Enhanced gene delivery to human airway epithelial cells using an integrin-targeting lipoplex

Scott, Emily S.; Wiseman, John; Evans, Martin; Colledge, William H

doi:10.1002/jgm.172

Cited by 38 publications

(23 citation statements)

References 36 publications

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“…We have previously shown that a peptide containing an integrin-binding RGD motif can enhance liposomemediated gene expression in 16HBE cells [28]. To determine if an integrin-binding domain and a NLS domain could enhance gene delivery more than each separate component we tested a bipartite VP1-RGD peptide.…”

Section: A Vp1-rgd Chimeric Peptide Does Not Enhance Liposome-mediatementioning

confidence: 99%

Enhancement of gene delivery to human airway epithelial cells in vitro using a peptide from the polyoma virus protein VP1

Wiseman

Scott

Shaw

et al. 2005

The Journal of Gene Medicine

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Section: A Vp1-rgd Chimeric Peptide Does Not Enhance Liposome-mediatementioning

confidence: 99%

Enhancement of gene delivery to human airway epithelial cells in vitro using a peptide from the polyoma virus protein VP1

Wiseman

Scott

Shaw

et al. 2005

The Journal of Gene Medicine

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“…Thus, this approach allows to discriminate between extracellularly bound and internalized complexes. 44,45 Indeed, the number of fluorescence positive cells decreased 20% after additional incubation of CellsScrub washing buffer (cellular internalization) compared to the number of fluorescence positive cells after washing with PBS (cellular association) alone. Similar observations were made for the mean fluorescence intensity with a decrease of 32%.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Tailor-Made Copolymers of Oligo(ethylene glycol) Methyl Ether Methacrylate and N,N-Dimethylaminoethyl Methacrylate as Nonviral Gene Transfer Agents: Influence of Macromolecular Structure on Gene Vector Particle Properties and Transfection Efficiency

et al. 2009

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Oligo(ethylene glycol) methyl ether methacrylates (OEGMA) of various chain lengths (i.e., 9, 23, or 45 EG units) and N,N-dimethylaminoethyl methacrylate (DMAEMA) were copolymerized by atom transfer radical polymerization (ATRP), yielding well-defined P(DMAEMA-co-OEGMA) copolymers with increasing OEGMA molar fractions (F OEGMA) but a comparable degree of polymerization (DP ∼ 120). Increase of both F OEGMA and OEGMA chain lengths correlated inversely with gene vector size, morphology, and zeta potential. P(DMAEMA-co-OEGMA) copolymers prevented gene vector aggregation at high plasmid DNA (pDNA) concentrations in isotonic solution and did not induce cytotoxicity even at high concentrations. Transfection efficiency of the most efficient P(DMAEMA-co-OEGMA) copolymers was found to be >10-fold lower compared with branched polyethylenimine (PEI) 25 kDa. Although OEGMA copolymerization largely reduced gene vector binding with the cell surface, cellular internalization of the bound complexes was less affected. These observations suggest that inefficient endolysosomal escape limits transfection efficiency of P(DMAEMA-co-OEGMA) copolymer gene vectors. Despite this observation, optimized p(DMAEMA-co-OEGMA) gene vectors remained stable under conditions for in vivo application leading to 7-fold greater gene expression in the lungs compared with PEI. Tailor-made P(DMAEMA-co-OEGMA) copolymers are promising nonviral gene transfer agents that fulfill the requirements for successful in vivo gene delivery.

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“…Several studies reported that EGFmodified lipid vesicles were constructed for effective delivery to tumor cells expressing high levels of the EGF receptor (Kikuchi et al 1996). A similar approach has been used for transferrin or lactoferrin with their receptors, and the RGD peptide with integrins (Cheng 1996;Elfinger et al 2007;Kunath et al 2003;Rao et al 2008;Scott et al 2001). In this regard, Siglecs also exhibited enhanced efficiency of uptake of binding substances.…”

Section: Discussionmentioning

confidence: 99%

Enhanced lentiviral vector production in 293FT cells expressing Siglec-9

et al. 2014

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Siglecs, sialic acid-recognizing Ig-superfamily lectins, regulate various aspects of immune responses, and have also been shown to induce the endocytosis of binding materials such as anti-Siglec antibodies or sialic acid-harboring bacteria. In this study, we demonstrated that the expression of Siglec-9 enhanced the transfection efficiency of several cell lines such as macrophage RAW264 and non-hematopoietic 293FT cells. We applied this finding to the production of a lentiviral vector in which cells were transfected simultaneously with multiple vectors, and achieved a twice increase in viral production levels. Furthermore, 293FT cells expressing lectin-defective Siglec-9 produced threeto seven-fold higher titer of viral vector compared with parental 293FT cells. These results suggest that Siglec-9 enhanced lentiviral vector production in a lectin-independent manner.

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Enhanced gene delivery to human airway epithelial cells using an integrin-targeting lipoplex

Abstract: These results indicate that integrin-binding peptides represent one way to enhance liposome-mediated gene delivery to pulmonary epithelia.

Cited by 38 publications

References 36 publications

Enhancement of gene delivery to human airway epithelial cells in vitro using a peptide from the polyoma virus protein VP1

Enhancement of gene delivery to human airway epithelial cells in vitro using a peptide from the polyoma virus protein VP1

Characterization of Tailor-Made Copolymers of Oligo(ethylene glycol) Methyl Ether Methacrylate and N,N-Dimethylaminoethyl Methacrylate as Nonviral Gene Transfer Agents: Influence of Macromolecular Structure on Gene Vector Particle Properties and Transfection Efficiency

Enhanced lentiviral vector production in 293FT cells expressing Siglec-9

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