 days. 145 Extracellular matrix molecules can also be used for bone regeneration improvement. Osteopontin, a key player in the bone remodeling process, was used to functionalize HAp NPs that were further mixed with poly-L-D-lactic acid (PDLLA) to create an implant coating.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Nanoparticles for bone tissue engineering

Vieira¹,

Vial²,

Reis³

et al. 2017

Biotechnology Progress

170

137

View full text Add to dashboard Cite

Tissue engineering (TE) envisions the creation of functional substitutes for damaged tissues through integrated solutions, where medical, biological, and engineering principles are combined. Bone regeneration is one of the areas in which designing a model that mimics all tissue properties is still a challenge. The hierarchical structure and high vascularization of bone hampers a TE approach, especially in large bone defects. Nanotechnology can open up a new era for TE, allowing the creation of nanostructures that are comparable in size to those appearing in natural bone. Therefore, nanoengineered systems are now able to more closely mimic the structures observed in naturally occurring systems, and it is also possible to combine several approaches - such as drug delivery and cell labeling - within a single system. This review aims to cover the most recent developments on the use of different nanoparticles for bone TE, with emphasis on their application for scaffolds improvement; drug and gene delivery carriers, and labeling techniques. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:590-611, 2017.

show abstract

“…Many studies have suggested that the local concentration of EGF needs to be sufficiently high and prolonged for effective wound healing [126,132,133]. Therefore, different drug delivery systems that are able to protect and stabilize the protein have been investigated, including hydrogels, sponges, polymeric pellets, nanofibers, microspheres, a biomimetic delivery system that incorporated EGF with biocompatible components such as hyaluronic acid, collagen, vitronectin or liposome, and more recently, a poloxamer gel formulation of recombinant low-molecular-weight protamine conjugated EGF (rLMWP-EGF) by conjugating a highly positively charged rLMWP to the N-terminal of EGF [134–140]. The latter has shown better healing effects on burn injuries [139].…”

Section: Egf Clinical Relevancementioning

confidence: 99%

Epidermal growth factor, from gene organization to bedside

Zeng

Harris

2014

Seminars in Cell & Developmental Biology

168

126

View full text Add to dashboard Cite

In 1962, epidermal growth factor (EGF) was discovered by Dr. Stanley Cohen while studying nerve growth factor (NGF). It was soon recognized that EGF is the prototypical member of a family of peptide growth factors that activate the EGF receptors, and that the EGF/EGF receptor signaling pathway plays important roles in proliferation, differentiation and migration of a variety of cell types, especially in epithelial cells. After the basic characterization of EGF function in the first decade or so after its discovery, the studies related to EGF and its signaling pathway have extended to a broad range of investigations concerning its biological and pathophysiological roles in development and in human diseases. In this review, we briefly describe the gene organization and tissue distribution of EGF, with emphasis on its biological and pathological roles in human diseases.

show abstract

Enhanced bone healing of rat tooth sockets after administration of epidermal growth factor (EGF) carried by liposome

Cited by 34 publications

References 26 publications

Paracrine Effect of Mesenchymal Stem Cells Derived from Human Adipose Tissue in Bone Regeneration

Paracrine Effect of Mesenchymal Stem Cells Derived from Human Adipose Tissue in Bone Regeneration

Nanoparticles for bone tissue engineering

Epidermal growth factor, from gene organization to bedside

Contact Info

Product

Resources

About