Enhanced Electrochemiluminescence Detection for Hydrogen Peroxide Using Peroxidase-Mimetic Fe/N-Doped Porous Carbon

Abstract

Detecting hydrogen peroxide (H 2 O 2 ) is obviously indispensable on account of its low toxicity and wide application, and it is still a challenge for developing highly sensitive and selective electrochemiluminescence (ECL) sensors based on the noble metal-free electrode. Hence, we designed an accurate ECL sensor for detecting trace H 2 O 2 based on N-doped porous carbon containing Fe (Fe/N-C) nanocomposites, which were successfully synthesized by pyrolysis of NH 2 -MIL-101(Fe) metal-organic framework (MOF). Fe/N-C nanocomposite significantly improved the electron transfer process and enhanced the catalytic activity of ECL sensor for trace H 2 O 2 detection. Fe/N-C nanocomposite significantly contributed in the conversion of H 2 O 2 to superoxide radical, followed by the oxidation of luminal which enhanced the electrochemical luminescence signal. Predictably, the ECL sensing strategy at Fe/N-C nanocomposite electrode showed a satisfying linear range from 1 nM to 300 nM for H 2 O 2 detection with limit of detection (LOD) as 0.93 nM (signal to noise ratio (S/N) = 3). Also, the ECL sensor was used to detect H 2 O 2 in real water samples and renal epithelial 293T cells, which justified great promise for the practical ECL detection of H 2 O 2 .

3D Flower-Like MoS2 Nanomaterial as Signal-Promoter of PTC-PEI/S2O8 2− System for Fabricating a Sensitive Electrochemiluminescence Methotrexate Sensor

“…Electrochemiluminescence (ECL) technique as a powerful analytical strategy has received tremendous attention in biosensor application owing to its high analytical sensitivity, low background signal and controllability. [1][2][3][4] As a classic ECL luminophores, Perylene derivatives exihibit excellent chemical stability, high luminescent efficiency and admirable membrane-forming properties. [5][6][7][8] However, the ECL emitting of perylene derivatives with S 2 O 8 2− as coreactant is not strong enough to realize trace determination, thus greatly limiting the application of perylene derivatives in ECL biosensor field.…”

Use of an Artificial Miniaturized Enzyme in Hydrogen Peroxide Detection by Chemiluminescence

“…Moreover, the presence of 10 ppm of a well-known reducing aromatic compound did not influence the assay outcome, demonstrating the usability of FeMC6*a for this kind of application in hydrogen peroxide sensing. Future efforts will be devoted to enhance the performances of FeMC6*a in luminol-based H 2 O 2 determination either by using enhancers or immobilization onto solid supports [ 16 , 19 , 23 , 25 , 30 , 31 , 64 ], and to the construction of a standalone sensor device. Process control and quality assessment of reclaimed water may be further improved by developing efficient multi-purpose devices [ 4 , 28 ].…”

“…Many bio-based assays have been developed with potential food, clinical, and biotechnological applications [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. Different techniques may be coupled to these assays, such as spectrophotometry [ 9 ], fluorimetry [ 10 , 11 ], electrochemistry [ 12 , 13 , 14 , 15 , 16 , 17 ], and chemiluminescence (CL) [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ], giving rise to a wide spectrum of sensitivities and variable ranges of detection. CL offers several advantages, such as the use of very sensitive and miniaturized detectors, from lab bench PMT detectors to smartphone CCD cameras, and the almost complete absence of background signal [ 8...…”

“…Many bio-based assays have been developed with potential food, clinical, and biotechnological applications . Different techniques may be coupled to these assays, such as spectrophotometry [9], fluorimetry [10,11], electrochemistry [12][13][14][15][16][17], and chemiluminescence (CL) [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33], giving rise to a wide spectrum of sensitivities and variable ranges of detection. CL offers several advantages, such as the use of very a wide spectrum of sensitivities and variable ranges of detection.…”

Nitrogen-Rich Precursors Assisted Synthesis of Metal-Organic Framework-Derived Nanostructures as Bifunctional Catalysts for Electrochemical Sensing and Oxygen Reduction Reaction

“…22,23 As convenient self-templated precursors, metal-organic frameworks (MOFs) can be pyrolyzed to obtain M-NC structures, which is a typical approach to produce such porous materials. [24][25][26][27] However, it is difficult to realize the regulation of the species and locations of nitrogen when only pyrolyzing pure MOFs under the same condition.…”