Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy

Xing, Tong; Zhang, Xue; Xu, Xinglian; Li, Jiaolong; Zhang, Lin; Gao, Feng

doi:10.1002/jsfa.10641

Cited by 13 publications

(15 citation statements)

References 43 publications

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“…Therefore, these dysregulated cytokines could further lead to immune disorder and contribute to the aggravation of liver damage. This result also supports our recent finding of the systemic inflammatory response in WB myopathic broilers as implied by the elevation of circulating cytokines (Xing et al, 2021). Besides the secretion of cytokines, inflammatory cells can also produce excessive oxygen free radicals to attack host cells, leading to hepatocyte damage (Zhu et al, 2012).…”

Section: Discussionsupporting

confidence: 90%

“…Greene et al (2019) demonstrated that the circulatory oxygen homeostasis was dysregulated in WB myopathic broiler chickens as indicated by the altered pressure of blood gases and hemoglobin levels. Our recent study revealed that the inflammatory cytokines including interleukin (IL)-1β, IL-8, and tumor necrosis factor (TNF)-α were enhanced in the serum of WB affected birds (Xing et al, 2021). Furthermore, lung histopathology of WB myopathic birds exhibited occasional localized multifocal lymphoplasmacytic phlebitis and more foci of chondro-osseous metaplasia compared with the unaffected birds (Lake et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Hepatic Oxidative Stress, Apoptosis, and Inflammation in Broiler Chickens With Wooden Breast Myopathy

et al. 2021

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Wooden breast (WB) syndrome has emerged as a global myopathy in modern commercial broiler chickens, mainly affecting the pectoralis major muscle. Recent evidence suggests that WB myopathy is a systemic disease, which might be accompanied by other physiological disparities and metabolic changes. This study was conducted to systemically investigate the potential physiological changes in liver tissues as well as the possible mechanisms involved to enhance the understanding of the etiology. A total of 93 market-age Arbor Acres male broiler chickens were sampled and categorized into control (CON) and WB groups based on the evaluation of myopathic lesions. Liver samples were collected (n = 10 in each group) for histopathological evaluation and biochemical analyses. Results indicated that WB birds exhibited significantly higher plasma aspartate amino transferase, alkaline phosphatase, and gamma glutamyl transpeptidase activities. Histopathological changes in hydropic/fatty degeneration, inflammatory cell infiltration, intrahepatic hemorrhages, elevated myeloperoxidase activity, and overproduction of nitric oxide were observed in WB liver compared with CON, suggesting the occurrence of liver injury in birds affected by WB myopathy. The WB group showed increased levels of reactive oxygen species, oxidative products, as well as enhanced antioxidant capacities in the liver. These changes were associated with impaired mitochondria morphology and mitochondrial dysfunction. WB myopathy also induced mitochondria-mediated hepatic apoptosis by upregulating levels of caspases 3 and 9, altering the expressions of apoptotic B-cell lymphoma-2 family regulators, as well as increasing the release of cytochrome c. The activation of nuclear factor kappa-light-chain-enhancer of activated B cell signaling enhanced the mRNA expression of downstream inflammatory mediators, contributing to the production of inflammatory cytokines in WB liver. Combined, these findings suggest that hepatic disorders may be conjoined with WB myopathy in broiler chickens and indicating systemic physiological disparities, and other metabolic changes accompanying this myopathy need further assessment.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Hepatic Oxidative Stress, Apoptosis, and Inflammation in Broiler Chickens With Wooden Breast Myopathy

et al. 2021

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“…Dietary taurine could improve growth performance, pH value, and tenderness of broilers challenged with H 2 O 2 by activating NF-κB signaling [ 42 ]. These findings are in agreement with a recent work reporting that the NF-κB pathway was enhanced in wooden breasts [ 43 ], which was characterized by hardened areas, pale, and ridge-like bulges at the caudal end. Strikingly, WHC gradually decreased concomitant with the worsened wooden breast myopathy [ 43 ].…”

Section: Discussionsupporting

confidence: 93%

Insights into the mechanism of L-malic acid on drip loss of chicken meat under commercial conditions

Sun,

Yan,

Wang

et al. 2024

J Animal Sci Biotechnol

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Background A deterioration in the meat quality of broilers has attracted much more attention in recent years. L-malic acid (MA) is evidenced to decrease meat drip loss in broilers, but the underlying molecular mechanisms are still unclear. It’s also not sure whether the outputs obtained under experimental conditions can be obtained in a commercial condition. Here, we investigated the effects and mechanisms of dietary MA supplementation on chicken meat drip loss at large-scale rearing. Results Results showed that the growth performance and drip loss were improved by MA supplementation. Meat metabolome revealed that L-2-aminoadipic acid, β-aminoisobutyric acid, eicosapentaenoic acid, and nicotinamide, as well as amino acid metabolism pathways connected to the improvements of meat quality by MA addition. The transcriptome analysis further indicated that the effect of MA on drip loss was also related to the proper immune response, evidenced by the enhanced B cell receptor signaling pathway, NF-κB signaling pathway, TNF signaling pathway, and IL-17 signaling pathway. Conclusions We provided evidence that MA decreased chicken meat drip loss under commercial conditions. Metabolome and transcriptome revealed a comprehensive understanding of the underlying mechanisms. Together, MA could be used as a promising dietary supplement for enhancing the water-holding capacity of chicken meat.

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“…It seems likely that broiler breast muscle myopathies are associated with inflammation [262]. The NF-κB signaling pathway was found to be induced, the mRNA expression levels of downstream inflammatory mediators were increased, and TLR levels were upregulated in Pectoralis major of Wooden breast myopathy-affected broiler chickens [263]. The authors also showed that, in the serum of broilers with breast myopathies, contents of IL-1β, IL-8, and TNF-α were increased.…”

Section: Diseasesmentioning

confidence: 94%

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

2021

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Redox biology is a very quickly developing area of modern biological sciences, and roles of redox homeostasis in health and disease have recently received tremendous attention. There are a range of redox pairs in the cells/tissues responsible for redox homeostasis maintenance/regulation. In general, all redox elements are interconnected and regulated by various means, including antioxidant and vitagene networks. The redox status is responsible for maintenance of cell signaling and cell stress adaptation. Physiological roles of redox homeostasis maintenance in avian species, including poultry, have received limited attention and are poorly characterized. However, for the last 5 years, this topic attracted much attention, and a range of publications covered some related aspects. In fact, transcription factor Nrf2 was shown to be a master regulator of antioxidant defenses via activation of various vitagenes and other protective molecules to maintain redox homeostasis in cells/tissues. It was shown that Nrf2 is closely related to another transcription factor, namely, NF-κB, responsible for control of inflammation; however, its roles in poultry have not yet been characterized. Therefore, the aim of this review is to describe a current view on NF-κB functioning in poultry with a specific emphasis to its nutritional modulation under various stress conditions. In particular, on the one hand, it has been shown that, in many stress conditions in poultry, NF-κB activation can lead to increased synthesis of proinflammatory cytokines leading to systemic inflammation. On the other hand, there are a range of nutrients/supplements that can downregulate NF-κB and decrease the negative consequences of stress-related disturbances in redox homeostasis. In general, vitagene–NF-κB interactions in relation to redox balance homeostasis, immunity, and gut health in poultry production await further research.

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Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy

Cited by 13 publications

References 43 publications

Hepatic Oxidative Stress, Apoptosis, and Inflammation in Broiler Chickens With Wooden Breast Myopathy

Hepatic Oxidative Stress, Apoptosis, and Inflammation in Broiler Chickens With Wooden Breast Myopathy

Insights into the mechanism of L-malic acid on drip loss of chicken meat under commercial conditions

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

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