Enhanced Glutamic Acid Production of Brevibacterium sp. with Temperature Shift-Up Cultivation

CHOI, SUN-UK; Nihira, Takuya; Yoshida, Toshiomi

doi:10.1263/jbb.98.211

Cited by 4 publications

(4 citation statements)

References 10 publications

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“…During the initial phase of growth, glucose was consumed at a specific rate (q glc ) of 1.53±0. (Choi et al, 2004). The most noticeable phenomenon observed was the overflow of organic acids during the growing phase without oxygen limitation and no heat or nutritional stress.…”

Section: Resultsmentioning

confidence: 99%

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

et al. 2009

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Corynebacterium glutamicum is a biotin-auxotrophic bacterium and some strains efficiently produce glutamic acid under biotin-limiting conditions. In an effort to understand C. glutamicum metabolism under biotin limitation, growth of the type strain ATCC 13032 was investigated in batch cultures and a time-course analysis was performed. A transient excretion of organic acids was observed and we focused our attention on lactate synthesis. Lactate synthesis was due to the ldh-encoded l-lactate dehydrogenase (Ldh). Features of Ldh activity and ldh transcription were analysed. The ldh gene was shown to be regulated at the transcriptional level by SugR, a pleiotropic transcriptional repressor also acting on most phosphotransferase system (PTS) genes. Electrophoretic mobility shift assays (EMSAs) and site-directed mutagenesis allowed the identification of the SugR-binding site. Effector studies using EMSAs and analysis of ldh expression in a ptsF mutant revealed fructose 1-phosphate as a highly efficient negative effector of SugR. Fructose 1,6-bisphosphate also affected SugR binding.

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Section: Resultsmentioning

confidence: 99%

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

et al. 2009

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“…Such phenomena can be explained by the effect of temperature on glutamic acid production. It is proven that temperature can influence the efflux of glutamic acid from bacteria cells in which the yield and the specific glutamic acid production rate can be increased by a temperature shift-up (Choi et al 2004). Increasing temperature is primarily responsible for a repression or inhibition of α-ketoglutarate dehydrogenase complex (Uy et al 2003).…”

Section: Effect Of Temperature On Glutamic Acid and Gaba Productionmentioning

confidence: 99%

Modeling of glutamic acid production by Lactobacillus plantarum MNZ

Zareian¹,

Ebrahimpour²,

Mohamed³

et al. 2013

Electron. J. Biotechnol.

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Background: L-glutamic acid, the principal excitatory neurotransmitter in the brain and an important intermediate in metabolism acts as a precursor of γ-amino butyric acid (GABA). In the present study, culture condition for enhanced glutamic acid production by Lactobacillus plantarum MNZ was optimized and the influence of such conditions on GABA production was evaluated. Results: Results indicated that glutamic acid increased up to 3-fold (3.35) under the following condition: pH 4.5, temperature 37ºC, 12% (w/v) glucose and 0.7% (w/v) ammonium nitrate; whilst GABA production was enhanced up to 10-fold under the following condition: pH 4.5, temperature 37ºC, 6% (w/v) glucose and 0.7% (w/v) ammonium nitrate. Conclusions: This is the first report for dual biosynthesizing activities of a lactic acid bacterium for the production of glutamic acid and GABA. The results of this study can be further used for developing functional foods rich inglutamic acid and subsequently GABA as a bioactive compound.

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“…Encouraging results were obtained for definite improvements over the existing ones in terms of carbon source, product purity, yield, concentration, and process intensification. In the literature, yields of GA of about 66.3 g g –1 (GA concentration 25 g L –1 ), and 48 g g –1 (GA concentration 41.4 g L –1 ) have been reported in batch mode fermentation, which are comparatively low.…”

Section: Discussionmentioning

confidence: 98%

Production of L (+) Glutamic Acid in a Fully Membrane-Integrated Hybrid Reactor System: Direct and Continuous Production under Non-Neutralizing Conditions

Vikramachakravarthi

Kumar

Pal

2014

Ind. Eng. Chem. Res.

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7Experimental investigations were carried out on continuous and direct production of L-8 glutamic acid in a hybrid reactor system that integrated conventional fermentative production 9 step with downstream membrane-based separation and purification in flat sheet cross flow 10 membrane modules. To ensure constant fermentor capacity, the system was operated at 11 steady state at different set flux values ranging from 76 L m -2 h -1 to 90 L m -2 h -1 at a dilution 12 rate of 0.15 h -1 . Overcoming the substrate-product inhibitions of traditional batch production 13 system, this new, compact, flexible and largely fouling-free design ensured steady and 14 continuous production of L-glutamic acid directly from a renewable carbon source at the rate 15 of about 8.4 g L -1 h -1 . Provisions of continuous product withdrawal, separation and recycling 16 of unconverted sugar and microbial cells ensured almost inhibition-free production under 17 high cell concentration. Well-screened nanofiltration membranes with high selectivity helped 18 achieve over 97% product purity while ensuring recovery and recycle of more than 95% 19 unconverted carbon source resulting in high yield of 0.95gg -1 . The direct production scheme 20 involves no phase change and use of harsh chemicals. The study presents development of a 21 green process of glutamic acid production for sustainable business.22 23

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Enhanced Glutamic Acid Production of Brevibacterium sp. with Temperature Shift-Up Cultivation

Cited by 4 publications

References 10 publications

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

Modeling of glutamic acid production by Lactobacillus plantarum MNZ

Production of L (+) Glutamic Acid in a Fully Membrane-Integrated Hybrid Reactor System: Direct and Continuous Production under Non-Neutralizing Conditions

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