Enhanced growth of neural networks on conductive cellulose-derived nanofibrous scaffolds

Kuzmenko, Volodymyr; Kalogeropoulos, T.; Thunberg, Johannes; Johannesson, Sara; Hägg, Daniel; Enoksson, Peter; Gatenholm, Paul

doi:10.1016/j.msec.2015.08.012

Cited by 55 publications

(28 citation statements)

References 44 publications

(48 reference statements)

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“…We have evaluated the influence of the conductive scaffolds on the neural cell differentiation. As shown in the previous study of Kuzmenko et al [14], the electrically conductive scaffolds that were 3D printed with NFC/CNT ink facilitate the growth and differentiation of SH-SY5Y cells. Our results have shown that the most conductive 20% CNTs scaffolds have no attached living cells, while there were many cells on the 10% CNT constructs.…”

Section: The Conductive Ink Promotes Differentiation Of Sh-sy5y Cellsmentioning

confidence: 63%

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3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells

Bordoni¹,

Karabulut

Kuzmenko

et al. 2020

Cells

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We prepared cellulose nanofibrils-based (CNF), alginate-based and single-walled carbon nanotubes (SWCNT)-based inks for freeform reversible embedding hydrogel (FRESH) 3D bioprinting of conductive scaffolds. The 3D printability of conductive inks was evaluated in terms of their rheological properties. The differentiation of human neuroblastoma cells (SH-SY5Y cell line) was visualized by the confocal microscopy and the scanning electron microscopy techniques. The expression of TUBB3 and Nestin genes was monitored by the RT-qPCR technique. We have demonstrated that the conductive guidelines promote the cell differentiation, regardless of using differentiation factors. It was also shown that the electrical conductivity of the 3D printed scaffolds could be tuned by calcium–induced crosslinking of alginate, and this plays a significant role on neural cell differentiation. Our work provides a protocol for the generation of a realistic in vitro 3D neural model and allows for a better understanding of the pathological mechanisms of neurodegenerative diseases.

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Section: The Conductive Ink Promotes Differentiation Of Sh-sy5y Cellsmentioning

confidence: 63%

“…Moreover, it should mimic the environment in which cells usually live. In 2016, Kuzmenko et al have prepared nanofibrillated cellulose-based conductive guidelines (NFC) functionalized with carbon nanotubes (CNTs) [14]. It has been demonstrated that the 3D-printed NFC scaffolds have a surface roughness that enhances attachment of SH-SY5Y cells.…”

Section: Of 11mentioning

confidence: 99%

3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells

Bordoni¹,

Karabulut

Kuzmenko

et al. 2020

Cells

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“…Integration of a nanomaterial with paper may lead to improved quality of the paper‐based devices such as enhanced separation, color contrast, and loading of the biomolecules, etc. The nanomaterial‐modified paper has been predicted to have applications in bioassays, drug screening, ELISA, cell culture studies, scaffolds, and biosensing applications.…”

Section: Application Of Nanomaterial‐modified Cpmentioning

confidence: 99%

Nanomaterial‐Modified Conducting Paper: Fabrication, Properties, and Emerging Biomedical Applications

et al. 2019

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The emerging demand for wearable, lightweight portable devices has led to the development of new materials for flexible electronics using non‐rigid substrates. In this context, nanomaterial‐modified conducting paper (CP) represents a new concept that utilizes paper as a functional part in various devices. Paper has drawn significant interest among the research community because it is ubiquitous, cheap, and environmentally friendly. This review provides information on the basic characteristics of paper and its functionalization with nanomaterials, methodology for device fabrication, and their various applications. It also highlights some of the exciting applications of CP in point‐of‐care diagnostics for biomedical applications. Furthermore, recent challenges and opportunities in paper‐based devices are summarized.

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“…3-D scaffolds are able to organize the stem cells into a higher ordered construct to achieve the optimal neural tissue ECM. Microspheres, [25] fibers, [26,27] (self-assembling peptides [28,29] or polymeric [30]) hydrogels, [31] and conductive scaffolds [32,33] (4) Col+FN+LN, in both hypoxia and normoxia conditions. The microfluidic chip contained 8 units in a single device.…”

Section: Role Of Microfluidic Devices In Neural Differentiationmentioning

confidence: 99%

Application of microfluidic systems for neural differentiation of cells

Hesari¹,

Mottaghitalab

Shafiee

et al. 2019

PRNANO

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Neural differentiation of stem cells is an important issue in the development of the central nervous system. Different methods such as chemical stimulation with small molecules, scaffolds, and microRNA can be used for inducing the differentiation of neural stem cells. However, microfluidic systems with the potential to induce neuronal differentiation have established their reputation in the field of regenerative medicine. Organization of the microfluidic system represents a novel model that mimics the physiologic microenvironment of cells among other two-and threedimensional cell culture systems. The microfluidic system has a patterned and well-organized structure that can be combined with other differentiation techniques to provide optimal conditions for neuronal differentiation of stem cells. In this review, different methods for effective differentiation of stem cells to neuronal cells are summarized. The efficacy of microfluidic systems in promoting neuronal differentiation is also addressed.

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Enhanced growth of neural networks on conductive cellulose-derived nanofibrous scaffolds

Cited by 55 publications

References 44 publications

3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells

3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells

Nanomaterial‐Modified Conducting Paper: Fabrication, Properties, and Emerging Biomedical Applications

Application of microfluidic systems for neural differentiation of cells

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