Enhanced bone regeneration via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts

Egusa, Hiroshi; Kaneda, Yoshitoshi; Akashi, Yoshihiro; Hamada, Y.; Matsumoto, Takuya; Saeki, Makio; Thakor, Devang K.; Tabata, Yasuhiko; Matsuura, Nariaki; Yatani, Hirofumi

doi:10.1016/j.biomaterials.2009.05.032

Cited by 63 publications

(53 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There have been investigative reports of ectopic bone growth with rhBMP-2 and, consequently, its use in growing patients is being studied carefully [41]. In order to overcome some of these difficulties, a variety of pre-clinical studies are carried out; such as testing new optimized carrier systems to decrease the dosage of growth factors, producing the growth factors by using E-coli system [42,43], or finding the new bioactive small peptide which have osseoinduction ability [44] to reduce the production costs and developing a genetically engineered mutant growth factors to improve the binding property to the extracellular matrix in order to prevent rapid diffusion.…”

Section: Discussionmentioning

confidence: 99%

The effect of growth factors for bone augmentation to enable dental implant placement: A systematic review

Shimono

Oshima

Arakawa

et al. 2010

Japanese Dental Science Review

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

The effect of growth factors for bone augmentation to enable dental implant placement: A systematic review

Shimono

Oshima

Arakawa

et al. 2010

Japanese Dental Science Review

View full text Add to dashboard Cite

“…These limitations can be overcome by using small peptides for medical treatment. 11 Therefore, the results of this study demonstrated that in situ conjugation of the SVVYGLR peptide to injectable GPT hydrogels influenced HUVEC activity and enhanced angiogenic activity in the matrix. In addition, the method developed in this study provides a simple tool for constructing bioactive injectable materials containing angiogenic factors for enhanced neo-vascularization, which is an important achievement in the field of tissue regenerative medicine.…”

Section: Scheme 2 Schematic Representation Of the In Situ Sv−y Conjumentioning

confidence: 93%

In Situ SVVYGLR Peptide Conjugation into Injectable Gelatin-Poly(ethylene glycol)-Tyramine Hydrogel via Enzyme-Mediated Reaction for Enhancement of Endothelial Cell Activity and Neo-Vascularization

et al. 2012

View full text Add to dashboard Cite

Tissue engineering therapies require biocompatible and bioactive biomaterials that are capable of encouraging an angiogenic response for effective tissue regeneration. In this study, a SVVYGLR peptide, which functions as a potent angiogenic factor, was conjugated into injectable gelatin-poly(ethylene glycol)-tyramine (GPT) hydrogels in situ to enhance endothelial cell activities and neo-vascularization. SVVYGLRGGY (SV-Y) conjugated GPT (SV-GPT) hydrogels were formed in situ via enzyme-mediated reaction using horseradish peroxidase (HRP) and hydrogen peroxide (H(2)O(2)). The physico-chemical properties were characterized and could be controlled depending on the feed peptide and H(2)O(2) concentration. The concentration of conjugated peptide ranged from 0.37 to 0.81 μmol/mL, and the elastic moduli (G') of the hydrogels were 600-4900 Pa. In vitro cell studies using human umbilical vein endothelial cells (HUVECs) and in vivo subcutaneous injection studies were performed to confirm the effect of the SVVYGLR peptide on HUVEC activity and neo-vascularization. Obtained results demonstrated that the in situ conjugation of SVVYGLR sequences into phenol residues of GPT hydrogels enhanced the activity of HUVECs in vitro and stimulated the formation of new blood vessels in the hydrogel matrices in vivo. From the results, we suggest that in situ conjugation of SV-Y to GPT hydrogels via the enzymatic reaction may be an efficient tool to prepare injectable bioactive hydrogels that can enhance endothelial cell activities and promoting angiogenesis for tissue regeneration.

show abstract

“…The culture medium was renewed every other day. The number of cells was evaluated using the WST-1 cell counting assay (Dojindo Laboratories, Kumamoto, Japan) as described previously [50].…”

Section: Methodsmentioning

confidence: 99%

Gingival Fibroblasts as a Promising Source of Induced Pluripotent Stem Cells

et al. 2010

Self Cite

View full text Add to dashboard Cite

BackgroundInduced pluripotent stem (iPS) cells efficiently generated from accessible tissues have the potential for clinical applications. Oral gingiva, which is often resected during general dental treatments and treated as biomedical waste, is an easily obtainable tissue, and cells can be isolated from patients with minimal discomfort.Methodology/Principal FindingsWe herein demonstrate iPS cell generation from adult wild-type mouse gingival fibroblasts (GFs) via introduction of four factors (Oct3/4, Sox2, Klf4 and c-Myc; GF-iPS-4F cells) or three factors (the same as GF-iPS-4F cells, but without the c-Myc oncogene; GF-iPS-3F cells) without drug selection. iPS cells were also generated from primary human gingival fibroblasts via four-factor transduction. These cells exhibited the morphology and growth properties of embryonic stem (ES) cells and expressed ES cell marker genes, with a decreased CpG methylation ratio in promoter regions of Nanog and Oct3/4. Additionally, teratoma formation assays showed ES cell-like derivation of cells and tissues representative of all three germ layers. In comparison to mouse GF-iPS-4F cells, GF-iPS-3F cells showed consistently more ES cell-like characteristics in terms of DNA methylation status and gene expression, although the reprogramming process was substantially delayed and the overall efficiency was also reduced. When transplanted into blastocysts, GF-iPS-3F cells gave rise to chimeras and contributed to the development of the germline. Notably, the four-factor reprogramming efficiency of mouse GFs was more than 7-fold higher than that of fibroblasts from tail-tips, possibly because of their high proliferative capacity.Conclusions/SignificanceThese results suggest that GFs from the easily obtainable gingival tissues can be readily reprogrammed into iPS cells, thus making them a promising cell source for investigating the basis of cellular reprogramming and pluripotency for future clinical applications. In addition, high-quality iPS cells were generated from mouse GFs without Myc transduction or a specific system for reprogrammed cell selection.

show abstract

Enhanced bone regeneration via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts

Cited by 63 publications

References 39 publications

The effect of growth factors for bone augmentation to enable dental implant placement: A systematic review

The effect of growth factors for bone augmentation to enable dental implant placement: A systematic review

In Situ SVVYGLR Peptide Conjugation into Injectable Gelatin-Poly(ethylene glycol)-Tyramine Hydrogel via Enzyme-Mediated Reaction for Enhancement of Endothelial Cell Activity and Neo-Vascularization

Gingival Fibroblasts as a Promising Source of Induced Pluripotent Stem Cells

Contact Info

Product

Resources

About