Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) Vesicles

Abstract

Span 80 (sorbitan monooleate) vesicles behaved differently from conventional phospholipid vesicles (liposomes) because the former had a more fluid interface. After doxorubicin hydrochloride (DOX) was encapsulated into the Span 80 vesicle (loading efficiency: 63 %), DOX-loaded Span 80 vesicles (DVs) were thereafter added to Colon 26 cells. It was suggested, from the flow cytometric analysis and confocal laser microscopic observation, that DVs directly deliver DOX into the cytoplasm of Colon 26 cells. DVs showed the different delivery manner from the DOX-loaded liposomes (DLs). It is considered that the difference of delivery manner between DVs and DLs resulted in the difference of cytotoxicity (IC50); i.e. IC50 values for DVs and DLs were 5 and > 30 μM, respectively. The results obtained herein would give the fundamental findings which can contribute to the improvement of formulation of conventional liposome-based carrier and its cytotoxicity.

Development of tumor-specific caffeine-potentiated chemotherapy using a novel drug delivery system with Span 80 nano-vesicles

“…Our findings suggest that the higher tumor selectivity of Span 80 vesicles may be associated with their pegylation as well as high fluidity and flexibility, which result in higher vascular permeability and tendency to fuse with the instable cell membrane of the tumors ( 27 ). Indeed, a cell fusion model of Span 80 vesicles has been recently reported ( 11 ).…”

Passive targeting of phosphatiosomes increases rolipram delivery to the lungs for treatment of acute lung injury: An animal study

“…The increased lung uptake thus can be attained. The addition of phospholipids in nanosystems elevates the interface rigidity and biocompatibility [11,12]. It is hypothesized that incorporation of PC would target the niosomes to the lungs and minimize the propensity for liver and spleen uptake, making the nanovesicles more attractive for drug delivery.…”

Development of Tumor-Specific Caffeine-Potentiated Chemotherapy Using Span 80 Nano-Vesicles DDS

“…The results of our study indicated that higher vascular permeability and inclination to fuse with the instable cell membrane of the tumors based on high fluidity and flexibility as well as pegylation could result in the higher tumor selectivity of Span 80 vesicles [52]. Recently, a cell fusion model using Span 80 vesicles has been reported [27].…”

“…The next-generation liposomes with membrane-bound-targeting molecules have also been under development. An anticarcinoma application of Span 80 vesicles containing doxorubicin with or without membrane-bound-targeting molecule was recently reported [52]. As described above, Span 80 has favorable physicochemical properties; moreover, it also confirmed risk-free information because it has been already used as a stabilizer for injected drugs.…”