Enhanced cytotoxic effects of 5-aminolevulinic acid-mediated photodynamic therapy by concurrent hyperthermia in glioma spheroids

Hirschberg, Henry; Sun, Carlos; Tromberg, Bruce J.; Yeh, Alvin T.; Madsen, Steen J.

doi:10.1007/s11060-004-9161-7

Cited by 49 publications

(31 citation statements)

References 48 publications

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“…The net effect of PDT is thus to lower the activation energy of protein denaturing thus making the proteins more susceptible to thermal damage. The observation of high levels of apoptotic cell death following combined hyperthermia and PDT [36] is consistent with this hypothesis since these proteins can be found in the mitochondrial membrane and ALA-induced PpIX has significant mitochondrial localization [40].…”

Section: Pdt and Hyperthermiasupporting

confidence: 81%

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Multicell tumor spheroids in photodynamic therapy

et al. 2006

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To that end, progress has recently been made to develop a more accurate in vivo brain tumor model consisting of biopsy-derived human tumor spheroids implanted into the brains of immunodeficient rats. Finally, recent work suggests that computer simulations may prove useful to describe the growth of MCS and predict the effects of investigational therapies including PDT. Such in silico models have made a number of counterintuitive predictions that have been verified in vitro and, as such, could guide the development of improved therapeutics.

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Section: Pdt and Hyperthermiasupporting

confidence: 81%

“…ALA-PDT and hyperthermia (40-468C) have been shown to interact synergistically in both rat and human glioma spheroids if the therapies are given concurrently [36]. Neither sub-threshold fluence PDT (<25 J/cm 2 ) nor temperatures below 498C inhibited spheroid growth.…”

Section: Pdt and Hyperthermiamentioning

confidence: 99%

Multicell tumor spheroids in photodynamic therapy

et al. 2006

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“…Using a murine mammary adenocarcinoma model, Chen et al [13] showed that the combination of PDT and MHT produced a synergetic tumor response. Hirschberg et al [14] examined synergistic effects of 5-aminolevulinic acid-mediated PDT and MHT concurrently on human and rat glioma spheroids. These latter results showed that, when administered separately, PDT and HT at suboptimal levels were not very effective, however, concurrent administration of the two treatment modalities at these same levels resulted in significant spheroid growth inhibition.…”

Section: Introductionmentioning

confidence: 99%

Synergistic chemotherapy by combined moderate hyperthermia and photochemical internalization

Christie

Molina

Gonzales

et al. 2016

Biomed. Opt. Express

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Combination therapies of photochemical internalization (PCI) and moderate hyperthermia (MHT) were investigated in an in vitro system consisting of human and rat glioma spheroids. PCI using the amphiphilic photosensitizer, AlPcS2a and two anti cancer agents BLM or 5-FU were used. Spheroids were irradiated with λ = 670 nm laser light in an incubator at temperatures ranging from 37 to 44°C. For each temperature investigated, spheroids were divided into 4 groups: control, drug-only, photodynamic therapy (PDT), and PCI. PDT and PCI spheroids were exposed to radiant exposures ranging from 0.3 to 2.5 J cm −2 using an irradiance of 5 mW cm −2 . Toxicity was evaluated from spheroid growth kinetics. The combination of PCI and MHT resulted in significant increases in BLM efficacy at 44°C for both cell line derived spheroids compared to controls at 37°C over the range of radiant exposures examined. 5-FU PCI was ineffective for the human cell line at both 37 and 44°C. L. West, "Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance," Proc. Natl. Acad. Sci. U.S.A. 100(23), 13549-13554 (2003). 31. X. Huang, P. K. Jain, I. H. El-Sayed, and M. A. El-Sayed, "Determination of the minimum temperature required for selective photothermal destruction of cancer cells with the use of immunotargeted gold nanoparticles," Photochem. Photobiol. 82(2), 412-417 (2006). 32.

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“…Thus, active immunization of patients with malignant gliomas is regarded as a therapeutic strategy to promote anti-tumor response [Grauer et al, 2009;Albesiano et al, 2010;Waziri, 2010;Yang et al, 2010]. Indeed, recent findings indicate that anti-cancer treatment, such as chemotherapy, radiotherapy, hyperthermia, and high intensity focused ultrasound, can induce necrosis in tumors and boost anti-tumor immunity [Hirschberg et al, 2004;Wu et al, 2004;Heisel et al, 2008;Zitvogel et al, 2008]. Using the well established brain glioma models by implanting G422 or GL261 mouse glioma cells in mice brain, which can mimic the growth of human glioma such as the forming of spheres and invasion to the normal brain [Zhang et al, 2004], our study provides evidence to further support the anti-tumor immune strategy in the CNS.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic therapy boosts anti-glioma immunity in mice: A dependence on the activities of T cells and complement C3

Cheng

et al. 2011

J. Cell. Biochem.

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Photodynamic therapy (PDT) involves the systemic administration of a tumor-specific photosensitizer and local irradiation of visible light, can generate highly cytotoxic molecular species in the tumor and kill malignant cells directly or by shutting down the tumor microvasculature. Collectively data show that anti-tumor immunity is an important mechanism that mediates the PDT-induced tumor-destroying effects in many types of cancers. However, it is unknown whether PDT also promotes anti-tumor immunity in gliomas in the central nervous system (CNS). Here we show that the PDT generates regional and systemic anti-tumor immunity in mice with G422 gliomas in the brain. The infiltration of immune cells and the release of inflammatory factors, such as TNF-α and IFN-γ, are increased in animals with G422 gliomas following PDT when compared with those without receiving PDT. The lymphocytes that are isolated from PDT-treated mice are able to induce anti-tumor immunity in nude mice. The anti-glioma immunity fostered by PDT is inhibited in complement C3 knockout mice and the nude mice indicate the requirement of the activities of complement C3 and T cells. Thus, T cells that produce cytokines, along with complement C3, may play crucial roles in mediating PDT-induced anti-glioma responses.

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Enhanced cytotoxic effects of 5-aminolevulinic acid-mediated photodynamic therapy by concurrent hyperthermia in glioma spheroids

Cited by 49 publications

References 48 publications

Multicell tumor spheroids in photodynamic therapy

Multicell tumor spheroids in photodynamic therapy

Synergistic chemotherapy by combined moderate hyperthermia and photochemical internalization

Photodynamic therapy boosts anti-glioma immunity in mice: A dependence on the activities of T cells and complement C3

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