Enhanced enzymatic activity of phenylalanine dehydrogenase caused by cyclodextrins

Gubica, Tomasz; Pałka, Katarzyna; Szeleszczuk, Łukasz; Kańska, Marianna

doi:10.1016/j.molcatb.2015.05.004

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…is employed to convert Phe into phenylpyruvate and stoichiometrically reduce NAD + into NADH (Scheme 1a). The pH value favorable for the Phe conversion and NADH formation is 10.5, 32–35 which is higher than that suitable for NTR catalyzing RF in the fluorescence readout process (pH 7.4). 31,36 A simple solution adopting different buffers for these cascade reactions is proposed to resolve this situation.…”

Section: Resultsmentioning

confidence: 87%

An enzyme cascade fluorescence-based assay for the quantification of phenylalanine in serum

Meng

Chen

et al. 2022

Analyst

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Section: Resultsmentioning

confidence: 87%

An enzyme cascade fluorescence-based assay for the quantification of phenylalanine in serum

Meng

Chen

et al. 2022

Analyst

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“…The most recent studies make extensive use of the powerful catalytical properties of enzymes in supramolecular structures to produce biosensors. In this context, different strategies for the immobilization of enzymes related to the l -phenylalanine metabolism have been proposed, with the phenylalanine dehydrogenase enzyme (PheDH) as the primary candidate. − This enzyme belongs to the oxidoreductase class of enzymes and is responsible for the reversible reaction of oxidative deamination of l -phenylalanine to phenylpyruvate in the presence of NAD + as a cofactor …”

Section: Introductionmentioning

confidence: 99%

Surface Chemistry Studies on the Formation of Mixed Stearic Acid/Phenylalanine Dehydrogenase Langmuir and Langmuir–Blodgett Films

et al. 2021

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This work investigates the physicochemical properties of mixed stearic acid (HSt)/phenylalanine dehydrogenase enzyme (PheDH) Langmuir films and their immobilization onto solid supports as Langmuir−Blodgett (LB) films. PheDH from the aqueous subphase enters the surfactant matrix up to an exclusion surface pressure of 25.3 mN/m, leading to the formation of stable and highly condensed mixed Langmuir monolayers. Hydrophobic interactions between the enzyme and HSt nonpolar groups tuned the secondary structure of PheDH, evidenced by the presence of βsheet structures as demonstrated by infrared and circular dichroism spectra. The floating monolayers were successfully transferred to solid quartz supports, yielding Y-type LB films, and then characterized employing fluorescence, circular dichroism, and microscopic techniques, which indicated that PheDH was co-immobilized with HSt proportionally to the number of transferred layers. The enzyme fluidized the HSt monolayers, reducing their maximum dipoles when condensed to their maximum, and disorganized the alkyl chains of the fatty acid, as detected with infrared spectroscopy. The stability of the mixed floating monolayers enabled their transfer to solid supports as LB films, which is important for producing optical and electrochemical sensors for phenylalanine whose molecular architecture can be controlled with precision.

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“…Many studies aimed at developing new PKU monitoring strategies, relying on several different approaches, have been published. These include enzyme-based (Gubica et al 2015;Mangombo et al 2013;Naghib, Rabiee, Omidinia, and Khoshkenar 2012;Naghib, Rabiee, Omidinia, Khoshkenara, et al 2012;Omidinia 2014a, 2014b;Omidinia, Khanehzar, et al 2013;Omidinia, Shadjou, and Hasanzadeh 2014b;Villalonga et al 2007Villalonga et al , 2008Weiss et al 2007;Zhuo Wang et al 2005), aptamer-based (Idili et al 2021), (Omidinia, Shadjou, and Hasanzadeh 2014a) and nanomaterials (Hasanzadeh et al 2009(Hasanzadeh et al , 2012Hasanzadeh, Shadjou, and Omidinia 2013;Omidinia, Shadjou, and Hasanzadeh 2013;Wu et al 2017) based electrochemical sensors, as well as immune sensors, (Kubota, Mizukoshi, and Miyano 2013) quartz crystal microbalance (QCM), (Cho et al 2017;Emir Diltemiz et al 2017;Mirmohseni, Shojaei, and Farbodi 2008) extended field gate field effect transistor (Iskierko et al 2017) and photometricbased strategies. Some of the principle challenges facing the development of an effective biosensor for PKU monitoring include (1) having a linear range which covers the 120 mol/L and 360 mol/L key threshold values for L-Phe described previously, (2) being able to detect L-Phe in patient-derived samples with low chemical interference (e.g.…”

Section: Introductionmentioning

confidence: 99%

A preclinical biosensor for detecting phenylalanine photometrically in plasma and whole blood samples

Rodriguez

Valles

Romeo

et al. 2021

Preprint

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Phenylketonuria (PKU) is a metabolic disease resulting from a deficiency in the enzyme phenylalanine hydroxylase, increasing L-Phenylalanine (L-Phe) values in the blood and consequently in the brain. If untreated, PKU leads to neurological damage, which can be prevented by following a diet low in L-Phe. Thus, early detection of PKU in newborns is essential. The disease’s screening and monitoring are centralized in reference centers, which require specialized equipment. However, using these techniques, sample treatment is required before the analysis, and trained personnel must perform and interpret the results. In this work, we present an enzyme-based photometric strategy to measure blood L-Phe. An enzymatic mixture, selective for L-Phe, is immobilized on an UV transparent well, and the amount of consumed co-factor is monitored at 340 nm. Standard plasma and whole blood samples were chosen to pre-validate the sensor. The samples were spiked with an increasing amount of L-Phe, accurately discriminating between physiological and pathological L-Phe concentrations. The strategy can be easily extended to analyzing other samples, such as urine or sweat. The proposed photometric system allows to analyze up to 16 samples simultaneously within a matter of hours. The measurements are relatively fast, versatile, cost-effective, and easy to carry out.

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Enhanced enzymatic activity of phenylalanine dehydrogenase caused by cyclodextrins

Cited by 7 publications

References 32 publications

An enzyme cascade fluorescence-based assay for the quantification of phenylalanine in serum

An enzyme cascade fluorescence-based assay for the quantification of phenylalanine in serum

Surface Chemistry Studies on the Formation of Mixed Stearic Acid/Phenylalanine Dehydrogenase Langmuir and Langmuir–Blodgett Films

A preclinical biosensor for detecting phenylalanine photometrically in plasma and whole blood samples

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