Enhanced lysophosphatidylcholine and sphingomyelin contents are characteristic of spermatozoa from obese men—A MALDI mass spectrometric study

Pyttel, Susanne; Zschörnig, Kristin; Nimptsch, Ariane; Paasch, Uwe; Schiller, Jürgen

doi:10.1016/j.chemphyslip.2012.11.001

Cited by 21 publications

(17 citation statements)

References 38 publications

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“…Lipid attributions were based on a literature and database search. Their presence in human sperm was observed in previous reports by MALDI-MS (16,17,30).…”

Section: Lipid Profile Of Sperm Membranessupporting

confidence: 70%

“…To better visualize the differences introduced by the soy-PC treatment, we performed PCA of the pairs TEST versus TEST-PC and TEST-Y versus TEST-Y-PC. The ions that contributed to statistical discriminations were assigned by a literature search for previous studies reporting on the characterization of lipid profiles of human spermatozoa by MALDI MS (16,17,30).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of n-6 and n-3 polyunsaturated acid-rich soybean phosphatidylcholine on membrane lipid profile and cryotolerance of human sperm

Vireque

Tata

Silva

et al. 2016

Fertility and Sterility

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“…Lipid attributions were based on a literature and database search. Their presence in human sperm was observed in previous reports by MALDI-MS (16,17,30).…”

Section: Lipid Profile Of Sperm Membranessupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Effects of n-6 and n-3 polyunsaturated acid-rich soybean phosphatidylcholine on membrane lipid profile and cryotolerance of human sperm

Vireque

Tata

Silva

et al. 2016

Fertility and Sterility

View full text Add to dashboard Cite

show abstract

“…Hu et al ( 27 ) reported that PC and triglycerides (TGs) possess important roles in blood lipid metabolism. As an important proinflammatory mediator, phospholipase A 2 (PLA 2 ) functions to hydrolyse PCs into lysoPCs ( 28 ). LysoPC exerts influence on numerous processes in various cell types, and may have an important role in inflammatory diseases ( 29 ).…”

Section: Introductionmentioning

confidence: 99%

Antihypertensive activity of oleanolic acid is mediated via downregulation of secretory phospholipase A2 and fatty acid synthase in spontaneously hypertensive rats

et al. 2020

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Oleanolic acid (OA) is reported to possess antihypertensive activity via the regulation of lipid metabolism; however, the mechanisms underlying lipid regulation by OA are yet to be fully elucidated. The aim of the present study was to evaluate the mechanisms via which OA regulates lipid metabolism in spontaneously hypertensive rats (SHRs) via ultra-performance liquid chromatography-quadrupole/Orbitrap-mass spectrometry (MS)-based lipidomics analysis. SHRs were treated with OA (1.08 mg/kg) for 4 weeks. The liver tissues were excised, homogenized in dichloromethane and centrifuged, and subsequently the supernatant layer was collected and concentrated under vacuum to dryness. The dichloromethane extract was subjected to MS analysis and database searching, and comparison of standards was performed to identify potential biomarkers. Partial least squares-discriminant analysis performed on the liver lipidome revealed a total of 14 endogenous metabolites that were significantly changed in the SHR model group (SH group) compared with Wistar Kyoto rats [normal control (NC group)], including glycerophospholipids, sphingolipids and glycerides. Heatmaps revealed that the liver lipid profiles in the OA group were clustered more closely compared with those observed in the NC group, indicating that the antihypertensive effect of OA was mediated via regulation of liver lipid metabolites. It was observed that the protein levels of secretory phospholipase A 2 (sPLA 2 ) and fatty acid synthase (FAS) were increased in the SH group compared with the NC group. In addition, the levels of lysophosphatidylcholine and triglycerides in the liver were elevated, whereas the levels of low-density lipoprotein cholesterol and high-density lipoprotein cholesterol were reduced in the SH group. Upon treatment with OA, the mRNA and protein levels of PLA 2 and FAS were observed to be downregulated. Collectively, the present study indicated that the antihypertensive activity of OA was mediated via downregulation of sPLA 2 and FAS in SHRs, and that treatment with OA resulted in significant improvements in blood pressure and associated abnormalities in the lipid metabolites.

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“…An accumulation of oxidation products in body tissues and cells including erythrocytes or sperm could be the result. An extensive accumulation of lysophospholipids as degradation products of phosphatidylcholines (PC) after lipid oxidation was, for example, recently observed in erythrocytes and fresh semen of severely obese men [19,20]. To assess any handicap in our samples, we looked at the occurrence of lysophosphatidylcholines (LPC) in sperm as well as in erythrocytes of the respective individuals as PCs represent the most abundant cellular membrane phospholipids.…”

Section: Introductionmentioning

confidence: 99%

Semen cryopreservation and radical reduction capacity of seminal fluid in captive African lion (Panthera leo)

et al. 2017

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Optimizing cryopreservation protocols for nondomestic felids contributes to the successful development of assisted reproduction techniques and genetic resource banking. In this study, we describe a simple cryopreservation procedure for African lion (Panthera leo) ejaculates, which was tested with different packaging options and different sperm numbers per dose. By applying urethral catheterization and electroejaculation, 17 ejaculates with greater than 20% motile and greater than 5% progressively motile sperm were collected. A lyophilized extender (a modified egg yolk-Tes-Tris-fructose-glycerol medium) was rehydrated and added in one step at ambient temperature (∼25 °C) to semen, which was prediluted in cell culture medium M199. After slow cooling of insulated samples to 15 °C in a refrigerator (4 °C), the samples were fast frozen over the surface of liquid nitrogen or in a dry shipper. Aliquots of 300 μL containing 20 × 10 sperm were frozen in cryovials and in 0.5-mL straws. Differences were observed in the total motility after thawing between vial (31.5 ± 14.1%) and straw freezing (20.1 ± 8.6%). However, the subpopulations of vital (22.7 ± 7.8% for vial and 19.8 ± 8.5% for straw) and progressively motile (10.0 ± 7.9% for vial and 10.0 ± 6.4% for straw) sperm after washing and 1 hour incubation at 38 °C were of similar magnitude, velocity, and linearity for both packaging options. After freezing of five ejaculates with 20, 60, and 100 × 10 sperm per dose, best results were achieved at the lowest concentration. In general, post-thaw results were highly variable (2.2% and 56.5% total motility) and not correlated to motility or morphology of the fresh semen. To further characterize semen quality, we assessed the protective potential of seminal fluid against oxidative stress, which might be challenged on freeze thawing. The capacity of seminal fluid to reduce radicals was measured in 10 semen samples by electron spin resonance spectroscopy and a spin-labeled fatty acid as a radical probe. Moreover, we determined the lysophosphatidylcholines (LPC) as potential lipid oxidation products in the sperm and erythrocytes of the males. Individuals with a high radical reduction capacity in the seminal fluid and a low LPC content in their erythrocytes showed a better cryosurvival of sperm. This is a first indication that seminal fluid may affect the freezing potential of African lion ejaculates.

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Enhanced lysophosphatidylcholine and sphingomyelin contents are characteristic of spermatozoa from obese men—A MALDI mass spectrometric study

Cited by 21 publications

References 38 publications

Effects of n-6 and n-3 polyunsaturated acid-rich soybean phosphatidylcholine on membrane lipid profile and cryotolerance of human sperm

Effects of n-6 and n-3 polyunsaturated acid-rich soybean phosphatidylcholine on membrane lipid profile and cryotolerance of human sperm

Antihypertensive activity of oleanolic acid is mediated via downregulation of secretory phospholipase A2 and fatty acid synthase in spontaneously hypertensive rats

Semen cryopreservation and radical reduction capacity of seminal fluid in captive African lion (Panthera leo)

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