Enhanced Lycopene Production in Escherichia coli by Expression of Two MEP Pathway Enzymes from Vibrio sp. Dhg

Kim, Min Jae; Noh, Myung Hyun; Woo, Sunghwa; Lim, Hyun Gyu; Jung, Gyoo Yeol

doi:10.3390/catal9121003

Cited by 14 publications

(13 citation statements)

References 50 publications

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“…In addition, Lycopene is a carotenoid referring to C40 terpenoids and is broadly found in various plants, particularly vegetables and fruits. It has been shown that MVA and MEP-pathways directly influence the biosynthesis production of Lycopene (Kim et al, 2019; Wei et al, 2018). While Wille et al (2004) showed that β-carotene and Lutein are synthesized using intermediates from the MEP pathway, Opitz et al (2014) revealed that both MVA and MPE pathways contribute to producing isoprenoids such as β-carotene and Lutein.…”

Section: Resultsmentioning

confidence: 99%

Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways

Movahedi

Wei

Pucker

et al. 2020

Preprint

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Plants use two distinct isoprenoid biosynthesis pathways: the methylerythritol phosphate (MEP) pathway and mevalonic acid (MVA) pathway. 1-deoxy-D-xylulose5-phosphate synthase (DXS) and 1-deoxy-D-xylulose5-phosphate reductoisomerase (DXR) are the rate-limiting enzymes in the MEP pathway, and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a key regulatory enzyme in the MVA pathway. Previously, overexpression of Populus trichocarpa PtDXR in Nanlin 895 poplar was found to upregulate resistance to salt and drought stresses, and the transgenic poplars showed improved growth. In the present study, PtHMGR overexpressors (OEs) exhibited higher expression levels of DXS, DXR, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase (HDS), and 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) and lower expression levels of 2-C-methyl-d-erythritol4-phosphate cytidylyltransferase (MCT), 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK), and 3-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGS) than non-transgenic poplars (NT). However, the poplar PtDXR-OEs showed upregulated expression levels of MEP-related genes and downregulated expression of MVA-related genes. Moreover, overexpression of PtDXR and PtHMGR in poplars caused changes in MVA-derived trans-zeatin-riboside (TZR), isopentenyl adenosine (IPA), castasterone (CS) and 6-deoxocastasterone (DCS), as well as MEP-derived carotenoids, gibberellins (GAs), and abscisic acid (ABA). In PtHMGR-OEs, greater accumulation of geranyl diphosphate synthase (GPS) and geranyl pyrophosphate synthase (GPPS) transcript levels in the MEP pathway led to accumulation of MEP-derived isoprenoids, while upregulation of farnesyl diphosphate synthase (FPS) expression in the MVA pathway contributed to increased levels of MVA-derived isoprenoids. Similarly, in PtDXR-OEs, increased GPS and GPPS transcript levels in the MEP pathway boosted MEP-derived isoprenoid levels and changes in FPS expression affected MVA-derived isoprenoid yields. From these results, we can conclude that cross-talk exists between the MVA and MEP pathways.

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Section: Resultsmentioning

confidence: 99%

Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways

Movahedi

Wei

Pucker

et al. 2020

Preprint

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“…SP1, three heterologous genes ( crtE encoding geranylgeranyl diphosphate (GGPP) synthase, crtB encoding phytoene synthase and crtI encoding phytoene desaturase) from Lamprocystis purpurea were introduced for lycopene production, and additional crtY gene encoding lycopene cyclase from Pantoea ananatis was introduced for β -carotene production. For the plasmid of lycopene production, p1EBI plasmid which encodes crtE-crtB-crtI gene under same P tac promoter in a polycistronic manner was used [23] . To prepare β -carotene plasmid, p1EBIY, vector fragment was amplified using the primers of crtY_v-F and crtY_v-R with p1EBI as a template.…”

Section: Methodsmentioning

confidence: 99%

“…dhg [22] was characterized to utilize alginate and produce diverse high-value added products, while exhibiting fast growing profile and engineering potential. Further investigation revealed that enzymes mediating the methylerythritol-4-phosphate (MEP) pathway found in the strain exhibits better activity than those of E. coli , implying Vibrio species could be an promising workhorse when producing isoprenoid or carotenoid metabolites [23] . Likewise, the accumulation of microbial and genetic resources is expected to enrich the realm of genetic compartments regarding metabolic engineering of Vibrio species for the production of value-added products.…”

Section: Introductionmentioning

confidence: 99%

Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae

Park

Cho

Lee

et al. 2021

Computational and Structural Biotechnology Journal

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“…Many challenges in retinal production have been addressed using metabolically engineered E. coli cells. Several aspects have been considered for the efficient production of carotenoid, which can be used as a substrate for BCOs, from glucose or glycerol via the endogenous MEP pathway and exogenous MVA pathway in metabolically engineered cells [63][64][65][66][67][68]. However, studies on the engineering of BCOs, which are crucial enzymes for catalyzing the last step of the retinal biosynthetic pathway, are still lacking.…”

Section: Future Perspectives Of Bco In Synthetic Bacteriamentioning

confidence: 99%

Molecular Properties of β-Carotene Oxygenases and Their Potential in Industrial Production of Vitamin A and Its Derivatives

et al. 2022

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β-Carotene 15,15′-oxygenase (BCO1) and β-carotene 9′,10′-oxygenase (BCO2) are potential producers of vitamin A derivatives, since they can catalyze the oxidative cleavage of dietary provitamin A carotenoids to retinoids and derivative such as apocarotenal. Retinoids are a class of chemical compounds that are vitamers of vitamin A or are chemically related to it, and are essential nutrients for humans and highly valuable in the food and cosmetics industries. β-carotene oxygenases (BCOs) from various organisms have been overexpressed in heterogeneous bacteria, such as Escherichia coli, and their biochemical properties have been studied. For the industrial production of retinal, there is a need for increased production of a retinal producer and biosynthesis of retinal using biocatalyst systems improved by enzyme engineering. The current review aims to discuss BCOs from animal, plants, and bacteria, and to elaborate on the recent progress in our understanding of their functions, biochemical properties, substrate specificity, and enzyme activities with respect to the production of retinoids in whole-cell conditions. Moreover, we specifically propose ways to integrate BCOs into retinal biosynthetic bacterial systems to improve the performance of retinal production.

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Enhanced Lycopene Production in Escherichia coli by Expression of Two MEP Pathway Enzymes from Vibrio sp. Dhg

Cited by 14 publications

References 50 publications

Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways

Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways

Engineering Vibrio sp. SP1 for the production of carotenoids directly from brown macroalgae

Molecular Properties of β-Carotene Oxygenases and Their Potential in Industrial Production of Vitamin A and Its Derivatives

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