Enhanced expression of the Epithelial Sodium Channel in neutrophils from hypertensive patients

Reus-Chavarría, Erika; Martínez‐Vieyra, Ivette; Salinas-Nolasco, Cristina; Chávez-Piña, Araceli Evangelina; Méndez-Méndez, Juan Vicente; López-Villegas, Edgar Óliver; Sosa‐Peinado, Alejandro; Cerecedo, Doris

doi:10.1016/j.bbamem.2018.11.003

Cited by 20 publications

(13 citation statements)

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“…This widespread expression profile of ENaC suggests its potential role under physiological and pathological conditions. Indeed, the abnormalities of ENaC in the central nervous system (Van Huysse et al, 2012), immune system (Barbaro et al, 2017; Reus‐Chavarria et al, 2019), and vasculature (Liu et al, 2015; Wang et al, 2018) can contribute to hypertension via mechanisms beyond the kidney. In addition, studies demonstrated that endothelial ENaC, termed EnNaC by David Warnock to distinguish it from epithelial ENaC (Warnock, 2013), highly resembled ENaC in the epithelia, which can also be regulated by the mineralocorticoid hormone aldosterone (Kusche‐Vihrog et al, 2008, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…However, during the past two decades, the renal ENaC mechanism responsible for hypertension has been expanded. Accumulating evidence has demonstrated that ENaC was widely expressed in nonepithelial tissues, including baroreceptors (Drummond et al, 1998(Drummond et al, , 2001, immune cells (Barbaro et al, 2017;Reus-Chavarria et al, 2019), neurons (Brockway et al, 2002), vascular endothelial cells (ECs) (Golestaneh et al, 2001;Kusche-Vihrog et al, 2010;Liu et al, 2015;Wang et al, 2009Wang et al, , 2018, and vascular smooth muscle cells (VSMCs) (Grifoni et al, 2006;Jernigan & Drummond, 2006;Jernigan et al, 2008), and so forth. This widespread expression profile of ENaC suggests its potential role under physiological and pathological conditions.…”

Section: Introductionmentioning

confidence: 99%

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Detrimental or beneficial: Role of endothelial ENaC in vascular function

Zhang

Yuan

Chen

et al. 2021

Journal Cellular Physiology

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In the past, it was believed that the expression of the epithelial sodium channel (ENaC) was restricted to epithelial tissues, such as the distal nephron, airway, sweat glands, and colon, where it is critical for sodium homeostasis. Over the past two decades, this paradigm has shifted due to the finding that ENaC is also expressed in various nonepithelial tissues, notably in vascular endothelial cells. In this review, the recent findings of the expression, regulation, and function of the endothelial ENaC (EnNaC) are discussed.The expression of EnNaC subunits is reported in a variety of endothelial cell lines and vasculatures, but this is controversial across different species and vessels and is not a universal finding in all vascular beds. The expression density of EnNaC is very faint compared to ENaC in the epithelium. To date, little is known about the regulatory mechanism of EnNaC. Through it can be regulated by aldosterone, the detailed downstream signaling remains elusive. EnNaC responds to increased extracellular sodium with the feedforward activation mechanism, which is quite different from the Na + self-inhibition mechanism of ENaC. Functionally, EnNaC was shown to be a determinant of cellular mechanics and vascular tone as it can sense shear stress, and its activation or insertion into plasma membrane causes endothelial stiffness and reduced nitric oxide production. However, in some blood vessels, EnNaC is essential for maintaining the integrity of endothelial barrier function. In this context, we discuss the possible reasons for the distinct role of EnNaC in vasculatures.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detrimental or beneficial: Role of endothelial ENaC in vascular function

Zhang

Yuan

Chen

et al. 2021

Journal Cellular Physiology

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“…A study by Jia et al found that EnaC activation mediates endothelial permeability, which in turn promotes macrophage infiltration, M1 polarization, and oxidative stress leading to cardiac fibrosis and diastolic dysfunction in female mice with dietary obesity ( 44 ). In human studies, there is increased expression of the EnaC in neutrophils from hypertensive patients suggesting an inflammatory role ( 123 ). Upregulation of the EnaC has been linked to stiffening of the cortical actin cytoskeleton in endothelial cells including those in the coronary vessels, impaired endothelial NO release, increased oxidative stress, increased vascular permeability, and immune activation ( 124 ).…”

Section: Epithelial Sodium Channel and Cardiovascular Diseasementioning

confidence: 99%

Hypertension and human immunodeficiency virus: A paradigm for epithelial sodium channels?

Mutengo

Masenga

Mwesigwa

et al. 2022

Front. Cardiovasc. Med.

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Hypertension is a risk factor for end organ damage and death and is more common in persons with HIV compared to the general population. Several mechanisms have been studied in the pathogenesis of hypertension. Current evidence suggests that the epithelial sodium channel (ENaC) plays a key role in regulating blood pressure through the transport of sodium and water across membranes in the kidney tubules, resulting in retention of sodium and water and an altered fluid balance. However, there is scarcity of information that elucidates the role of ENaC in HIV as it relates to increasing the risk for development or pathogenesis of hypertension. This review summarized the evidence to date implicating a potential role for altered ENaC activity in contributing to hypertension in patients with HIV.

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“…The ENaC controls the rate limiting step of sodium reabsorption in epithelial cells and is located on the apical membrane of cells in the distal nephron which is responsible for controlling Na + reabsorption. ENaC protein in urine is a biomarker of hypertension as ENaC expression promotes Na + retention, leading to an increase in blood pressure [30,31].…”

Section: Introductionmentioning

confidence: 99%

“…Typically, ENaC protein is detected by enzyme-linked immunosorbent assay (ELISA) [4,32], but this method is time consuming and expensive [27]. Immunosensors can detect low levels of ENaC [3,31] since using antibodies as bioreceptors is highly selective and sensitive [33]. However, antibody based methods are relatively expensive for commercial purposes and therefore alternative methods have been developed, such as the determination of ENaC protein levels through its interaction with aptamers [34].…”

Section: Introductionmentioning

confidence: 99%

Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application

Zakiyyah

Eddy

Firdaus

et al. 2022

J. Electrochem. Sci. Eng.

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A nanocomposite of natural silica and ceria was synthesized to modify a screen-printed carbon electrode (SPCE) to develop an aptasensor to detect epithelial sodium channel (ENaC) protein in urine as a biomarker of hypertension. The method steps were the synthesis of natural silica-ceria nanocomposite using the hydrothermal method, obtaining of natural silica nanoparticles from the extraction of alkaline silica sand and ceria nanoparticles from cerium nitrate, modification of SPCE/natural silica-ceria, immobilization of aptamer through streptavidin-biotin, and detection of ENaC protein concentration. Box-Behnken’s design was employed to determine the optimal conditions of aptamer concentration (0.5 μg mL-1), streptavidin incubation time (30 min), and aptamer incubation time (1 hour), respectively. Differential pulse voltammetry (DPV) characterization of the developed electrochemical aptasensor revealed that the [Fe(CN)6]3-/4- redox peak current increased from 3.190 to 9.073 μA, with detection and quantification limits of 0.113 and 0.343 ng mL-1, respectively. The method is proven as a simple and rapid method to monitor ENaC levels in urine samples.

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Enhanced expression of the Epithelial Sodium Channel in neutrophils from hypertensive patients

Cited by 20 publications

References 61 publications

Detrimental or beneficial: Role of endothelial ENaC in vascular function

Detrimental or beneficial: Role of endothelial ENaC in vascular function

Hypertension and human immunodeficiency virus: A paradigm for epithelial sodium channels?

Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application

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