Gingival Fibroblasts as a Promising Source of Induced Pluripotent Stem Cells

Egusa, Hiroshi; Okita, Keisuke; Kayashima, Hiroki; Yu, Guoqiang; Fukuyasu, Sho; Saeki, Makio; Matsumoto, Takuya; Yamanaka, Shinya; Yatani, Hirofumi

doi:10.1371/journal.pone.0012743

Cited by 144 publications

(122 citation statements)

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“…It has also been shown that recombinant laminin E8 fragments (LM-E8s), which are truncated peptides composed of the C-terminal regions of the α, β, and γ chains of laminin, enable robust propagation of human ESCs and iPSCs in an undifferentiated state in cultures with defined and xeno-free media [68]. We have confirmed that human gingiva-derived iPSCs [30] can be maintained in an undifferentiated state on LM-E8-coated plates after dissociation and passaging ( Fig. 12.2).…”

Section: Recombinant Ecm Productssupporting

confidence: 57%

“…In contrast to the case with mouse ESCs, LIF does not satisfactorily support the self-renewal of human ESCs [4]. Nonetheless, SNL76/7 cells basically inherit the nature of STO cells; therefore, SNL 76/7 cells can be used as feeder cells for both mouse [6,30,31] and human [1,28,30] ESCs/iPSCs. Bradley also established a puromycin-resistant derivative of SNL76/7 cells (SNLP76/7-4 cell line: Fig.…”

Section: Mouse-derived Feeder Cellsmentioning

confidence: 90%

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Feeder Cell Sources and Feeder-Free Methods for Human iPS Cell Culture

Kamano

Wang

et al. 2015

Interface Oral Health Science 2014

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Induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine and disease modeling. The original methods to grow human iPSCs utilized methods developed for human embryonic cells (ESCs), in which mitotically inactivated mouse-derived fibroblasts are mainly used as a "feeder" cell layer to maintain the undifferentiated status of pluripotent stem cells. However, these methods still require further consideration to facilitate cell expansion and to maintain the undifferentiated state of human iPSCs and/or ESCs for a longer period of time. In addition, the use of animal-derived feeders should be avoided for eventual clinical application of iPSC therapies. Therefore, human-derived feeder culture systems or feeder-free culture systems are currently being developed to prevent exposure to animal pathogens. In this review, existing mouse and human feeder culture systems for human ESCs and iPSCs are first introduced, and then previously reported feeder-free culture methods using extracellular matrixassociated products or synthetic biomaterials are outlined to discuss an appropriate culture system for clinical application of iPSCs.

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Section: Recombinant Ecm Productssupporting

confidence: 57%

Section: Mouse-derived Feeder Cellsmentioning

confidence: 90%

Feeder Cell Sources and Feeder-Free Methods for Human iPS Cell Culture

Kamano

Wang

et al. 2015

Interface Oral Health Science 2014

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“…Therefore, iPS cells offer the potential to derive patient-and lineage-specific cells for clinical applications and have emerged as a potential cell source for regenerative medicine and tissue engineering. Previous studies suggested that cell proliferation ability (42,59) or endogenous expression of reprogramming factors (10,55) in parent cells is positively related to the reprogramming efficiency. Human dental pulp cells as parent cells for iPS cells exhibit higher reprogramming efficiency than do human dermal fibroblasts (42,55) because they show rapid cell proliferation (42) and high expression of endogenous reprogramming factors, c-MYC and KLF4 (55), compared to human dermal fibroblasts.…”

Section: Methodsmentioning

confidence: 99%

<b>Induction of neural crest cells from human dental pulp-derived induced pluripotent stem </b><b>cells </b>

et al. 2017

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We previously generated induced pluripotent stem (iPS) cells from human dental pulp cells of deciduous teeth. Neural crest cells (NCCs) play a vital role in the development of the oral and maxillofacial region. Therefore, NCCs represent a cell source for bone, cartilage, and tooth-related tissue engineering. In this study, we examined whether iPS cells are capable of differentiating into NCCs through modification of the human embryonic stem cell protocol. First, iPS cells were dissociated into single cells and then reaggregated in low-cell-adhesion plates with neural induction medium for 8 days in suspension culture to form neurospheres. The neurospheres were transferred to fibronectin-coated dishes and formed rosette structures. The migrated cells from the rosettes abundantly expressed NCC markers, as evidenced by real-time polymerase chain reaction, immunofluorescence, and flow cytometric analysis. Furthermore, the migrated cells exhibited the ability to differentiate into neural crest lineage cells in vitro. They also exhibited tissue-forming potential in vivo, differentiating into bone and cartilage. Collectively, the migrated cells had similar characteristics to those of NCCs. These results suggest that human dental pulp cell-derived iPS cells are capable of differentiating into NCCs. Therefore, iPS cell-derived NCCs represent cell sources for bone and cartilage tissue engineering.

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“…19,20) Briefly, OM cells were seeded into the wells (1 × 10 5 cells /well) of 6-well plates, and were cultured in DMEM with 10% FBS and 1% penicillin-streptomycin at 37°C with 5% CO 2 for 24 h. The (on average, less than 3 weeks). iPS colonies were selected based on mouse ES cell-like colony morphology, and medium was changed every day.…”

Section: Reprogramming Of Mouse Oral Mucosa (Om) Cellsmentioning

confidence: 99%

Regulation of Nodal-Lefty Expression by TGF-^|^beta; Induced Pluripotent Stem (iPS) Cells Derived from Mouse Oral Mucosal Tissue

Okada

Ochiai

Saito

et al. 2012

J. Hard Tissue Biology.

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Nodal-Lefty signaling is essential for embryonic stem cell pluripotency and growth control of ES cells, which play an important role in tumorigenic phenotype. We recently reported that TGF-increases Lefty expression in pancreatic cancer cells. However, a previous study has shown that TGF-is unable to induce Nodal and Lefty expression in ES or induced pluripotent stem (iPS) cells. We established an iPS cell line from mouse oral tissue via retroviral gene transfer of OCT3/4, Sox2, c-Myc and KLF4. The obtained iPS cells expressed undifferentiated markers, such as OCT4, Nanog and SSEA1. We observed induction of the Lefty gene in these iPS cells, as well as in mouse ES cells in ES medium either with or without TGF-treatment. This is the first report showing Lefty expression in iPS cells. We then investigated the signaling relationship between TGF-and mitogen-activated protein kinase (MAPK) by using specific kinase inhibitors, and found that p38 has some effect on Lefty expression. Taken together, these results suggest that iPS cells from oral tissue possess a different mechanism for Lefty expression than mouse ES cells. TGF-with p38 is able to regulate Lefty expression in iPS cells.

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Gingival Fibroblasts as a Promising Source of Induced Pluripotent Stem Cells

Cited by 144 publications

References 50 publications

Feeder Cell Sources and Feeder-Free Methods for Human iPS Cell Culture

Feeder Cell Sources and Feeder-Free Methods for Human iPS Cell Culture

<b>Induction of neural crest cells from human dental pulp-derived induced pluripotent stem </b><b>cells </b>

Regulation of Nodal-Lefty Expression by TGF-^|^beta; Induced Pluripotent Stem (iPS) Cells Derived from Mouse Oral Mucosal Tissue

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