Enhanced co-production of pectinase, cellulase and xylanase enzymes from Bacillus subtilis ABDR01 upon ultrasonic irradiation

Yadav, Ashish; Ali, Anees Ahmed Mahaboob; Ingawale, Mrunal; Raychaudhuri, Sarmistha Sen; Gantayet, L.M.; Pandit, Aniruddha B.

doi:10.1016/j.procbio.2020.01.011

Cited by 30 publications

(21 citation statements)

References 28 publications

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“…The production of Daunorubicin by Streptomyces peucetius MTCC 4332 was also improved when the US power output was raised from 40 W to 160 W 40 . Conversely, the increase on power output beyond 90 W showed detrimental effects on co‐production of pectinase, xylanase, and cellulase by Bacillus subtilis ABDR01 41 . With higher ultrasound power, a larger number of microbubbles are formed.…”

Section: Resultsmentioning

confidence: 99%

Ultrasound‐assisted fermentation for production of β‐1,3‐glucanase and chitinase by Beauveria bassiana

Schmaltz

Aita

Alves

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUND: Ultrasound (US) treatment was applied to increase the production of ⊎-1,3-glucanase and chitinase by Beauveria bassiana (IBCB 66) cultivated in submerged fermentation. Application of US at different growth stages, US power outputs, time of ultrasonication, and duty cycles were the parameters evaluated. RESULTS: The best strategy comprised ultrasonication during 5 min at 24 h of fermentation with a power output of 195 W (fixed frequency of 24 kHz) and a duty cycle of 0.5 s.s −1 (0.5 s ON, 0.5 s OFF). The US treatment resulted in a production increase of 46% for ⊎-1,3-glucanase and 42% for chitinase compared to the non-sonicated control. Fragmentations of the mycelia in the sonicated samples were observed in Scanning Electron Microscopy (SEM) micrographs after the US application, supporting the idea of a better release of enzymes to outside the cell. CONCLUSION: Ultrasound-assisted fermentation was a promising tool to increase the production of ⊎-1,3-glucanase and chitinase by Beauveria bassiana (IBCB 66). The combination of US parameters showed to be decisive in obtaining higher enzyme production when compared to the control non-sonicated experiment. Therefore, this work brings a significant contribution to the field of stimulation of bioprocesses with ultrasound since it shows a strategy to obtain higher enzyme production when compared to traditional fermentation.

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Section: Resultsmentioning

confidence: 99%

Ultrasound‐assisted fermentation for production of β‐1,3‐glucanase and chitinase by Beauveria bassiana

Schmaltz

Aita

Alves

et al. 2020

J of Chemical Tech & Biotech

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“…Two well-characterized bacterial strains of genus Bacillus namely Bacillus subtilis ABDR01 [ 25 ] from aerosol of Department of Microbiology, Tripura University, India (23°45′ N, 91°15′E) and Bacillus thuringiensis MCC2138 [ 3 ] isolated from the coastal marine water of Mandarmani, Midnapur, West Bengal, India (21°37.012′N, 87°29.881′E) [ 28 ] were used for consortium development in 1:1 ratio. Consortium performance was analyzed for their extracellular enzyme-producing ability (pectinase, cellulase, xylanase, and amylase) under suspended and immobilized conditions [ 25 ]. The consortium performance was compared with single isolates for each enzyme to understand the interaction between the consortium's bacteria.…”

Section: Methodsmentioning

confidence: 99%

“…Research interests in Ramie fibers are growing steadily owing to their potential about sustainability and eco-friendly applications [ 24 ]. Ongoing research strives to improve the texture and quality of the finished product through enzymatic degumming to be commercialized [ 3 , 25 , 26 ]. Multiple bacterial strains were tested to assess Ramie gum removal/gum utilization efficiency [ 3 , 24 , 25 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…Ongoing research strives to improve the texture and quality of the finished product through enzymatic degumming to be commercialized [ 3 , 25 , 26 ]. Multiple bacterial strains were tested to assess Ramie gum removal/gum utilization efficiency [ 3 , 24 , 25 , 27 ]. Previous studies by the group reported Bacillus thuringiensis MCC2138 to efficiently degum Ramie fiber within 72 h of incubation [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…The fully chemically treated fiber had fibrils released, which gave it the matt finish and coarse texture. Another study revealed the extracellular enzyme-producing ability of Bacillus subtilis ABDR01 [ 25 ]. Elongation percentage of the fiber treated with ABDR01 was 1.44 % ± 0.27.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of bio-chemical degumming of Ramie fiber for improved strength & luster

Chaudhuri

Gogoi

Biswas

et al. 2020

Biotechnology Reports

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Multipurpose cellulases of Promicromonospora sp. VP111, with broad substrate specificity and tolerance properties

2023

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Cellulolytic actinobacterium, Promicromonospora sp. VP111 concomitantly produced cellulases (CELs), xylanase and pectinase when grown on commercial cellulose and untreated agricultural lignocellulosic residues (wheat straw and sugarcane bagasse). Secreted CELs hydrolyzed (enhanced with Co 2+ ion) multiple cellulosic substrates, including sodium carboxymethyl cellulose (Na-CMC), Whatman filter paper no. 1, microcrystalline cellulose (avicel), p-nitrophenyl-β-D-glucopyranoside (pNPG), laminarin, and cellulose powder. The CELs showed stabilities in the presence of various chemicals, including glucose (0.2 M), detergents (1%, w/v or v/v), denaturants (1%, w/v or v/v), and sodium chloride (NaCl, 30%, w/v). The CELs were fractionated using ammonium sulfate precipitation and dialysis. Activities (%) of fractionated CELs were retained at 60°C for endoglucanase/carboxymethyl cellulase (CMCase) (88.38), filter paper cellulase (FPase) (77.55), and β-glucosidase (90.52), which indicated of thermo-stability. Similarly, the activities (%) for CMCase (85.79), FPase (82.48), and β-glucosidase (85.92) at pH 8.5 indicated of alkaline-stability. Kinetic factors, K m and V max for endoglucanase component of fractionated CELs were 0.014 g/l and 158.23 µM glucose/min/mL, respectively. Fractionated CELs yielded activation energies (kJ/mol) of 17.933, 6.294, and 4.207 for CMCase, FPase, and β-glucosidase activities, respectively in linear thermostable Arrhenius plots. Thus, this study reports on the multipurpose CELs from an untreated agricultural residue utilizing Promicromonospora in relation to broad substrate specificity, halo-tolerance,

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Enhanced co-production of pectinase, cellulase and xylanase enzymes from Bacillus subtilis ABDR01 upon ultrasonic irradiation

Cited by 30 publications

References 28 publications

Ultrasound‐assisted fermentation for production of β‐1,3‐glucanase and chitinase by Beauveria bassiana

Ultrasound‐assisted fermentation for production of β‐1,3‐glucanase and chitinase by Beauveria bassiana

Optimization of bio-chemical degumming of Ramie fiber for improved strength & luster

Multipurpose cellulases of Promicromonospora sp. VP111, with broad substrate specificity and tolerance properties

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