Enhanced inulinase production by Streptomyces sp. in solid state fermentation through statistical designs

Abstract

In this work, inulinase was produced by solid state fermentation by Streptomyces sp. using copra waste as carbon source. The nutrients were screened by Plackett–Burman design. From the pareto chart it was found that the nutrients, namely, soya bean cake, MgSO4·7H2O and (NH4)2SO4 were found to be most significant nutrient components. Hence, these three components were selected for further optimization using central composite design (CCD) in response surface methodology (RSM). The optimum conditions were soya bean cake: 0.05711 g/gds, MgSO4·7H2O: 0.00063 g/gds and (NH4)2SO4: 0.00772 g/gds. Under these optimized conditions, the production of inulinase was found to be 131 U/gds. The constants in the Michaelis–Menten equation were evaluated and high R2 value implies the fitness of the model.

Recombinant expression, characterization and application of a dihydrolipoamide dehydrogenase with diaphorase activity from Bacillus sphaericus

“…They have the potential to be used as a component of in vitro diagnostic tests, e.g., diagnostic assays for PKU (Dilipkumar et al 2013), MSUD and galactosemia (Shahbazmohammadi and Omidinia 2011). A diagnostic test in which the potential of desired enzyme was examined can be exemplified in the determination of galactose.…”

“…These enzymes have potential application to be used as a component of assay systems for the determination of different substances such as leucine, phenylalanine and ethanol. They have been used in biotransfomation reactions (Bhushan et al 2002), biosensor design (Antiochia et al 1997) and in vitro diagnostic tests such as phenylketonuria (PKU) (Dilipkumar et al 2013;Shahbazmohammadi and Omidinia 2011), maple syrup urine diseases (MSUD) and galactosemia as well (Kianmehr et al 2016). Due to industrial applications of diaphorases, these enzymes have attracted researcher's attentions for screening of novel sources and cost-benefit production.…”

Bio-prospecting of cuttle fish waste and cow dung for the production of fibrinolytic enzyme from Bacillus cereus IND5 in solid state fermentation

“…Agro-industrial wastes, waste water and fishery wastes were utilized as cost-effective substrates in processes of biomolecule production. The agro-wastes namely, green gram husk (Prakasham et al 2006), cake of Jatropha curcas seed (Mahanta et al 2008), ground nut husk (Salihu et al 2014), copra waste (Dilipkumar et al 2013) and wheat bran and deproteinized acid cheese whey (Raol et al 2015) were used successfully as the substrate for the enzyme production. The ideal substrate should be cheap so as to reduce the production cost of biomolecules (Pandey et al 2000).…”

Optimisation of alpha-amylase inhibitor production in solid state fermentation

“…to secrete extracellular enzymes such as hydrolases which are used to utilise both simple and complex molecules as nutrients makes them able to effectively utilise wheat bran as a solid substrate (Kapur et al, 2018). Wheat bran and the basal medium also contain nitrogen sources which have been found to have significant effects in the secondary metabolism of Streptomyces (Dilipkumar et al, 2013). In fact, cornsteep liquor and soybean flour were identified as Nitrogen sources which are necessary in the synthesis of inhibitor by Streptomyces (Vertesy et al, 1984).…”