Enhanced bone morphogenic property of parylene-C

Abstract

The ability to induce osteointegration was introduced to a parylene-C surface via the simple and intuitive process of protein adsorption mediated by hydrophobic interactions. In this way, bone morphogenetic protein (BMP)-2, fibronectin, and platelet-rich plasma (PRP) could be immobilized on parylene-C surfaces. This approach alleviates concerns related to the use of potentially harmful substances in parylene-C modification processes. The adsorbed protein molecules were quantitatively characterized with respect to adsorption efficacy and binding affinity, and the important biological activities of the proteins were also examined using both early and late markers of osteogenetic activity, including alkaline phosphatase expression, calcium mineralization and marker gene expression. Additionally, the adsorbed PRP exhibited potential as a substitute for expensive recombinant growth factors by effectively inducing comparable osteogenetic activity. In addition to the excellent biocompatibility of parylene-C and its ability to coat a wide variety of substrate materials, the modification of parylene-C via protein adsorption provides unlimited possibilities for installing specific biological functions, expanding the potential applications of this material to include various biointerface platforms.

Bio-Interface on Freestanding Nanosheet of Microelectromechanical System Optical Interferometric Immunosensor for Label-Free Attomolar Prostate Cancer Marker Detection

“…The error bars indicate the standard deviations. In the pristine parylene-C layer, the surface zeta potential was −18.3 mV, which is consistent with the values of other studies [ 42 , 43 ]. When diX-AM was deposited on parylene-C, the zeta potential reversed and increased to +25.1 mV.…”

Fabrication of Asymmetrical and Gradient Hierarchy Structures of Poly-p-xylylenes on Multiscale Regimes Based on a Vapor-Phase Sublimation and Deposition Process

“…By using a suspension ice template for the sublimation and deposition processes to produce porous poly- p -xylylene, hierarchical pore sizes were generated from voids left by (i) nonsublimating oil with the size controlled by dispersion methods, such as sonication or emulsion, ,, and (ii) sublimated water vapor with the size controlled by the sublimation conditions . The demonstration of shaping the suspension into a spherical drop was enabled by spraying the suspension onto a hydrophobic surface, thus resulting in a solidified ice particle (template); subsequently, the ice particles of templates in the fabrication process were transformed to produce porous polychloro- p -xylylene (a characteristic derivative of poly- p -xylylene) , particles with hierarchical interior structures (Figure A). Shaping the solid templates can also be intuitively performed by molding, sculpting, or engineering interactions between a liquid and a substrate surface to obtain solidified particles or other geometries, and the size is theoretically limited by selected methods.…”

Vapor construction and modification of stem cell-laden multicomponent scaffolds for regenerative therapeutics

“…The fabricated scaffold group modified with FGF-2 and A2-P (i.e., hASC-BM scaffolds) exhibited a stronger fluorescent signal for osteocalcin expression than the control group (i.e., hASC scaffolds), which does not accommodate FGF-2 and A2-P. In comparison to previous reports, characterizations by examining the alkaline phosphatase (ALP) activities were also shown with upregulated enhancement based on using similar poly- p -xylylene platform with BMP-2 or PRP (platelet-rich plasma) modifications [ [51] , [52] , [53] , [54] ], and are unambiguously comparable to the results in the current work. Adipogenic activity was examined by Oil Red O staining after 10 days of culture in adipogenic medium.…”