Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment

Abstract

The high molecular weight and poor solubility of seaweed polysaccharides have limited their function and application. In this study, ultraviolet/hydrogen peroxide (UV/H2O2) treatment was used to prepare low-molecular-weight seaweed polysaccharides from Sargassum fusiforme. The effects of UV/H2O2 treatment on the physicochemical properties and anti-photoaging activity of S. fusiforme polysaccharides were studied. UV/H2O2 treatment effectively degraded polysaccharides from S. fusiforme (DSFPs), reducing their molecular weight from 271 kDa to 26 kDa after 2 h treatment. The treatment did not affect the functional groups in DSFPs but changed their molar percentage of monosaccharide composition and morphology. The effects of the treatment on the anti-photoaging function of S. fusiforme polysaccharides were investigated using human epidermal HaCaT cells in vitro. DFSPs significantly improved the cell viability and hydroxyproline secretion of UVB-irradiated HaCaT cells. In particular, DSFP-45 obtained from UV/H2O2 treatment for 45 min showed the best anti-photoaging effect. Moreover, DSFP-45 significantly increased the content and expression of collagen I and decreased those of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α. Thus, UV/H2O2 treatment could effectively improve the anti-photoaging activity of S. fusiforme polysaccharides. These results provide some insights for developing novel and efficient anti-photoaging drugs or functional foods from seaweed polysaccharides.

Anti-Photoaging Effects of Upcycled Citrus junos Seed Anionic Peptides on Ultraviolet-Radiation-Induced Skin Aging in a Reconstructed Skin Model

“…UVB exposure induces oxidative stress in keratinocytes [19], and increased production of ROS is deleterious to keratinocytes [19]. However, previously, peptides from seaweed [20], a frog peptide [16], and a porcine placenta peptide [17] were reported to prevent the photoaging of skin by inhibiting ROS accumulation. Also, a peptide from tilapia gelatin hydrolysate inhibited ROS production by enhancing the levels of antioxidant factors superoxide (SOD) and glutathione (GSH) in UVB-irradiated HaCaT cells [18].…”

Effects of UV/H2O2 Degradation on the Physicochemical and Antibacterial Properties of Fucoidan

“…Our research group previously found that ultraviolet/hydrogen peroxide (UV/H 2 O 2 ) treatment could be used to degrade marine polysaccharides. For example, it could effectively reduce the molecular weight of Sargassum fusiforme polysaccharide and enhance its anti-inflammatory and anti-photoaging activities [10,11]. Whether the UV/H 2 O 2 treatment could degrade fucoidan and improve its activity needs to be explored.…”