Enhanced healing and anti-inflammatory effects of a carbohydrate polymer with zinc oxide in patients with chronic venous leg ulcers: preliminary results

Moreno-Eutímio, Mario Adán; Espinosa-Monroy, Lorena; Orozco-Amaro, Tania; Torres-Ramos, Yessica Dorín; Montoya-Estrada, Araceli; Hicks, Juan José; Rodríguez-Ayala, Ernesto; Moral, Pamela Del; Moreno, José; Cueto-Garcia, Jorge

doi:10.5114/aoms.2016.59851

Cited by 12 publications

(14 citation statements)

References 28 publications

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“…Our study showed that amarogentin treatment attenuates the alterations in CTX, OC, BSAP, and PINP levels in the serum of OVR rats. Inflammatory cytokines play an important role in the regulation of bone resorption, but a deficiency of oestrogen results in their overexpression [17][18][19][20]. Our study revealed that amarogentin-treated rats exhibited a significant reduction in the levels of inflammatory cytokines (p < 0.01) compared to OVR rats.…”

Section: Discussionmentioning

confidence: 55%

Amarogentin promotes osteoblast differentiation in oestrogen-deficiency-induced osteoporosis rats by modulating the Nrf-2/MAPK/ERK signalling pathway

et al. 2019

Arch Med Sci

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Introduction: The beneficial effect of amarogentin in the management of osteoporosis was determined using in vivo and in vitro methods. Material and methods: Experimental osteoporosis was induced in rats via bilateral ovariectomy. Rats were then treated for 5 weeks with amarogentin (50 and 100 mg/kg, p.o.). The levels of several biochemical markers of bone resorption and formation as well as bone mineral density (BMD) were measured in the rat serum. Isolated rat bone tissues were analysed using western blot assays. In the in vitro study, MG63 human osteoblasts were treated with amarogentin (0-100 µg/ml), after which alkaline phosphatase activity and osteoblast proliferation were evaluated. Osteoblasts treated with amarogentin and inhibitors of extracellular signal-regulated kinase (ERK) were further examined via western blotting. Results: In the rat model of oestrogen-deficiency-induced osteoporosis, BMD was significantly enhanced (p < 0.01) and levels of inflammatory cytokines were reduced in amarogentin-treated animals vs. the controls. Amarogentin treatment also attenuated the altered levels of osteocalcin, C-telopeptide of type 1 collagen, procollagen type I N-terminal propeptide, and bone-specific alkaline phosphatase, and the altered expression of Akt, Nrf-2, ERK, and nuclear factor-κB p65 in the serum of rats with osteoporosis. In the in vitro study, amarogentin treatment enhanced alkaline phosphatase activity and osteoblast proliferation compared to the non-treated control. Amarogentin treatment alone enhanced the expression of pERK compared to treatment with an amarogentin + ERK inhibitor. Conclusions: Both the in vivo and the in vitro studies demonstrated the protective effect of amarogentin against oestrogen-deficiency-induced osteoporosis in rats. The mechanism seems to involve the amarogentin-mediated enhancement of osteoblast differentiation via the Nrf-2/MAPK/ERK signalling pathway.

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Section: Discussionmentioning

confidence: 55%

Amarogentin promotes osteoblast differentiation in oestrogen-deficiency-induced osteoporosis rats by modulating the Nrf-2/MAPK/ERK signalling pathway

et al. 2019

Arch Med Sci

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“…Although the majority of wounds seen in medical practice heal by primary intention, when they become chronic ulcers, bacterial entry and colonization are unavoidable [23]. Moreover, ongoing inflammation contributes to the colonization of chronic wounds by infectious agents [12]. Treatments for these ulcers include local products to prevent and control inflammation and microbial growth, preferably avoiding antibiotics.…”

Section: Discussionmentioning

confidence: 99%

“…As already mentioned, it is now preferred that treatments are free of antibiotics to reduce the risk of resistance. The definite anti-biofilm activity of CPZO adds to its known anti-inflammatory and pro-healing properties [8][9][10][11][12][13][14][15].…”

Section: Discussionmentioning

confidence: 99%

“…Pebisut is the commercial name in Mexico of a biodegradable adhesive carbohydrate polymer with ZnO (CPZO), which enhances healing and possesses anti-inflammatory activity in patients with CVUs [8]. Although preliminary clinical data suggest that this adhesive mixture decreases infection rates in CVUs, its bactericidal activity remains unknown to date [9][10][11][12][13][14][15]. Therefore, we examined the bactericidal activity of CPZO against Gram-positive and Gram-negative bacteria, all of which frequently complicate chronic wounds.…”

Section: Introductionmentioning

confidence: 99%

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In vitro bactericidal activity of a carbohydrate polymer with zinc oxide for the treatment of chronic wounds

Bello–López

López-Ornelas

Vilchis-Rangel

et al. 2020

Journal of Medical Microbiology

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Introduction. Biological adhesives and effective topical therapeutic agents that improve wound healing are urgently required for the treatment of chronic ulcers. A biodegradable adhesive based on a carbohydrate polymer with zinc oxide (CPZO) was shown to possess anti-inflammatory activity and enhance wound healing, but its bactericidal activity was unknown. Aim. To investigate the bactericidal activity of CPZO against bacteria commonly present as infectious agents in chronic wounds. Methodology. We examined the bactericidal activity of CPZO against three biofilm-producing bacteria ( Staphylococcus aureus , Escherichia coli and Pseudomonas aeruginosa ) through three strategies: bacterial suspension, biofilm disruption and in vitro wound biofilm model. Results. In suspension cultures, CPZO had direct, potent bactericidal action against S. aureus within 24 h, whereas E. coli took 7 days to be eliminated. By contrast, P. aeruginosa survived up to 14 days with CPZO. CPZO had biofilm disruption activity against clinical isolates of S. aureus in the anti-biofilm test. Finally, in the in vitro wound biofilm model, CPZO dramatically reduced the bacterial viability of S. aureus and P. aeruginosa . Conclusions. Together with its previously shown anti-inflammatory properties, the bactericidal activity of CPZO gives it the potential to be a first-line therapeutic option for chronic various ulcers and, possibly, other chronic ulcers, preventing or controlling microbial infections, and leading to the healing of such complicated chronic ulcers.

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“…The varicose veins of the lower extremities are the most common peripheral vascular disease. About 23% of adults suffer from varicose veins of the lower extremities, and the current number has exceeded 25 million [9], [10]. In China, the prevalence of varicose veins has exceeded 8% [11].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Varicose Veins of Lower Extremities Based on Vascular Endothelial Cell Inflammation Images and Multi-Scale Deep Learning

et al. 2019

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The doctor determines whether there are lesions in the human body through the diagnosis of medical images, and classifies and identifies the lesions. Therefore, the automatic classification and recognition of medical images has received extensive attention. Since the inflammatory phenomenon of vascular endothelial cells is closely related to the varicose veins of the lower extremities, in order to realize the automatic classification and recognition of varicose veins of the lower extremities, this paper proposes a varicose vein recognition algorithm based on vascular endothelial cell inflammation images and multi-scale deep learning, called MSDCNN. First, we obtained images of vascular endothelial cells in patients with varicose veins of the lower extremities and normal subjects. Second, multiple convolutional layers extract multi-scale features of vascular endothelial cell images. Then, the MFM activation function is used instead of the ReLU activation function to introduce a competitive mechanism that extracts more features that are compact and reduces network layer parameters. Finally, the network uses a 3 × 3 convolution kernel to improve the network feature extraction capability and use the 1 × 1 convolution kernel for dimensionality reduction to further streamline network parameters. The experimental results tell us that the network has the advantages of high recognition accuracy, fast running speed, few network parameters, and is suitable for small-embedded devices. INDEX TERMS Vascular endothelial cells, inflammation, multi-scale deep learning, varicose veins of the lower extremities.

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Enhanced healing and anti-inflammatory effects of a carbohydrate polymer with zinc oxide in patients with chronic venous leg ulcers: preliminary results

Cited by 12 publications

References 28 publications

Amarogentin promotes osteoblast differentiation in oestrogen-deficiency-induced osteoporosis rats by modulating the Nrf-2/MAPK/ERK signalling pathway

Amarogentin promotes osteoblast differentiation in oestrogen-deficiency-induced osteoporosis rats by modulating the Nrf-2/MAPK/ERK signalling pathway

In vitro bactericidal activity of a carbohydrate polymer with zinc oxide for the treatment of chronic wounds

Analysis of Varicose Veins of Lower Extremities Based on Vascular Endothelial Cell Inflammation Images and Multi-Scale Deep Learning

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