Enhanced incorporation of nonhydrolyzable tritium in GnRH and TRF by catalytic exchange labeling

Abstract

SummarY Gonadotropin releasing hormone (GnRH),

Direct metal‐catalyzed tritiation of organic compounds

“…The heterogeneous catalyst system that we have had the most experience and success with for tritiations has been 5% rhodium on alumina. Others have previously reported the use of this catalyst for hydrogen exchange chemistry,2–5 including the most recent and intriguing paper by Jones and Lockley 6. No doubt, the support structure and its chemistry are important to this catalyst's performance and earlier mechanistic studies with 5% rhodium on alumina have also examined the possible participation of the surface hydroxyl groups in the hydrogen exchange process 2.…”

Synthesis of [2,6-3H2-Tyr1]leucine-enkephalin

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The synthesis of [2, leucine-enkephalin by the catalytic tritiation of [2,6-dibromo-Tyr'] leucine-enkephalin is described. The precursor amino acid, 2,6-dibromo-~~-tyrosine, was synthesized in three steps from 2,6-di bromo-4-methoxytoluene.

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