Enhanced Neutrophil Chemotactic Activity After Bronchial Challenge in Subjects with Grain Dust-Induced Asthma

Grain dust induces IL-8 production from bronchial epithelial cells: the effect of dexamethasone on IL-8 production

“…[2][3][4] Inhalation of grain sorghum dust in normal volunteers could induce an increased peripheral neutrophil count and chemotactic response to GD. 4 In vitro study revealed the release of substances presenting neutrophil chemotactic activity by bronchial epithelial cell in response to GD.…”

“…1 Recent publications have suggested a possible role of neutrophils in the development of GDinduced airway obstruction. [2][3][4] The immunohistochemical study demonstrated that the neutrophil counts were significantly higher in the bronchial mucosa of GD-induced asthma than in that of allergic asthma. 2 The other study dealing with neutrophil chemotactic activity from subjects with GDasthma 3 revealed that serum neutrophil chemotactic activity significantly increased at 30 minutes after GD inhalation challenge and then significantly decreased at 240 minutes, whereas no significant changes occurred in asthmatic patients with negative results on GD-bronchoprovocation test.…”

Grain dust induces IL-8 production from bronchial epithelial cells: effect on neutrophil recruitment

“…[1][2][3] It was extracted with phosphate-buffered saline [(PBS, pH 7.5), 1Ϻ5 wt/vol] at 4°C for 1 hour followed by centrifugation at 5,000 rpm. The supernatants were passed through the syringe filter (MIS, USA: 0.25-m pore sized), dialyzed (cut-off molecular weight of 6,000 D) against 4 L of distilled water at 4°C for 48 hours, and lyophilized at Ϫ70°C to prepare the antigens to be used for stimulation of epithelial cells.…”

“…3 Neutrophils were obtained from heparinized whole blood of AB type normal volunteers by sedimentation in 6% dextran-dextrose solution followed by centrifugation on Ficoll-Hypaque solution (specific gravity of 1.077) and Hank's balanced salt solution (HBSS) with 0.4% of bovine serum albumin (BSA) at the concentration of 1 ϫ 10 6 cells/mL. Polycarbonate filter with 5-L pore (Millipore, Millipore, CA) was topped with chemotaxin followed by distribution of neutrophil suspension.…”

“…1 Recent publications on GD-induced asthma have suggested an active involvement of neutrophils in the development of GD-induced bronchoconstriction. [2][3][4] The immunohistochemical study demonstrated that the neutrophil counts were significantly higher in the bronchial mucosa of GD-induced asthma than in that of allergic asthma. 2 The other study dealing with neutrophil chemotactic activity from subjects with GDinduced asthma 3 revealed that serum neutrophil chemotactic activity significantly increased at 30 minutes after GD inhalation challenge and then significantly decreased at 240 minutes, whereas no significant changes occurred in asthmatic patients with negative result on GD-bronchoprovocation test.…”