Multicell tumor spheroids in photodynamic therapy

Madsen, Steen J.; Sun, Carlos; Tromberg, Bruce J.; Cristini, Vittorio; Magalhães, Nzola De; Hirschberg, Henry

doi:10.1002/lsm.20350

Cited by 56 publications

(45 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spheroids are 3-D multicellular clusters that share important features with small avascular tumors, such as their histoarchitecture, their gradients of nutrients, oxygen levels, proliferation rate, and pH. 19 Hence, they are an excellent model to assess the PDT effects on NMIBC lesions in a preclinical setting.…”

Section: Introductionmentioning

confidence: 99%

Biodistribution and photodynamic effects of polyvinylpyrrolidone-hypericin using multicellular spheroids composed of normal human urothelial and T24 transitional cell carcinoma cells

et al. 2011

View full text Add to dashboard Cite

Polyvinylpyrrolidone (PVP)-hypericin is a potent photosensitizer that is used in the urological clinic to photodiagnose with high-sensitivity nonmuscle invasive bladder cancer (NMIBC). We examined the differential accumulation and therapeutic effects of PVP-hypericin using spheroids composed of a human urothelial cell carcinoma cell line (T24) and normal human urothelial (NHU) cells. The in vitro biodistribution was assessed using fluorescence image analysis of 5-μm cryostat sections of spheroids that were incubated with PVP-hypericin. The results show that PVP-hypericin accumulated to a much higher extent in T24 spheroids as compared to NHU spheroids, thereby reproducing the clinical situation. Subsequently, spheroids were exposed to different PDT regimes with a light dose ranging from 0.3 to 18J/cm 2 . When using low fluence rates, only minor differences in cell survival were seen between normal and malignant spheroids. High light fluence rates induced a substantial difference in cell survival between the two spheroid types, killing ∼80% of the cells present in the T24 spheroids. It was concluded that further in vivo experiments are required to fully evaluate the potential of PVP-hypericin as a phototherapeutic for NMIBC, focusing on the combination of the compound with methods that enhance the oxygenation of the urothelium. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

show abstract

Section: Introductionmentioning

confidence: 99%

Biodistribution and photodynamic effects of polyvinylpyrrolidone-hypericin using multicellular spheroids composed of normal human urothelial and T24 transitional cell carcinoma cells

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Compared to F98 monolayer cultures, the spheroids were much more resistant to the toxic effects of the drug. This difference between 2D and 3D cell cultures has been established previously [19]. Tumor spheroids mimic in vivo tumors in their microenvironment and, as such, are superior to monolayer cultures for this type of in vitro study as they represent a bridge to in vivo animal experiments.…”

Section: Resultsmentioning

confidence: 97%

“…Nevertheless, the spheroids were still in a growth phase and eventually reached control values when monitored for a longer interval. Comparable spheroid models have been employed in several of our previous studies examining the effects of photodynamic and photothermal therapies on spheroid growth [8,19]. Regardless of treatment modality, insufficient doses often result in growth delay rather than growth cessation as demonstrated in Fig.…”

Section: Resultsmentioning

confidence: 99%

Photochemical internalization enhanced macrophage delivered chemotherapy

Shin

Christie

et al. 2018

Photodiagnosis and Photodynamic Therapy

Self Cite

View full text Add to dashboard Cite

Background Macrophage (Ma) vectorization of chemotherapeutic drugs has the advantage for cancer therapy in that it can actively target and maintain an elevated concentration of drugs at the tumor site, preventing their spread into healthy tissue. A potential drawback is the inability to deliver a sufficient number of drug-loaded Ma into the tumor, thus limiting the amount of active drug delivered. This study examined the ability of photochemical internalization (PCI) to enhance the efficacy of released drug by Ma transport. Methods Tumor spheroids consisting of either F98 rat glioma cells or F98 cells combined with a subpopulation of empty or doxorubicin (DOX)-loaded mouse Ma (RAW264.7) were used as in vitro tumor models. PCI was performed with the photosensitizer AlPcS2a and laser irradiation at 670 nm. Results RAW264.7 Ma pulsed with DOX released the majority of the incorporated DOX within two hours of incubation. PCI significantly increased the toxicity of DOX either as pure drug or derived from monolayers of DOX-loaded Ma. Significant growth inhibition of hybrid spheroids was also observed with PCI even at subpopulations of DOX-loaded Ma as low as 11% of the total initial hybrid spheroid cell number. Conclusion Results show that RAW264.7 Ma, pulsed with DOX, could effectively incorporate and release DOX. PCI significantly increased the ability of both free and Ma-released DOX to inhibit the growth of tumor spheroids in vitro. The growth of F98+DOX loaded Ma hybrid spheroids were synergistically reduced by PCI, compared to either photodynamic therapy or released DOX acting alone.

show abstract

“…They display many characteristics similar to tumor cells in vivo (for a review see Ref. [43]). We have investigated Mo infiltrating into the spheroid as a function of time is shown graphically in Figure 4C and reaches saturation after 12 h of co-incubation in this model.…”

Section: Mo Ns Migration In Human Glioma Spheroids and Ma Spio Into Ratmentioning

confidence: 99%

Macrophages As Nanoparticle Delivery Vectors for Photothermal Therapy of Brain Tumors

et al. 2015

Self Cite

View full text Add to dashboard Cite

Certain types of stem and immune cells, which have an innate ability to target and infiltrate tumors, can be utilized as vectors to deliver several types of anticancer agents. In particular monocytes have the advantage of carrying relatively large payloads of therapeutic nanomaterials, can be patient derived in large numbers and are able to actively infiltrate tumors despite many barriers often present in the microenvironment. Monocytes can selectively cross the compromised blood-brain barrier surrounding brain tumors and are known to actively migrate to hypoxic tumor regions. Of particular interest is the observation that, following near-infrared exposure of tumors containing gold-nanoshell-loaded macrophages, sufficient hyperthermia can be generated to suppress tumor growth. Collectively, these findings demonstrate the potential of monocytes as nanoparticle delivery vectors for several types of site specific light-based cancer therapies.

show abstract

Multicell tumor spheroids in photodynamic therapy

Cited by 56 publications

References 66 publications

Biodistribution and photodynamic effects of polyvinylpyrrolidone-hypericin using multicellular spheroids composed of normal human urothelial and T24 transitional cell carcinoma cells

Biodistribution and photodynamic effects of polyvinylpyrrolidone-hypericin using multicellular spheroids composed of normal human urothelial and T24 transitional cell carcinoma cells

Photochemical internalization enhanced macrophage delivered chemotherapy

Macrophages As Nanoparticle Delivery Vectors for Photothermal Therapy of Brain Tumors

Contact Info

Product

Resources

About