Enhanced determination of fertility hormones in saliva at disposable immunosensing platforms using a custom designed field-portable dual potentiostat

Quick and Cost-Effective Estimation of Vitamin C in Multifruit Juices Using Voltammetric Methods

“…An improved version of our previously published potentiostat [19] has been designed and developed to carry out the determination of AA in multifruit juices ( Figure 1). Furthermore, note that the authors have a wide experience in this type of devices, as is corroborated in the works [23,24]. The new version of the device incorporates the following advantages: (i) full portability, allowing measurements at the industrial plant where juices or other foodstuffs are being manufactured; (ii) better isolation to reduce electromagnetic noise; and (iii) easy-to-use software (including user interface), which allows the user to put a controlled volume of the substance to be analyzed into the sensor and then simply activate a button on the touchscreen to obtain the AA level of the sample.…”

Multiplexed Determination of Fertility‐related Hormones in Saliva Using Amperometric Immunosensing

“…While anti‐E2‐Protein G‐MBs were directly incubated with 25 μL of untreated saliva sample, since the protocol for FSH determination involves the previous formation of sandwich immunocomplexes in homogeneous solution and their subsequent capture on MBs, the NA‐MBs were incubated with 125 μL of a mixture solution containing 12.5 μL of untreated saliva (i. e. a 10‐fold diluted saliva). Moreover, the high affinity of human IgGs for Protein G can explain the matrix effect and the smaller slope value observed for the determination of E2 due to the displacement of the anti‐E2 antibody from the Protein G‐MBs by the salivary IgGs [26, 27].…”

Understanding and Assisting Reproduction in Wildlife Species Using Microfluidics

“…To predict ovulation, other strategies have been proposed for quantitative and more sensitive analysis of different hormones, but they have only been validated in the laboratory. Using electrochemical detection with signal amplification, luteinizing hormone (LH) and progesterone (P4) have been quantified in saliva [13], and β-estradiol in serum [14], using sandwich-based or competitive immunoassays. In a more integrated platform equipped with a dedicated cartridge containing all necessary buffers and pre-loaded with all reagents, LH, follicle-stimulating hormone (FSH), β-hCG, and prostaglandin were simultaneously analyzed in serum using a sandwich immunoassay followed by quantitative fluorescence detection [15].…”