Enhanced chondrogenic responses of articular chondrocytes onto porous silk fibroin scaffolds treated with microwave-induced argon plasma

Baek, Hyun Sook; Park, Young Hwan; Ki, Chang Seok; Park, Jong Chul; Rah, Dong Kyun

doi:10.1016/j.surfcoat.2008.06.154

Cited by 87 publications

(60 citation statements)

References 21 publications

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“…[16][17][18] SF nanofibers are viable substrates to culture chondrocytes, osteoblasts, and mesenchymal stem cells to enhance cell attachment and proliferation. 19 Though nanofibrous scaffolds are favored by many investigations due to their high interconnectivity, collagenous environment, and resemblance to natural tissues, they are weak under mechanical loadings. Thus, in order to use fibrous scaffolds when subject to tensional/compressional stresses in bone tissue engineering application, enhancement in scaffold mechanical properties is highly desired.…”

Section: Introductionmentioning

confidence: 99%

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Lai

Shalumon

Chen

2015

IJN

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Incorporation of nanohydroxyapatite (nHAP) within a chitosan (CS)/silk fibroin (SF) nanofibrous membrane scaffold (NMS) may provide a favorable microenvironment that more closely mimics the natural bone tissue physiology and facilitates enhanced osteogensis of the implanted cell population. In this study, we prepared pristine CS/SF NMS, composite CS/SF/nHAP NMS containing intrafibrillar nHAP by in situ blending of 10% or 30% nHAP before the electrospinning step, and composite CS/SF/nHAP NMS containing extrafibrillar nHAP by depositing 30% nHAP through alternative soaking surface mineralization. We investigated the effect of the incorporation of HAP nanoparticles on the physicochemical properties of pristine and composite NMS. We confirmed the presence of ~30 nm nHAP in the composite nanofibrous membranes by thermogravimetry analysis (TGA), X-ray diffraction (XRD), and scanning electron microscopy (SEM), either embedded in or exposed on the nanofiber. Nonetheless, the alternative soaking surface mineralization method drastically influenced the mechanical properties of the NMS with 88% and 94% drop in Young’s modulus and ultimate maximum stress. Using in vitro cell culture experiments, we investigated the effects of nHAP content and location on proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs). The proliferation of hMSCs showed no significant difference among pristine and composite NMS. However, the extent of osteogenic differentiation of hMSCs was found to be positively correlated with the content of nHAP in the NMS, while its location within the nanofiber played a less significant role. In vivo experiments were carried out with hMSCs seeded in CS/SF/30%nHAP NMS prepared by in situ blending and subcutaneous implantation in nude mice. Micro-computed tomography images as well as histological and immunohistochemical analysis of the retrieved hMSCs/NMS construct 1 and 2 months postimplantation indicated that NMS had the potential for bone regeneration and can be suggested as a promising scaffold for bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Lai

Shalumon

Chen

2015

IJN

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“…This method provides a wide range of surface functionalities, which can improve biocompatibility either directly or indirectly through biomolecule surface immobilization. For instance, surface functionalization with hydrophilic chemical groups (e.g., -COOH and -NH 2 ) by reactive gas plasma treatment or surface chemical modification by film deposition [2][3][4][5][6][7][8][9][10][11] and coating of polymer surfaces by various extracellular matrix proteins (e.g., collagen, gelatin, and laminin) [12][13][14][15][16][17][18] and other bioactive molecules by plasma treatment 19,20 have been shown to improve the biocompatibility of polymer materials.…”

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confidence: 99%

“…2,3,8 Furthermore, plasma-synthesized polymer coatings rich in -NH 2 and -COOH surface groups have been reported to promote cell growth on scaffold surfaces. [9][10][11] However, relatively less is known about the effect of inert gas plasma treatment of polymers on cell growth, 5,7 and information about the effect of inert or reactive gas plasmas on cell infiltration in three-dimensional structures is sparse. The intense conditions of the inert gas plasmas used in previous studies to produce detectable chemistry modification induced structural damage and/or roughening of the polymer fibers due to thermal heating and excessive plasma etching, respectively.…”

mentioning

confidence: 99%

Plasma Surface Chemical Treatment of Electrospun Poly(l-Lactide) Microfibrous Scaffolds for Enhanced Cell Adhesion, Growth, and Infiltration

Cheng

Lee

Komvopoulos

et al. 2013

Tissue Engineering Part A

100

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Poly(l-lactide) (PLLA) microfibrous scaffolds produced by electrospinning were treated with mild Ar or Ar-NH 3 /H 2 plasmas to enhance cell attachment, growth, and infiltration. Goniometry, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) measurements were used to evaluate the modification of the scaffold surface chemistry by plasma treatment. AFM and XPS measurements showed that both plasma treatments increased the hydrophilicity without affecting the integrity of the fibrous structure and the fiber roughness, whereas Ar-NH 3 /H 2 plasma treatment also resulted in surface functionalization with amine groups. Culture studies of bovine aorta endothelial cells and bovine smooth muscle cells on the plasma-treated PLLA scaffolds revealed that both Ar and Ar-NH 3 /H 2 plasma treatments promoted cell spreading during the initial stage of cell attachment and, more importantly, increased the cell growth rate, especially for Ar plasma treatment. In vitro cell infiltration studies showed that both plasma treatments effectively enhanced cell migration into the microfibrous scaffolds. In vivo experiments involving the subcutaneous implantation of plasma-treated PLLA scaffolds under the skin of Sprague-Dawley rats also showed increased cell infiltration. The results of this study indicate that surface treatment of PLLA microfibrous scaffolds with mild Ar or Ar-NH 3 /H 2 plasmas may have important implications in tissue engineering. Further modifications with bioactive factors should improve the functions of the scaffolds for specific applications. Introduction Microfibrous structures synthesized by electrospinning are of particular interest in bioengineering due to their high porosity and biodegradability that make them ideal candidates for polymer scaffolds. However, because polymer surfaces (solid or fibrous), such as poly(llactide) (PLLA), are hydrophobic, cell attachment and growth on polymer scaffolds is limited. Therefore, various surface treatments have been used to modify the chemical behavior of PLLA surfaces in order to improve biocompatibility.1 Plasma-assisted surface modification is a common method of tuning biochemical surface properties to specific application needs. This method provides a wide range of surface functionalities, which can improve biocompatibility either directly or indirectly through biomolecule surface immobilization. For instance, surface functionalization with hydrophilic chemical groups (e.g., -COOH and -NH 2 ) by reactive gas plasma treatment or surface chemical modification by film deposition 2-11 and coating of polymer surfaces by various extracellular matrix proteins (e.g., collagen, gelatin, and laminin) [12][13][14][15][16][17][18] and other bioactive molecules by plasma treatment 19,20 have been shown to improve the biocompatibility of polymer materials.In addition to studies devoted to the increase of the surface hydrophilicity of biopolymers for promoting bioactive molecular and protein attachment, the direct effect of plasma surface treatment on biocompati...

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“…In general, biopolymers are frequently used to fabricate scaffolds via other scaffolding techniques (e.g., rapid prototyping), but due to their high water content, it is less obvious to apply a non-thermal plasma, whereas electrospun materials are normally used in a dehydrated state. Baek et al used an MW induced Ar plasma jet to modify electrospun/salt leached silk fibroin scaffolds (400 µm) intended for cartilage repair [91,92]. Neonatal human knee articular chondrocytes were seeded onto scaffolds, resulting in a 50 % increase of initial cell attachment and a 100 % increase of proliferation compared to untreated scaffolds.…”

Section: Electrospinningmentioning

confidence: 99%

Plasma Science and Technology - Progress in Physical States and Chemical Reactions

Mieno¹

2016

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Non-thermal plasma technology is one of those techniques that suffer relatively little from diffusion limits, slow kinetics, and complex geometries compared to more traditional liquid-based chemical surface modification techniques. Combined with a lack of solvents, preservation of the bulk properties, and fast treatment times; it is a well-liked technique for the treatment of materials for biomedical applications. In this book chapter, a review will be given on what the scientific community determined to be essential to obtain appropriate scaffolds for tissue engineering and how plasma scientists have used non-thermal plasma technology to accomplish this. A distinction will be made depending on the scaffold fabrication technique, as each technique has its own set of specific problems that need to be tackled. Fabrication techniques will include traditional fabrication methods, rapid prototyping, and electrospinning. As for the different plasma techniques, both plasma activation and grafting/polymerization will be included in the review and linked to the in-vitro/in-vivo response to these treatments. The literature review itself is preceded by a more general overview on cell communication, giving useful insights on how surface modification strategies should be developed.

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Enhanced chondrogenic responses of articular chondrocytes onto porous silk fibroin scaffolds treated with microwave-induced argon plasma

Cited by 87 publications

References 21 publications

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

Plasma Surface Chemical Treatment of Electrospun Poly(l-Lactide) Microfibrous Scaffolds for Enhanced Cell Adhesion, Growth, and Infiltration

Plasma Science and Technology - Progress in Physical States and Chemical Reactions

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