Enhanced Freezability of Goat Spermatozoa Collected into Tubes Containing Extender Supplemented with Bovine Serum Albumin (BSA)

Yamashiro, Hideaki; Haifeng, Wang; Yamashita, Yugo; Kumamoto, Kanako; Takato, Tsuyoshi

doi:10.1262/jrd.17105

Cited by 15 publications

(8 citation statements)

References 23 publications

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“…In cattle, BSA is found in reproductive tract fluids, and helps to preserve sperm motility and acrosome integrity during the semen freezing process (Yamashiro et al 2006). In the present study, GSH and GGSG had a slight promoting effect on sperm motility, while ROS increased.…”

Section: Discussionsupporting

confidence: 49%

Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen

Sarıözkan¹,

Tuncer²,

Bucak³

et al. 2009

Acta Vet. Brno

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Cryopreservation is associated with the production of reactive oxygen substances (ROS), which lead to lipid peroxidation of sperm membranes, resulting in a loss of sperm motility, viability and fertility. The aim of this study was to determine effects of the antioxidants of oxidized glutathione (GSSG), reduced glutathione (GSH) and bovine serum albumin (BSA) on standard semen indicators (motility, acrosome and total abnormalities, HOST), endogenous antioxidant enzyme activities and fertilizing ability of frozen-thawed bull semen.Eighteen ejaculates from each of 3 Holstein bulls were collected using an artificial vagina and 9 replicates of the ejaculates were diluted with a Bioxcell ® -based extender supplemented with antioxidants, including BSA (5 mg/ml), GSH (2 mM), GGSG (2 mM), and an extender containing no antioxidants (control). Insemination doses (1.5 × 10 7 sperm/0.25 ml straw) were prepared for the insemination of cows at observed oestrus. Supplementation with antioxidants led to lower percentages of acrosome damage (4.0 ± 0.5%, 4.4 ± 0.5%, 4.0 ± 0.3%, respectively) and total abnormalities (10.3 ± 0.7%, 9.7 ± 0.8%, 10.4 ± 0.6%), compared to the controls (6.5 ± 0.6 and 14.9 ± 1.1% P < 0.01). Pregnancy rate after insemination was highest (72.2%) in the group which was given BSA (P < 0.05). There were no significant differences among groups in GSH and glutathione peroxidase (GSH-PX) enzyme activities. Superoxide dismutase (SOD) activities (0.05 ± 0.005, 0.03 ± 0.005, 0.05 ± 0.009 μkat/g protein, respectively) in all of the experimental groups with antioxidants were lower than the control group (0.11 ± 0.024 μkat/g protein, P < 0.001). Furthermore, BSA increased (P < 0.001) the activity of catalase (CAT, 304.23 ± 114.69 μkat/g protein), following the freezing-thawing process. Bull semen, freezing, artificial insemination, antioxidant activities, lipid peroxidationSemen cryopreservation has allowed specific opportunities for the conservation and widespread dissemination of valuable genetic resources through sperm banks, the guarantee of a constant commercial supply of semen, and collaboration in breed improvement programs by means of artificial insemination (AI) (Holt et al. 1997). Some of the problems encountered using AI in pigs, sheep and exotic species include decreased motility, membrane integrity and fertilizing potential of spermatozoa during cryopreservation, premature activation in the female genital tract, and altered membrane responses to physiological stimuli of sperm that survive the freezing-thawing process (Viswanath and Shannon 2000). It is well known that phospholipids in the sperm plasma membrane undergo peroxidation, which results in the formation of reactive oxygen substances (ROS, namely, superoxide anion radical, hydrogen peroxide) and lipid hydroperoxides (Alvarez and Storey 2005). Excessive generation of ROS by immature and abnormal spermatozoa, and sperm processing (e.g., extending, freezingthawing process), accompanied by low scavenging and antioxidant concentrations in semina...

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Section: Discussionsupporting

confidence: 49%

Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen

Sarıözkan¹,

Tuncer²,

Bucak³

et al. 2009

Acta Vet. Brno

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“…These studies showed that BSA significantly improved semen characteristics. BSA is found in reproductive tract fluids, and helps to preserve sperm motility and acrosomal integrity during the semen freezing process in goat [64]. Embryos from swine can be developed in vitro from the zygote to the blastocyst stage in a culture medium.…”

Section: Discussionmentioning

confidence: 99%

The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen

et al. 2013

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The aim of the present study was to determine the effects of the bovine serum albumin (BSA) and fetal calf serum (FCS) on sperm quality, DNA fragmentation and lipid peroxidation of liquid stored rabbit semen stored up to 72 h at 5 °C. Ejaculates were collected from five New Zealand male rabbits by artificial vagina and pooled at 37 °C following evaluation. Each pooled ejaculate was split into three equal experimental groups and diluted to a final concentration of approximately 40 × 10(6)sperm/ml (single step dilution), in an Eppendorf tube, with the Tris based extender containing BSA (5mg/ml), FCS (10%) or no additive (control) at 37 °C, cooled to 5 °C and stored for up to 72 h. The extender supplemented with BSA and FCS did not improve the percentages of motility and acrosomal abnormality during 48 h compared to the control. The additives BSA and FCS had a significant effect in the maintaining of plasma membrane integrity between 48 and 72 h storage period, compared to the control (P<0.01). The supplementation of BSA and FCS had a protective effect on motility (P<0.05), plasma membrane integrity (P<0.01) and acrosomal integrity (P<0.01) at 72 h compared to the control. The supplementations with BSA and FCS led to a reduction in DNA damage of rabbit sperm at 48 and 72 h during storage period, compared to the control (P<0.001). Although supplementation of BSA and FCS caused significant (P<0.01) decreases in malondialdehyde (MDA) level at 48 h and 72 h, they significantly (P<0.01) increased the glutathione peroxidase (GPx) antioxidant activity up to 72 h when compared to the control group. In conclusion, BSA and FCS supplementation to liquid stored rabbit semen provide a protection for spermatozoa against cool storage-induced DNA damage and plasma membrane integrity by their antioxidative properties.

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“…Kandungan BSA dalam CEP-2 merupakan makromolekul yang berperan mengikat ion Ca 2+ , mencegah masuknya ion Ca 2+ berlebihan ke sitosol, memungkinkan membran untuk lebih efektif dalam mengatur pergerakan ion Ca 2+ melewati membran dan menghambat akumulasi ion Ca 2+ intraseluler ke tingkat toksik bagi spermatozoa, sehingga viabilitas, motilitas dan jumlah spermatozoa belum terkapasitasi dapat dipertahankan tetap tinggi (Landim-Alvarenga et al, 2004). Yamashiro et al (2006) menjelaskan bahwa penambahan 5% BSA dalam pengencer Tris (hydroxymethyl-aminomethane)-citric acidglucose pada spermatozoakambing Shiba post-thawingmenghasilkan motilitas yang lebih tinggi (77,3+3,7)% dibandingkan dengan spermatozoa tanpa BSA (58,6+5,9)%. Penelitian Osman, et al (2012) melaporkan bahwa viabilitas spermatozoa sapi Piedmontese sebesar (38,743±0,854) % setelah penyimpanan pada suhu 4 o C selama 31 hari dengan level BSA 8 mg/ml.…”

Section: Pendahuluanunclassified

Aplikasi Inseminasi Buatan pada Induk Sapi Potong Menggunakan Se-men Cair Sapi Peranakan Ongole dengan Pengencer Cauda Epidydymal Plasma-2 + 0,6% Bovine Serum Albumin

Rachmawati

Ismaya²,

Widyobroto³

et al. 2018

JIIP

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Artificial insemination (AI) in cattle using Ongole Cross Breed liquid semen with cauda epidydymal plasma-2 (CEP-2) diluent+0.6% Bovine Serum Albumin (BSA) aimed to improve the genetic quality of livestock with high sperm motility on beef cows. Addition of BSA as extracellular cryoprotectant to support egg yolk function to prevent membrane damage caused by cold shock during cold storage. This research was conducted in Tumpang, Pakis, and Singosari Sub-district, Malang Regency using 38 cows that inseminated using frozen semen of Limousine and Simmental breeds as control (19 cows) and liquid semen with CEP-2 + 0.6% BSA as a treatment (19 cows). The reproductive parameters were Non Return Rate (NRR), Service per Conception (S/C) and Conception Rate (CR). Data were analyzed descriptively and continued by chi-square test. The results showed that insemination using frozen and liquid semen did not affect the success rate of AI in beef cows. The NRR43-63 value of frozen was 89.40% and liquid semen was 78.94%; the CR value of frozen semen was 84.21% and liquid semen was 57.89%; and the S/C value of frozen semen was 1.21 and liquid semen was 1.42. The conclusions of the study were the values of NRR43-63, CR and S/C of frozen higher than liquid semen.

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Enhanced Freezability of Goat Spermatozoa Collected into Tubes Containing Extender Supplemented with Bovine Serum Albumin (BSA)

Cited by 15 publications

References 23 publications

Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen

Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen

The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen

Aplikasi Inseminasi Buatan pada Induk Sapi Potong Menggunakan Se-men Cair Sapi Peranakan Ongole dengan Pengencer Cauda Epidydymal Plasma-2 + 0,6% Bovine Serum Albumin

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