Enhanced expression of neuronal nitric oxide synthase and phospholipase C-γ1 in regenerating murine neuronal cells by pulsed electromagnetic field

Kim, Sung Sook; Shin, Hye Jung; Eom, Dae-Woon; Huh, Jimi; Woo, Yeongju; Kim, Hyo-Kyung; Ryu, Sung Ho; Suh, Pann Gill; Kim, Moon Jung; Kim, Jee Yun; Koo, Tae Wan; Cho, Yoon Hee; Chung, Sung Min

doi:10.1038/emm.2002.8

Cited by 38 publications

(23 citation statements)

References 2 publications

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“…Other electromagnetic signals have also been reported to increase chondrocyte cell number but not with such short exposures. [18][19][20] The results of this study also suggest that release of NO is part of the biologic pathway involved in increased DNA content of chondrocyte cultures following PEF treatment. NO increased within the 30-min PEF exposure and blocking NOS with L-NAME blocked both short-term (30 min) NO release and long-term (72 h) increase in DNA content of chondrocyte cultures.…”

supporting

confidence: 59%

A pulsing electric field (PEF) increases human chondrocyte proliferation through a transduction pathway involving nitric oxide signaling

Fitzsimmons¹,

Gordon²,

Kronberg³

et al. 2008

Journal Orthopaedic Research

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A potential treatment modality for joint pain due to cartilage degradation is electromagnetic fields (EMF) that can be delivered, noninvasively, to chondrocytes buried within cartilage. A pulsed EMF in clinical use for recalcitrant bone fracture healing has been modified to be delivered as a pulsed electric field (PEF) through capacitive coupling. It was the objective of this study to determine whether the PEF signal could have a direct effect on chondrocytes in vitro. This study shows that a 30-min PEF treatment can increase DNA content of chondrocyte monolayer by approximately 150% at 72 h poststimulus. Studies intended to explore the biological mechanism showed this PEF signal increased nitric oxide measured in culture medium and cGMP measured in cell extract within the 30-min exposure period. Increasing calcium in the culture media or adding the calcium ionophore A23187, without PEF treatment, also significantly increased short-term nitric oxide production. The inhibitor W7, which blocks calcium/calmodulin, prevented the PEF-stimulated increase in both nitric oxide and cGMP. The inhibitor L-NAME, which blocks nitric oxide synthase, prevented the PEF-stimulated increase in nitric oxide, cGMP, and DNA content. An inhibitor of guanylate cyclase (LY83583) blocked the PEF-stimulated increase in cGMP and DNA content. A nitric oxide donor, when present for only 30 min, increased DNA content 72 h later. Taken together, these results suggest the transduction pathway for PEFstimulated chondrocyte proliferation involves nitric oxide and the production of nitric oxide may be the result of a cascade that involves calcium, calmodulin, and cGMP production. ß

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supporting

confidence: 59%

A pulsing electric field (PEF) increases human chondrocyte proliferation through a transduction pathway involving nitric oxide signaling

Fitzsimmons¹,

Gordon²,

Kronberg³

et al. 2008

Journal Orthopaedic Research

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“…28 PEMF augmentation of both NO levels and NO synthases has been reported in MC3T3-E1 cells and in murine, neuronal cells respectively. 29,30 Like pulsed electromagnetic fields, pulsed ultrasound fields have also been shown to induce NO production in osteoblastic and endothelial cells. 31,32 Our findings indicated an enhancement of eNOS phosphorylation on the activating residue serine 1179 in response to as little as 10 min of PEMF exposure (Fig.…”

Section: Discussionmentioning

confidence: 99%

Pulsed electromagnetic fields rapidly modulate intracellular signaling events in osteoblastic cells: Comparison to parathyroid hormone and insulin

Schnoke

Midura

2007

Journal Orthopaedic Research

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Pulsed electromagnetic field (PEMF) devices are approved for the healing of bone nonunions, but there is a lack of understanding as to their mechanism of action at the cell and molecular level. Intermittent parathyroid hormone (PTH) therapy is currently utilized for treatment of osteoporosis, and is also being investigated for the purpose of augmenting fracture healing. Insulin and IGF-1 are also thought to play important anabolic roles in osteogenesis. In this report, signaling pathways activated by acute PTH or insulin treatments were compared to those activated by PEMF treatment in osteoblast-like cells. Some signaling molecules like the extracellular response kinases 1/2 (Erk1/2) and the cAMP response element binding protein (CREB) were activated by insulin and PTH, respectively, but not by PEMF treatment. Other signaling molecules like the insulin receptor substrate-1 (IRS-1), the S6 ribosomal subunit kinase, and the endothelial nitric oxide synthase (eNOS) were phosphorylated by PTH, insulin, and PEMF to the same relative extent and within the same time frame. IRS-1, eNOS, and S6 have been implicated in bone anabolism, and our results suggest that the anabolic effects of PEMF may be mediated, in part, through the activation of these proteins. ß

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“…It is also shown that NO synthase is supposed to be stimulated by the EMF exposure (Jeong et al, 2006;Kim et al, 2002;Seaman et al, 2002). This compound may be involved in the stimulation of lipid peroxidation indirectly.…”

Section: Elf Effect On Oxidant-antioxidant Balancementioning

confidence: 99%

Epinephrine, DNA integrity and oxidative stress in workers exposed to extremely low-frequency electromagnetic fields (ELF-EMFs) at 132 kV substations

Tiwari

Lakshmi

Bhargava³

et al. 2014

Electromagnetic Biology and Medicine

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There is apprehension about widespread use of electrical and electromagnetic gadgets which are supposed to emit electromagnetic radiations. Reports are controversy. These electromagnetic fields (EMFs) have considerable effect on endocrine system of exposed subjects. This study was focused to assess the possible bioeffects of extremely low-frequency (ELF)-EMFs on epinephrine level, DNA damage and oxidative stress in subjects occupationally exposed to 132 kV high-voltage substations. The blood sample of 142 exposed subjects and 151 non-exposed individuals was analyzed. Plasma epinephrine was measured by enzyme-linked immunosorbent assay, DNA damage was studied by alkaline comet assay along with oxidative stress. Epinephrine levels of sub-groups showed mean concentration of 75.22 ± 1.46, 64.43 ± 8.26 and 48.47 ± 4.97 for high, medium and low exposed groups, respectively. DNA damage ranged between 1.69 µm and 9.91 µm. The oxidative stress levels showed significant increase. The individuals employed in the live-line procedures were found to be vulnerable for EM stress with altered epinephrine concentrations, DNA damage and increased oxidative stress.

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Enhanced expression of neuronal nitric oxide synthase and phospholipase C-γ1 in regenerating murine neuronal cells by pulsed electromagnetic field

Cited by 38 publications

References 2 publications

A pulsing electric field (PEF) increases human chondrocyte proliferation through a transduction pathway involving nitric oxide signaling

A pulsing electric field (PEF) increases human chondrocyte proliferation through a transduction pathway involving nitric oxide signaling

Pulsed electromagnetic fields rapidly modulate intracellular signaling events in osteoblastic cells: Comparison to parathyroid hormone and insulin

Epinephrine, DNA integrity and oxidative stress in workers exposed to extremely low-frequency electromagnetic fields (ELF-EMFs) at 132 kV substations

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