Enhanced effects of ferulic acid against the harmful side effects of chemotherapy in colon cancer: docking and in vivo study

Abstract

Background Colorectal cancer is considered a potential causative agent of morbidity and death, making it a particularly dangerous malignancy. The current study aims to assess the efficacy of ferulic acid (FA) to attenuate the harmful side effect of 5-fluorouracil (5FU) in colon cancer tissues induced by 1,2-dimethylhydrazine (DMH). Results Regarding the colon tissues of male Wistar-albino rats (Rattus norvegicus), combined FA and 5FU showed the approximately normal structure of mucosa. The treated groups showed a remarkable reduction in Ki67, Ck20, and an elevation in caspase-3 and P53. There was significant upregulation of P53 in both 5FU and combined FA–5FU groups (p < 0.001 and p < 0.00001, respectively). Conclusions The present results revealed a potential role of the combined therapy by 5FU and FA in the suppression of colon cancer induced by DMH by upregulation of apoptosis with the clear effect of FA in attenuating the side effects of 5FU on the normal cells.

Hesperetin and Capecitabine Abate 1,2 Dimethylhydrazine-Induced Colon Carcinogenesis in Wistar Rats via Suppressing Oxidative Stress and Enhancing Antioxidant, Anti-Inflammatory and Apoptotic Actions

“…The enhanced malignancy of several major human cancers, including CRC, is associated with an increase in p53 accumulation in the cytoplasm, where the p53 protein is not functional [109]. When compared to normal control rats, those given DMH, in the current study, had colonic cancerous lesions and a significant decrease in the level of colonic p53, which was in agreement with the findings of Gadelmawla et al [110]. In the present study, the expression of p53 in the colons of the rats given DMH and treated with HES and CAP was high, particularly in the group administered HES.…”

Mitigating effect of ferulic acid on di-(2-ethylhexyl) phthalate-induced neurocognitive dysfunction in male rats with a comprehensive in silico survey

“…Therefore, it is highly favorable for FA to follow Lipinski’s rule of five. Moreover, a docking study of FA was employed for the prediction of binding mode and to understand its inhibition ability on caspase-3 (PDB code 1RHR) (Gadelmawla et al 2022 ) and NO synthase (PDB code 4NOS) (Fischmann et al 1999 ).…”

“…Molecular docking was done according to the reported procedures (Abdelnaby et al 2022 ; El-Dydamony et al 2022 ) using Discovery Studio 4.1 software (Accelrys, Inc., San Diego, CA, USA). The X-ray 3D structures of caspase-3 (PDB ID 1RHR) (Gadelmawla et al 2022 ) and NO synthase (PDB ID 4NOS) (Fischmann et al 1999 ) were obtained from the PDB site ( http://www.rscb.org/pdb ) and prepared for docking by cleaning the protein and fixing missing chains. The CHARMm forcefield was applied, and energy was minimized.…”

“…The best ten poses were studied, and the one with the best score and orientation was chosen. Our ligands were joined with the reported amino acid residues (Gadelmawla et al 2022 ).…”

Dual pH- And Temperature-Responsive Poly(Dimethylaminoethyl Methacrylate)-Coated Mesoporous Silica Nanoparticles as a Smart Drug Delivery System

“…After the growth of nanotechnology in the recent years, the use of nanoparticles with the ability of targeting the tumors has been developed 19 . These nanoparticles accumulate in tumor tissue to reduce the side effects of chemotherapy 20 . There are many bene ts of using nanoparticles in chemotherapy, some of which are 1) preventing from and protecting the destruction of drug in the body, 2) enlarging the amount of drug delivered to the tumor, 3) targeting the tumor precisely, 4) and controllability of all stages of treatment 21 .…”