Enhanced expression of selectins in human skin wounds

Dreßler, Jan; Bachmann, Lutz; Koch, Richard; Müller, Erich

doi:10.1007/s004140050196

Cited by 54 publications

(39 citation statements)

References 23 publications

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“…Wound age determination is closely involved in the pathophysiology of the wound healing process. With advances in biochemical and immunohistochemical techniques, several markers have been applied to wound age determination [3,4,5,6,7,8] and immunohistochemical analysis is now exclusively utilized for wound age determination [9,10,11,12,13,14,15,16,17,18,19,20,21]. VEGF has been characterized as an angiogenic growth factor from media conditioned by bovine pituitary folliculostellate cells.…”

Section: Discussionmentioning

confidence: 99%

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Forensic application of VEGF expression to skin wound age determination

et al. 2004

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An immunohistochemical study combined with morphometry was carried out to examine the time-dependent expression of vascular endothelial growth factor (VEGF) using 53 human skin wounds with different wound ages (groups I: 0-12 h, II: 1-4 days, III: 7-14 days and IV: 17-21 days). In the human wound specimens aged 4-12 h, neutrophils recruited at the wound showed no positive signals for VEGF. With an increase in wound ages of > or =7 days, granulation tissue and angiogenesis were observed, with the migration of macrophages and fibroblasts of which the cytoplasm expressed VEGF-positive reactions. Morphometrically, the average VEGF-positive ratio was highest in group III, followed by that of group IV. In groups III and IV, 13 out of 26 wound samples had VEGF-positive ratios of more than 50%. However, all of the wound samples in groups I and II showed VEGF-positive ratios of less than 50%. With regard to the practical applicability and forensic validity, these observations suggest that a VEGF-positive ratio of more than 50% possibly indicates a wound age of 7 days or more.

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Section: Discussionmentioning

confidence: 99%

“…Thus, many studies on forensic wound age determination have been performed [3,4,5,6,7,8,9,10]. Recently, biological substances such as growth factors, cytokines, and adhesion molecules have become useful markers for wound age determination [11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30].…”

Section: Introductionmentioning

confidence: 99%

Forensic application of VEGF expression to skin wound age determination

et al. 2004

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“…In forensic practice, it is most important to determine the post-infliction intervals of skin wounds or brain injuries, because forensic pathologists are required to judge how wounds or injuries are related to the cause of death [3][4][5][6][7][8][9][10][11][12]. Similarly, in cases of pulmonary thromboembolism, forensic pathologists have to determine the age of a thrombus in order to judge the causal relationship between the venous thrombus and trauma.…”

Section: Introductionmentioning

confidence: 99%

Time-dependent appearance of intrathrombus neutrophils and macrophages in a stasis-induced deep vein thrombosis model and its application to thrombus age determination

et al. 2009

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We immunohistochemically examined neutrophils and macrophages in venous thrombi, which developed in the ligation of the inferior vena cava (IVC). Myeloperoxidase (MPO)-positive neutrophils and F4/80-positive macrophages were detected in the whole course of thrombi after IVC ligation. Morphometrically, the number of neutrophils was greatest at 1 day after IVC ligation and, thereafter, gradually decreased with an increase of the post-ligation interval. In contrast, the number of macrophages peaked at 7 days after ligation. The number of intrathrombus neutrophils was significantly higher than that of intrathrombus macrophages at 1 and 3 days, and the average ratios of neutrophils to macrophages (N/M ratios) were 6.8 +/- 1.1 (4.8-9.0) and 2.5 +/- 0.4 (1.7-4.2) at 1 and 3 days, respectively. After more than 5 days, all samples had N/M ratios of <2.0 (0.2-1.4). These observations suggest that an N/M ratio of >2.0 indicates a thrombus age of 1-3 days. To differentiate between 1- and 3-day-old thrombi, an N/M ratio markedly exceeding 5.0 strongly indicates an age of 1 day. Furthermore, an N/M ratio of 1.0 or less probably indicates an age of more than 5 days. The present study demonstrated that the immunohistochemical detection of intrathrombus neutrophils and macrophages was suitable to determine the age of venous thrombi.

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“…As the percentages of polymorphonuclear neutrophils, mononuclear cells, and fibroblastic cells in injured zones reportedly change over time [18,[20][21][22], they may have potential use in estimating wound age. Some studies have reported a positive correlation between an early wound stage and markers' levelseven as early as 1998 when Dressler et al [23] observed strongly positive immunohistochemical reactions for P-selectin 3 min after wound creation. The above findings will be useful in future studies.…”

Section: Morphological Analysismentioning

confidence: 99%

Vitality and wound-age estimation in forensic pathology: review and future prospects

Bai

et al. 2018

Forensic Sciences Research

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Determining the age of a wound is challenging in forensic pathology, but it can contribute to the reconstruction of crime scenes and lead to arrest of suspects. Forensic scholars have tended to focus on evaluating wound vitality and determining the time elapsed since the wound was sustained. Recent progress in forensic techniques, particularly high-throughput analyses, has enabled evaluation of materials at the cellular and molecular levels, as well as simultaneous assessment of multiple markers. This paper provides an update on wound-age estimation in forensic pathology, summarizes the recent literature, and considers useful additional information provided by each marker. Finally, the future prospects for estimating wound age in forensic practise are discussed with the hope of providing something useful for further study.

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Enhanced expression of selectins in human skin wounds

Cited by 54 publications

References 23 publications

Forensic application of VEGF expression to skin wound age determination

Forensic application of VEGF expression to skin wound age determination

Time-dependent appearance of intrathrombus neutrophils and macrophages in a stasis-induced deep vein thrombosis model and its application to thrombus age determination

Vitality and wound-age estimation in forensic pathology: review and future prospects

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